• Product nameCortisol ELISA Kit
    See all Cortisol kits
  • Detection methodColorimetric
  • Precision
    Sample n Mean SD CV%
    Urine 60 <=6.5%
    Sample n Mean SD CV%
    Urine 30 <=7.2%
  • Sample type
  • Assay typeCompetitive
  • Sensitivity
    2.95 ng/ml
  • Range
    10 ng/ml - 500 ng/ml
  • Recovery

    107.48 %

  • Assay durationMultiple steps standard assay
  • Species reactivity
    Reacts with: Human
  • Product overview

    Abcam’s Cortisol in vitro competitive ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate quantitative measurement of Cortisol in urine.

    A 96-well plate has been precoated with anti-Cortisol. Samples and the Cortisol-HRP conjugate are added to the wells, where Cortisol in the sample competes with the added Cortisol-HRP for antibody binding. After incubation, the wells are washed to remove unbound material and TMB substrate is then added which is catalyzed by HRP to produce blue coloration. The reaction is terminated by addition of Stop Solution which stops the color development and produces a color change from blue to yellow. The intensity of signal is inversely proportional to the amount of Cortisol in the sample and the intensity is measured at 450 nm.

  • Tested applicationsSuitable for: Competitive ELISAmore details
  • PlatformMicroplate


  • Storage instructionsStore at +4°C. Please refer to protocols.
  • Components 1 x 96 tests
    10X Wash Solution 1 x 50ml
    Anti-Cortisol IgG Coated Microplate (12 x 8 wells) 1 unit
    Cortisol Standard 0 - 0 ng/mL 1 x 1ml
    Cortisol Standard 1 - 10 ng/mL 1 x 1ml
    Cortisol Standard 2 - 50 ng/mL 1 x 1ml
    Cortisol Standard 3 - 150 ng/mL 1 x 1ml
    Cortisol Standard 4 – 500 ng/mL 1 x 1ml
    Cortisol-HRP Conjugate 1 x 33ml
    Cover foils 1 unit
    High control 1 x 1ml
    Low control 1 x 1ml
    Stop Solution 1 x 15ml
    Strip holder 1 unit
    TMB Substrate Solution 1 x 15ml
  • Research areas


    Our Abpromise guarantee covers the use of ab108674 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    Competitive ELISA Use at an assay dependent concentration.

    Cortisol ELISA Kit images

    • Representative Standard Curve using ab108674


    References for Cortisol ELISA Kit (ab108674)

    ab108674 has not yet been referenced specifically in any publications.

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    This product comes with full money back guarantee if used with human urine samples. Please reassure them if the product does not work they will get refund or replacements.

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