Cow heart tissue lysate - mitochondrial extract (ab110338)

Overview

  • Product nameCow heart tissue lysate - mitochondrial extract
  • General notes


    2 mg of bovine heart mitochondria in iso-osmotic buffer. This sample can act as a positive control in immunocapture applications and Blue Native PAGE.

    1 mg solubilized mitochondria for Immunocapture experiments. For the Immunocapture of OXPHOS complexes, it is recommended that 180 µl of the mitochondrial membranes supplied be solubilized by the addition of 20 µl of 10% lauryl maltoside. Insoluble material is removed by centrifugation at 72,000g before immunocapture of complexes from the supernatant. For more detail see Abcam's suggested protocols.

  • Tested applicationsSuitable for: SDS-PAGE, WBmore details

Properties

  • Storage instructionsShipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
  • Storage bufferConstituents: 0.02% PMSF, 0.16% Tris, 0.89% EDTA, 8.56% Sucrose
  • Concentration information loading...
  • Lysate notes2 mg of bovine heart mitochondria in iso-osmotic buffer. This sample can act as a positive control in immunocapture applications and Blue Native PAGE.

    1 mg solubilized mitochondria for Immunocapture experiments. For the Immunocapture of OXPHOS complexes, it is recommended that 180 µl of the mitochondrial membranes supplied be solubilized by the addition of 20 µl of 10% lauryl maltoside. Insoluble material is removed by centrifugation at 72,000g before immunocapture of complexes from the supernatant. For more detail see Abcam's suggested protocols.

    Applications

    Our Abpromise guarantee covers the use of ab110338 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    SDS-PAGE Use at an assay dependent dilution.
    WB Use at an assay dependent dilution.

    References for Cow heart tissue lysate - mitochondrial extract (ab110338)

    This product has been referenced in:

    See 1 Publication for this product

    Product Wall


    Product ab110338 contains whole mitochondria in iso-osmotic buffer. There is no need to measure protein concentration of this product; the product is supplied at 5.5 mg/mL. Simply adjust the protein concentration with PBS to 5.3 mg/mL and add dete...

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    Le principe de base que nous utilisons pour la préparation d’ab110338 est le même que dans le kit http://www.abcam.com/mitochondria-isolation-kit-for-tissue-ab110168.html.

    La seule différence c’est que nous adap...

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    The ration among complexes varies from tissue to tissue. We did not know the exact number but when we validated the expression of different complexes using this product, we did observed variation across tissues.



    J’ai contacté le laboratoire concernant votre question pour ab110338. Ab110338 ne contient pas de détergent. Ce lysat devrait contenir des enzymes actives et donc pourrait être utilisé pour des tests enzymatiq...

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    Thank you for your enquiry and interest in our product.

    The concentration comes at 5.5 mg/mL (360uL). It is done in this way so that our customers can add 40uL of 10% lauryl maltoside for a final volume of 400uL at a concentration of 5 mg/mL ...

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    Thank you for your enquiry and interest in our product.

    I can confirm that the mitochondria preparation is whole mitochondria (intact) but because the mitochondria is frozen it is not coupled.

    By this I mean you are not able to perf...

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    Thank you for your reply.

    The WB looks great now - with the bright band at around 37 kDa. The band at the higher MW is very faint and seems to be specific to your sample or sample preparation as the mitochondrial control lysate does not have t...

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    Oh, I see.

    The bovine heart mito lysate was prepared with iso-osmotic buffer, thus without detergent - so that it can be used for various experiments not just WB.

    Additional information as per datasheet:
    2 mg of bovine heart mitoc...

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    Thank you for your reply.

    The lab let me know in the meantime that they think you have used too much bovine heart mito lysate. Usually, the loading amount for the bovine heart mito lysate should be at least one tenth of the cell lysates. The b...

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    Thank you for your email.

    Was the bovine heart mitochondrial lysate also prepared with 8M urea?

    I am not sure if this is what the lab recommends for denaturing. I think regular loading buffer with 1% SDS would be recommended (Laemmli ...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"