Western blot abreview for Anti-COX IV [mAbcam33985] antibody

Good
Abreviews
Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Xenopus laevis Cell lysate - whole cell (egg extract)
Loading amount
15 µg
Specification
egg extract
Gel Running Conditions
Reduced Denaturing
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C

Other product details

Dilution
1/1000
Incubation time
14 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: TBST / 5% BSA

Secondary antibody

Name
Non-Abcam antibody was used: Donkey anti-mouse IgG
Host species: Donkey
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/4000

Detection

Detection method
ECL
Exposure
1 hour(s), 30 minute(s) and 0 second(s)
Bands
Specific: 15 (weak) kDa
Username

Dr. Anne-Lore Schlaitz

Verified customer

Submitted Aug 21 2008

Sign up