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Alternative versions available:
Anti-COUP TF1 antibody (Alexa Fluor® 488) [EPR10841] (ab209462)
Anti-COUP TF1 antibody (Alexa Fluor® 647) [EPR10841] (ab210038)
Anti-COUP TF1 antibody (Alexa Fluor® 568) [EPR10841] (ab211240)
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
This product is a recombinant rabbit monoclonal antibody.
Our Abpromise guarantee covers the use of ab181137 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000. Detects a band of approximately 46 kDa (predicted molecular weight: 46 kDa).|
|IHC-P||1/100 - 1/250.
The mouse and rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat.
|ICC/IF||1/100 - 1/250.|
ab172730-Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
For unpurified use at 1/70.
Immunocytochemistry/Immunofluorescence analysis of MCF-7cells labelling COUP TF1 with unpurified ab181137 at 1/500. Cells were fixed with 4% Paraformaldehyde. ab150077, an Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody.
Control: PBS only.
Nuclear counter stain: DAPI.
Immunohistochemical analysis of paraffin-embedded Human glioblastoma staining COUP TF1 using unpurified ab181137 at 1/100. Prediluted HRP Polymer for Rabbit IgG was used as a secondary antibody, and Hematoxylin for counterstaining.
Immunohistochemical analysis of paraffin-embedded Human transitional cell carcinoma staining COUP TF1 using unpurified ab181137 at 1/100. Prediluted HRP Polymer for Rabbit IgG was used as a secondary antibody, and Hematoxylin for counterstaining.
Flow cytometric analysis of COUP TF1 labeling 2% paraformaldehyde fixed 293 cells using unpurified ab181137 at 1/70 dilution (red), compared to a Rabbit monoclonal IgG isotype control (green). Goat anti rabbit IgG (FITC) secondary antibody was used at 1/150 dilution.
ab181137 has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"