COX1 / Cyclooxygenase 1 293T Transfected Lysate - (positive control) (ab94213)

Overview

  • Product nameCOX1 / Cyclooxygenase 1 293T Transfected Lysate - (positive control)
  • General notesab94213 is a 293T cell transfected lysate in which Human COX1 / Cyclooxygenase 1 has been transiently over-expressed using a pCMV-COX1 / Cyclooxygenase 1 plasmid. The lysate is provided in 1X Sample Buffer. Note: For more details about how the transfected lysate was prepared view preparation notes
  • Tested applicationsWBmore details

Properties

  • BackgroundFunction: May play an important role in regulating or promoting cell proliferation in some normal and neoplastically transformed cells. Pathway: Lipid metabolism; prostaglandin biosynthesis. Similarity: Belongs to the prostaglandin G/H synthase family. Contains 1 EGF-like domain.

Applications

Our Abpromise guarantee covers the use of ab94213 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
  • Application notesWB: Use at an assay dependent dilution.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • COX1 / Cyclooxygenase 1 293T Transfected Lysate - (positive control) images

    • ab94213 at 15µg/lane on an SDS-PAGE gel
    • All lanes : Anti-COX1 / Cyclooxygenase 1 antibody (ab89630) at 1/500 dilution

      Lane 1 : COX1 / Cyclooxygenase 1 293T Transfected Lysate - (positive control) (ab94213)
      Lane 2 : 293T non-transfected lysate

      Lysates/proteins at 25 µg per lane.

      Secondary
      Goat Anti-mouse IgG (H and L) HRP conjugated at 1/2500 dilution

    References for COX1 / Cyclooxygenase 1 293T Transfected Lysate - (positive control) (ab94213)

    ab94213 has not yet been referenced specifically in any publications.

    Product Wall

    Thank you for your enquiry and for sending along the attached western blot images.

    Here are some additionalcomments and questions regarding the images:

    Slide 1:
    1. In which lane did you run the positive control lysate, ab94213?...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"