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A preparation of intracellular proteins of the human cell line HL60.
Our Abpromise guarantee covers the use of ab695 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ELISA||Use a concentration of 0.001 - 0.01 µg/ml.|
|WB||Use a concentration of 1 - 2 µg/ml.|
|IHC-Fr||Use a concentration of 10 µg/ml.|
|IHC-P||Use at an assay dependent dilution.|
|Flow Cyt||Use 1µg for 106 cells.|
|IP||Use at an assay dependent dilution.|
|ICC/IF||Use at an assay dependent concentration.|
Lane 1: MM standard
Lane 2: Cox-1 (protein standard prepared from ram seminal vesicles, supplied by Alpha Diagnostic International)
Antibody 5F6/F4: 1
ab695 at 1/400 staining human anal cancer tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed before the tissue was incubated with the antibody for 45 minutes. An HRP conjugated goat anti-mouse antibody was used as the secondary.
This image is courtesy of an anonymous abreview.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"