Overview

  • Product nameAnti-COUP TF1 antibody
    See all COUP TF1 primary antibodies
  • Description
    Rabbit polyclonal to COUP TF1
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Sheep, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog, Pig, Drosophila melanogaster, Zebrafish
  • Immunogen

    Synthetic peptide within residues AIVLFTSDAC GLSDAAHIES LQEKSQCALE EYVRSQYPNQ PSRFGKLLLR corresponding to C terminal amino acids 323-372 of human COUP TF1 (NP_005645).

  • Positive control
    • Transfected 293T cell lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab80852 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 46 kDa (predicted molecular weight: 46 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

Target

  • FunctionCoup (chicken ovalbumin upstream promoter) transcription factor binds to the ovalbumin promoter and, in conjunction with another protein (S300-II) stimulates initiation of transcription. Binds to both direct repeats and palindromes of the 5'-AGGTCA-3' motif. Represses transcriptional activity of LHCG.
  • Sequence similaritiesBelongs to the nuclear hormone receptor family. NR2 subfamily.
    Contains 1 nuclear receptor DNA-binding domain.
  • Cellular localizationNucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • Chicken ovalbumin upstream promoter 1 antibody
    • COT1_HUMAN antibody
    • COUP transcription factor 1 antibody
    • COUP transcription factor I antibody
    • COUP-TF I antibody
    • COUP-TF1 antibody
    • EAR-3 antibody
    • EAR3 antibody
    • ERBAL3 antibody
    • NR2F1 antibody
    • NR2F2 antibody
    • Nuclear receptor subfamily 2 group F member 1 antibody
    • SVP44 antibody
    • TCFCOUP1 antibody
    • TFCOUP1 antibody
    • Transcription factor COUP 1 antibody
    • V ERBA related protein EAR 3 antibody
    • V-erbA-related protein 3 antibody
    see all

Anti-COUP TF1 antibody images

  • Anti-COUP TF1 antibody (ab80852) at 1 µg/ml (in 5% skim milk / PBS buffer) + Transfected 293T cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 46 kDa
    Observed band size : 46 kDa
    Additional bands at : 28 kDa. We are unsure as to the identity of these extra bands.

References for Anti-COUP TF1 antibody (ab80852)

ab80852 has not yet been referenced specifically in any publications.

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Zebrafish Cell (Whole mount)
Specification Whole mount
Fixative Paraformaldehyde
Permeabilization Yes - Acetone
Blocking step Serum as blocking agent for 16 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 4°C
Username

Abcam user community

Verified customer

Submitted Nov 11 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"