Anti-COX IV antibody (ab63947)

Overview

  • Product nameAnti-COX IV antibody
    See all COX IV primary antibodies
  • Description
    Goat polyclonal to COX IV
  • Tested applicationsSuitable for: IHC-P, WB, ELISAmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Chimpanzee, Baboon, Gorilla, Orangutan
  • Immunogen

    Synthetic peptide corresponding to Human COX IV aa 154-166 (C terminal).
    Sequence:

    QGLASKWDYEKNE

  • Positive control
    • Human Muscle lysates, human thyroid gland tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab63947 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 2 - 4 mg/ml.
WB Use a concentration of 0.01 - 0.03 µg/ml. Detects a band of approximately 18 kDa.
ELISA 1/4000.

Target

  • FunctionThis protein is one of the nuclear-coded polypeptide chains of cytochrome c oxidase, the terminal oxidase in mitochondrial electron transport.
  • Tissue specificityUbiquitous.
  • Sequence similaritiesBelongs to the cytochrome c oxidase IV family.
  • Cellular localizationMitochondrion inner membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • AL024441 antibody
    • COX 4 antibody
    • COX IV 1 antibody
    • COX IV antibody
    • COX IV-1 antibody
    • Cox4 antibody
    • COX41_HUMAN antibody
    • Cox4a antibody
    • COX4B antibody
    • COX4I1 antibody
    • COX4I2 antibody
    • COX4L2 antibody
    • COXIV antibody
    • Cytochrome c oxidase polypeptide IV antibody
    • Cytochrome c oxidase subunit 4 isoform 1 mitochondrial antibody
    • Cytochrome c oxidase subunit 4 isoform 1, mitochondrial antibody
    • Cytochrome C Oxidase subunit IV antibody
    • Cytochrome c oxidase subunit IV isoform 1 antibody
    • Cytochrome c oxidase subunit IV isoform 2 (lung) antibody
    • Cytochrome c oxydase subunit 4 antibody
    • dJ857M17.2 antibody
    • MGC105470 antibody
    • MGC72016 antibody
    see all

Anti-COX IV antibody images

  • Anti-COX IV antibody (ab63947) at 0.01 µg/ml (1 hour) + Human Muscle lysate in RIPA buffer at 35 µg
    Developed using the ECL technique

    Observed band size : 18 kDa (why is the actual band size different from the predicted?)
  • Immunohistochemical analysis of formalin/PFA-fixed paraffin-embedded human thyroid gland tissue sections labeling COXIV with ab63947 at 2.5 µg/mL. Tissue underwent antigen retrieval in citrate buffer pH 6.0. A AP-staining procedure was used for tissue staininng.

References for Anti-COX IV antibody (ab63947)

ab63947 has not yet been referenced specifically in any publications.

Product Wall

Application Western blot
Sample Human Cell lysate - other (Mitochondrial fractions of HeLa WT and HeLa rho-0)
Loading amount 26 µg
Specification Mitochondrial fractions of HeLa WT and HeLa rho-0
Gel Running Conditions Reduced Denaturing (4-15%)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
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Submitted Oct 26 2012

Thank you for contacting us.
Unfortunately, ab110261 has been tested and found to be unsuitable for use on mouse samples, and ab63947 was raised against a very short immunogen that shows only 69% homology with mouse. We would not expect either of ...

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