Overview

  • Product nameAnti-Cox 1+2 antibody
  • Description
    Goat polyclonal to Cox 1+2
  • Specificityab35995 recognises COX 1 and 2 (PGHS1/2). It reacts moderately with COX2 from most mammalian species, negligible reactivity is seen with chicken COX2 at given dilution. Reacts weakly with Chicken. Antibody reactivity and working conditions may vary between species.
  • Tested applicationsWB, ELISAmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Sheep
    Predicted to work with: all Mammals
  • Immunogen

    Full length protein corresponding to Sheep Cox 1+2.
    Database link: P05979

  • General notes

    Storage in frost-free freezers is not recommended.
    This product should be stored undiluted.

    Should this product contain a precipitate we recommend microcentrifugation before use.

Properties

Applications

Our Abpromise guarantee covers the use of ab35995 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/2000. Predicted molecular weight: 69 kDa.
ELISA Use at an assay dependent dilution.

Target

  • RelevanceCyclooxygenase (COX), also known as Prostaglandin H2 synthase and Prostaglandin endoperoxide synthase, is a key enzyme in the of conversion arachidonic acid to Prostaglandin H2. Prostaglandin H2 is converted by other enzymes into inflammatory mediators prostaglandin (PG) D2, PGE2, PGF2a, PGI2 and Thromboxane A2. Thus, COX is a key enzyme in the production of inflammatory agents and is the target of intense research and drug discovery activities. There are two enzymes of COX, COX 1 and COX 2. COX 1 is constitutively produced in many (most) cell types. It is important in the gut for the production of prostaglandins, which inhibit gastric secretion. It can be induced in monocytes, macrophages and other cells. It is induced as part of the inflammatory response by IL 1beta and other cytokines. It is induced by growth factors such as EGF and PDGF. Expression is inhibited by glucocorticosteroids such as cortisol and dexamethasone. Lipopolysaccharide in bacterial infections induces COX 2. COX 2 is also found in elevated levels in synoviocytes from rheumatoid arthritis patients. The discomforts of inflammation such as pain and swelling are largely due to the action of prostaglandins produced by COX-2. COX2 (Cyclooxygenase 2) is an inducible enzyme. It is involved in the response of cells to growth factors, tumor promoters, and cytokines that induce its expression. Given its role in synthesizing prostaglandins, COX2 is therefore of interest in studying immune response regulation. COX2 is induced by a wide variety of stimuli and was initially identified as immediate early growth response gene. In addition, COX2 expression markedly increased in 85-90% of human colorectal adenocarcinoma whereas COX1 levels remain unchanged.
  • Cellular localizationMicrosomal membrane
  • Database links
  • Alternative names
    • COX 2 antibody
    • COX3 antibody
    • Cyclooxygenase 1 antibody
    • PCOX 1 antibody
    • PGG/HS antibody
    • PGH synthase 2 antibody
    • PGHS 1 antibody
    • PHS II antibody
    • Prostaglandin endoperoxide synthase 1 antibody
    • Prostaglandin endoperoxide synthase 2 antibody
    • PTGS2 antibody
    • TIS10 antibody
    see all

References for Anti-Cox 1+2 antibody (ab35995)

This product has been referenced in:

See 1 Publication for this product

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