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Synthetic peptide of Human COXIV Isoform 1.
Our Abpromise guarantee covers the use of ab131177 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IP||Use at an assay dependent concentration.|
|IHC-P||Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|ICC/IF||Use at an assay dependent concentration.|
|WB||1/1000. Detects a band of approximately 20 kDa (predicted molecular weight: 20 kDa).|
|ELISA||Use at an assay dependent concentration.|
|Flow Cyt||1/100. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.|
Immunohistochemical analysis of paraffin-embedded Human colorectal carcinoma with COX IV Mouse mAb [4D11-B3-E8] (1:50),showing cytoplasm localization. A high pressure mediated antigen retrieval step was performed in citrate buffer(pH6.0).
Immunoprecipitation analysis of HeLa cell lysate labeling COX IV with ab131177.
ab131177 has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"