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Product name COS7 (African Green Monkey SV40-transf'd kidney fibroblast cell line) Nuclear Lysate See all COS7 lysates
General notes We recommend aliquoting the extracts into single use fractions and then
Tested applications WB, EMSA more details Properties
Storage instructions Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
Storage buffer Preservative: None
Constituents: 20% Glycerol, 20mM HEPES, 100mM Potassium Chloride, 1mM Magnesium Chloride, 0.5mM PMSF, 0.5mM DTT. pH 7.9
Concentration information loading...
Background These fibroblast-like cells grow in monolayers and are possible hosts for the propagation of pure populations of recombinant SV40 virus and contain T antigen of SV 40 virus. They are useful in virus replication studies and transfection studies.
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application notes Extracts have been quality control tested by Western blot and the Electrophoretic Mobility Shift Assay (EMSA).
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
References for COS7 (African Green Monkey SV40-transf'd kidney fibroblast cell line) Nuclear Lysate (ab14643)
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"