Overview

  • Product nameAnti-COUP TF1 antibody
    See all COUP TF1 primary antibodies
  • Description
    Rabbit polyclonal to COUP TF1
  • Tested applicationsSuitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Chicken, Cow, Pig, Zebrafish
  • Immunogen

    Synthetic peptide: GAGEQQQQAG SGAPHTPQTP GQPGAPATPG TAGDKGQGPP GSGQSQQHIE , corresponding to N terminal amino acids 36-85 of Human COUP TF1

  • Positive control
    • Transfected 293T cell lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab80698 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 50 kDa (predicted molecular weight: 46 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA Use at an assay dependent concentration.

ELISA titre using peptide based assay 1/312500.

Target

  • FunctionCoup (chicken ovalbumin upstream promoter) transcription factor binds to the ovalbumin promoter and, in conjunction with another protein (S300-II) stimulates initiation of transcription. Binds to both direct repeats and palindromes of the 5'-AGGTCA-3' motif. Represses transcriptional activity of LHCG.
  • Sequence similaritiesBelongs to the nuclear hormone receptor family. NR2 subfamily.
    Contains 1 nuclear receptor DNA-binding domain.
  • Cellular localizationNucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • Chicken ovalbumin upstream promoter 1 antibody
    • COT1_HUMAN antibody
    • COUP transcription factor 1 antibody
    • COUP transcription factor I antibody
    • COUP-TF I antibody
    • COUP-TF1 antibody
    • EAR-3 antibody
    • EAR3 antibody
    • ERBAL3 antibody
    • NR2F1 antibody
    • NR2F2 antibody
    • Nuclear receptor subfamily 2 group F member 1 antibody
    • SVP44 antibody
    • TCFCOUP1 antibody
    • TFCOUP1 antibody
    • Transcription factor COUP 1 antibody
    • V ERBA related protein EAR 3 antibody
    • V-erbA-related protein 3 antibody
    see all

Anti-COUP TF1 antibody images



  • Predicted band size : 46 kDa
    Western blot analysis of Human fetal lung tissue lysate labeling COUP TF1 with ab80698 at 1.0µg/ml.


  • Predicted band size : 46 kDa
    Western blot analysis of Human fetal brain tissue lysate labeling COUP TF1 with ab80698 at 1.0µg/ml.


  • Predicted band size : 46 kDa
    Western blot analysis of Human adult placenta tissue lysate labeling COUP TF1 with ab80698 at 1.0µg/ml.
  • Anti-COUP TF1 antibody (ab80698) at 1 µg/ml + transfected 293T cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 46 kDa
    Observed band size : 46 kDa
    Gel concentration 12%

References for Anti-COUP TF1 antibody (ab80698)

ab80698 has not yet been referenced specifically in any publications.

Product Wall

There are currently no Abreviews or Questions for ab80698.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"