The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 - 4 µg/ml. Predicted molecular weight: 30 kDa.
RelevanceCOX proteins are membrane-associated heme proteins that have cyclooxygenase and peroxidase activities. These enzymes are targets of NSAID (nonsteroidal anti-inflammatory drugs) such as aspirin. Prostaglandins (PGs) formed by the enzymatic activity of COX-1 are primarily involved in the regulation of homeostatic functions throughout the body, whereas PGs formed by COX-2 primarily mediate pain, fever, and inflammation. COX-1 is constitutively expressed, with particularly high expression in gastrointestinal tissues. COX-2 is induced by cytokines and mitogens and is likely to play a role in inflammatory diseases such as rheumatoid arthritis. In rodents and humans, COX-3 encodes proteins with completely different amino acid sequences from COX-1 or COX-2, and without COX activity. COX-3 is thought to be a splice variant of COX-1 which retains intron one. It has been suggested that COX-3 may be the key to unlocking the mechanism of action of acetaminophen.
Cui TZ et al. Modulation of the respiratory supercomplexes in yeast: enhanced formation of cytochrome oxidase increases the stability and abundance of respiratory supercomplexes. J Biol Chem289:6133-41 (2014).
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