Anti-COX IV [4D11-B3-E8] antibody (ab131177)

Overview

  • Product nameAnti-COX IV [4D11-B3-E8] antibodySee all COX IV primary antibodies ...
  • Description
    Mouse monoclonal [4D11-B3-E8] to COX IV
  • Specificityab131177 detects endogenous levels of COXIV Isoform 1 and does not cross-react with related proteins.
  • Tested applicationsIP, IHC-P, ICC/IF, WB, ELISA, Flow Cyt more details
  • Species reactivity
    Reacts with: Mouse, Rat, Goat, Human, African Green Monkey, Chinese Hamster
  • Immunogen

    Synthetic peptide of Human COXIV Isoform 1.

  • Positive control
    • HeLa cells; Mouse skeletal muscle and Jurkat cell lysates; K562 cells for flow cytometry.

Properties

Applications

Our Abpromise guarantee covers the use of ab131177 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
IP Use at an assay dependent concentration.
IHC-P Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF Use at an assay dependent concentration.
WB 1/1000. Detects a band of approximately 20 kDa (predicted molecular weight: 20 kDa).
ELISA Use at an assay dependent concentration.
Flow Cyt 1/100.

Target

  • FunctionThis protein is one of the nuclear-coded polypeptide chains of cytochrome c oxidase, the terminal oxidase in mitochondrial electron transport.
  • Tissue specificityUbiquitous.
  • Sequence similaritiesBelongs to the cytochrome c oxidase IV family.
  • Cellular localizationMitochondrion inner membrane.
  • Target information above from: UniProt accession P13073 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links
  • Alternative names
    • AL024441 antibody
    • COX 4 antibody
    • COX IV 1 antibody
    • COX IV antibody
    • COX IV-1 antibody
    • Cox4 antibody
    • COX41_HUMAN antibody
    • Cox4a antibody
    • COX4B antibody
    • COX4I1 antibody
    • COX4I2 antibody
    • COX4L2 antibody
    • COXIV antibody
    • Cytochrome c oxidase polypeptide IV antibody
    • Cytochrome c oxidase subunit 4 isoform 1 mitochondrial antibody
    • Cytochrome c oxidase subunit 4 isoform 1, mitochondrial antibody
    • Cytochrome C Oxidase subunit IV antibody
    • Cytochrome c oxidase subunit IV isoform 1 antibody
    • Cytochrome c oxidase subunit IV isoform 2 (lung) antibody
    • Cytochrome c oxydase subunit 4 antibody
    • dJ857M17.2 antibody
    • MGC105470 antibody
    • MGC72016 antibody
    see all

Anti-COX IV [4D11-B3-E8] antibody images

  • Immunohistochemical analysis of paraffin-embedded Human colorectal carcinoma with COX IV Mouse mAb [4D11-B3-E8] (1:50),showing cytoplasm localization. A high pressure mediated antigen retrieval step was performed in citrate buffer(pH6.0).

  • All lanes : Anti-COX IV [4D11-B3-E8] antibody (ab131177) at 1/5000 dilution

    Lane 1 : COS7 cell lysate
    Lane 2 : 3T3 cell lysate
    Lane 3 : K562 cell lysate
    Lane 4 : C6 cell lysate
    Lane 5 : Goat muscle cell lysate
    Lane 6 : CHO-K1 cell lysate
    Lane 7 : HeLa cell lysate


    Predicted band size : 20 kDa
    Observed band size : 17 kDa (why is the actual band size different from the predicted?)
  • Immunofluorescent analysis of HeLa cells labelling COXIV Isoform 2 with ab131177 at 1/150 dilution.
  • Immunoprecipitation analysis of HeLa cell lysate labeling COX IV with ab131177.

  • All lanes : Anti-COX IV [4D11-B3-E8] antibody (ab131177) at 1/1000 dilution

    Lane 1 : Mouse skeletal muscle lysate
    Lane 2 : Jurkat cell lysate


    Predicted band size : 20 kDa
  • Flow Cytometric analysis of K562 cells labelling COXIV Isoform 2 with ab131177 (red) at 1/100 dilution; followed by FITC-conjugated goat anti-mouse IgG. Mouse IgG control (blue).

References for Anti-COX IV [4D11-B3-E8] antibody (ab131177)

ab131177 has not yet been referenced specifically in any publications.

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