Overview

  • Product nameCortisol ELISA KitSee all Cortisol kits ...
  • Detection methodColorimetric
  • Tests
    1 x 96 test
  • Sample type
    Urine
  • Assay typeCompetitive
  • Sensitivity
    2.95 ng/ml
  • Range
    10 ng/ml - 500 ng/ml
  • Recovery

    107.48 %

  • Assay durationMultiple steps standard assay
  • Species reactivity
    Reacts with: Human
  • Product overview

    Abcam’s Cortisol in vitro competitive ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate quantitative measurement of Cortisol in urine.

     

    A 96-well plate has been precoated with anti-Cortisol. Samples and the Cortisol-HRP conjugate are added to the wells, where Cortisol in the sample competes with the added Cortisol-HRP for antibody binding. After incubation, the wells are washed to remove unbound material and TMB substrate is then added which is catalyzed by HRP to produce blue coloration. The reaction is terminated by addition of Stop Solution which stops the color development and produces a color change from blue to yellow. The intensity of signal is inversely proportional to the amount of Cortisol in the sample and the intensity is measured at 450 nm.

  • Tested applicationsCompetitive ELISAmore details
  • PlatformMicroplate

Properties

    Applications

    Our Abpromise guarantee covers the use of ab108674 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    Competitive ELISA Use at an assay dependent concentration.

    Cortisol ELISA Kit images

    • Representative Standard Curve using ab108674

    Protocols

    References for Cortisol ELISA Kit (ab108674)

    ab108674 has not yet been referenced specifically in any publications.

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    This product comes with full money back guarantee if used with human urine samples. Please reassure them if the product does not work they will get refund or replacements.

    I would advise them buying 1 prod...

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    Thank you for contacting us.

    We do have several kits available against the products requested. However, none of them have unfortunately been tested with fish.

    What I would suggest is that you have a look at the products identified, ...

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    To let you know, we are currently investigating the Porcine (pig) species, cross-reactivity information for following kits ab119602, ab113326, ab119600, ab119601, ab119590 and ab119589. We will send you the de...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"