Anti-COX IV antibody (ab63947)

Overview

  • Product name
  • Description
    Goat polyclonal to COX IV
  • Tested applications
    Suitable for: IHC-P, WB, ELISAmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Chimpanzee, Baboon, Gorilla, Orangutan
  • Immunogen

    Synthetic peptide corresponding to Human COX IV aa 154-166 (C terminal).
    Sequence:

    QGLASKWDYEKNE

  • Positive control
    • Human Muscle lysates, human thyroid gland tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab63947 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 2 - 4 mg/ml.
WB Use a concentration of 0.01 - 0.03 µg/ml. Detects a band of approximately 18 kDa.
ELISA 1/4000.

Target

  • Function
    This protein is one of the nuclear-coded polypeptide chains of cytochrome c oxidase, the terminal oxidase in mitochondrial electron transport.
  • Tissue specificity
    Ubiquitous.
  • Sequence similarities
    Belongs to the cytochrome c oxidase IV family.
  • Cellular localization
    Mitochondrion inner membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • AL024441 antibody
    • COX 4 antibody
    • COX IV 1 antibody
    • COX IV antibody
    • COX IV-1 antibody
    • Cox4 antibody
    • COX41_HUMAN antibody
    • Cox4a antibody
    • COX4B antibody
    • COX4I1 antibody
    • COX4I2 antibody
    • COX4L2 antibody
    • COXIV antibody
    • Cytochrome c oxidase polypeptide IV antibody
    • Cytochrome c oxidase subunit 4 isoform 1 mitochondrial antibody
    • Cytochrome c oxidase subunit 4 isoform 1, mitochondrial antibody
    • Cytochrome C Oxidase subunit IV antibody
    • Cytochrome c oxidase subunit IV isoform 1 antibody
    • Cytochrome c oxidase subunit IV isoform 2 (lung) antibody
    • Cytochrome c oxydase subunit 4 antibody
    • dJ857M17.2 antibody
    • MGC105470 antibody
    • MGC72016 antibody
    see all

Images

  • Anti-COX IV antibody (ab63947) at 0.01 µg/ml (1 hour) + Human Muscle lysate in RIPA buffer at 35 µg
    Developed using the ECL technique

    Observed band size : 18 kDa (why is the actual band size different from the predicted?)
  • Immunohistochemical analysis of formalin/PFA-fixed paraffin-embedded human thyroid gland tissue sections labeling COXIV with ab63947 at 2.5 µg/mL. Tissue underwent antigen retrieval in citrate buffer pH 6.0. A AP-staining procedure was used for tissue staininng.

References

ab63947 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Application
Western blot
Sample
Human Cell lysate - other (Mitochondrial fractions of HeLa WT and HeLa rho-0)
Loading amount
26 µg
Specification
Mitochondrial fractions of HeLa WT and HeLa rho-0
Gel Running Conditions
Reduced Denaturing (4-15%)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
Username

Abcam user community

Verified customer

Submitted Oct 26 2012

Thank you for contacting us.
Unfortunately, ab110261 has been tested and found to be unsuitable for use on mouse samples, and ab63947 was raised against a very short immunogen that shows only 69% homology with mouse. We would not expect either of ...

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