Western blot abreview for Anti-COX IV [mAbcam33985] antibody

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Xenopus laevis Cell lysate - whole cell (egg extract)
Loading amount 15 µg
Specification egg extract
Gel Running Conditions Reduced Denaturing
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C

Other product details

Dilution 1/1000
Incubation time 14 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: TBST / 5% BSA

Secondary antibody

Name Non-Abcam antibody was used: Donkey anti-mouse IgG
Host species: Donkey
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution 1/4000


Detection method ECL
Exposure 1 hour(s), 30 minute(s) and 0 second(s)
Bands Specific: 15 (weak) kDa

Dr. Anne-Lore Schlaitz

Verified customer

Submitted Aug 21 2008