Anti-COX1 / Cyclooxygenase 1 antibody [5F6/F4] (ab695)

Overview

  • Product nameAnti-COX1 / Cyclooxygenase 1 antibody [5F6/F4]See all COX1 / Cyclooxygenase 1 primary antibodies ...
  • Description
    Mouse monoclonal [5F6/F4] to COX1 / Cyclooxygenase 1
  • SpecificityCOX-1. No cross-reactivity with Cox-2.
  • Tested applicationsELISA, WB, IHC-Fr, IHC-P, Flow Cyt, IP, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    A preparation of intracellular proteins of the human cell line HL60.

  • General notesPlease note that we have received reports from customers working with this antibody that they obtain two stong bands in Western blot. A single band at around 72kDa is expected. One customer reported that when tested with three different human cell lines two strong bands are obtained, one between 40 - 50kDa and other other between 62 - 83kDa. It is possible that a degraded form of the enzyme is detected or the bands may correspond to different glycosylation states.

Properties

Applications

Our Abpromise guarantee covers the use of ab695 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use a concentration of 0.001 - 0.01 µg/ml.
WB Use a concentration of 1 - 2 µg/ml.
IHC-Fr Use a concentration of 10 µg/ml.
IHC-P Use at an assay dependent dilution.
Flow Cyt Use 1µg for 106 cells.
IP Use at an assay dependent dilution.
ICC/IF Use at an assay dependent concentration.

Target

  • FunctionMay play an important role in regulating or promoting cell proliferation in some normal and neoplastically transformed cells.
  • PathwayLipid metabolism; prostaglandin biosynthesis.
  • Sequence similaritiesBelongs to the prostaglandin G/H synthase family.
    Contains 1 EGF-like domain.
  • Cellular localizationMicrosome membrane. Endoplasmic reticulum membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • COX 1 antibody
    • COX 3 antibody
    • COX-1 antibody
    • COX1 antibody
    • COX3 antibody
    • Cyclooxygenase 1 antibody
    • Cyclooxygenase 3, included antibody
    • Cyclooxygenase-1 antibody
    • Cyclooxygenase1 antibody
    • EC 1.14.99.1 antibody
    • Partial COX1 proteins, included antibody
    • PCOX 1 antibody
    • PCOX1 antibody
    • PGG/HS antibody
    • PGH synthase 1 antibody
    • PGH1_HUMAN antibody
    • PGHS 1 antibody
    • PGHS-1 antibody
    • PGHS1 antibody
    • PHS 1 antibody
    • PHS1 antibody
    • Prostaglandin endoperoxide synthase 1 antibody
    • Prostaglandin G/H synthase 1 antibody
    • Prostaglandin G/H synthase and cyclooxygenase antibody
    • Prostaglandin H Synthase 1 antibody
    • Prostaglandin H2 synthase 1 antibody
    • Prostaglandin-endoperoxide synthase 1 (prostaglandin G/H synthase and cyclooxygenase) antibody
    • Prostaglandin-endoperoxide synthase 1 antibody
    • PTGHS antibody
    • PTGS 1 antibody
    • PTGS1 antibody
    see all

Anti-COX1 / Cyclooxygenase 1 antibody [5F6/F4] images

  • Lane 1: MM standard
    Lane 2: Cox-1 (protein standard prepared from ram seminal vesicles, supplied by Alpha Diagnostic International)
    Antibody 5F6/F4: 1 µg/ml

  • ab695 at 1/400 staining human anal cancer tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed before the tissue was incubated with the antibody for 45 minutes. An HRP conjugated goat anti-mouse antibody was used as the secondary.

    See Abreview

  • Anti-COX1 / Cyclooxygenase 1 antibody [5F6/F4] (ab695) at 1 µg/ml + whole cell lysate prepared from human huh-7 cell line at 15 µg

    Secondary
    Sheep anti-mouse IgG conjugated to HRP at 1/1000 dilution

    Performed under reducing conditions.

    Observed band size : 68 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 38 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 4 minutes

    This image is courtesy of an anonymous abreview.

    See Abreview

  • Overlay histogram showing NIH3T3 cells stained with ab695 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab695, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

  • ab695 staining COX1 / Cyclooxygenase 1 in the Mouse embryonic fibroblasts (MEF)cell line by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with Triton X-100 1% in PBS and blocked with 5% serum for 60 minutes at 21°C. Samples were incubated with primary antibody (1/100 in 5% Goat serum) for 2 hours. An Alexa Fluor®546-conjugated Goat anti-mouse IgG polyclonal(1/400) was used as the secondary antibody.

    See Abreview

  • ab695 staining COX1 / Cyclooxygenase 1 in Mouse heart tissue sections by Immunohistochemistry (IHC-Fr - frozen sections). Tissue was fixed with paraformaldehyde and blocked with 5% serum for 60 minutes at 25°C. Samples were incubated with primary antibody (1/200 in 5% Goat serum) for 2 hours. An Alexa Fluor®488-conjugated Goat anti-rabbit IgG polyclonal (1/400) was used as the secondary antibody.

    See Abreview

References for Anti-COX1 / Cyclooxygenase 1 antibody [5F6/F4] (ab695)

This product has been referenced in:
  • Carlson LM  et al. Low-dose aspirin delays an inflammatory tumor progression in vivo in a transgenic mouse model of neuroblastoma. Carcinogenesis 34:1081-8 (2013). IHC ; Mouse . Read more (PubMed: 23349014) »
  • Eilati E  et al. Flaxseed enriched diet-mediated reduction in ovarian cancer severity is correlated to the reduction of prostaglandin E2 in laying hen ovaries. Prostaglandins Leukot Essent Fatty Acids 89:179-87 (2013). WB . Read more (PubMed: 23978451) »

See all 4 Publications for this product

Product Wall

Application Western blot
Loading amount 30 µg
Gel Running Conditions Reduced Denaturing
Sample Human Cell lysate - whole cell (hek293)
Specification hek293
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: rt°C
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Submitted Oct 14 2013

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Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: CITRATE BUFFER
Sample Mouse Tissue sections (MOUSE HEART)
Specification MOUSE HEART
Permeabilization Yes - 1% TRITON X-100
Fixative Paraformaldehyde
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Submitted Jul 02 2013

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Application Western blot
Sample Rat Cell lysate - whole cell (H9C2 cell)
Loading amount 30 µg
Specification H9C2 cell
Gel Running Conditions Reduced Denaturing
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: rt°C
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Submitted Mar 12 2013

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Application Immunohistochemistry (Frozen sections)
Sample Mouse Tissue sections (mouse heart)
Specification mouse heart
Fixative Paraformaldehyde
Permeabilization No
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: rt°C
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Submitted Mar 06 2013

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Application Immunocytochemistry/ Immunofluorescence
Sample Mouse Cell (mef)
Specification mef
Fixative Paraformaldehyde
Permeabilization Yes - 1% triton x100
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: rt°C
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Submitted Mar 01 2013

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Application Western blot
Sample Mouse Tissue lysate - whole (Heart)
Loading amount 4 µg
Specification Heart
Gel Running Conditions Non-reduced Denaturing (10%)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
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Submitted Jul 08 2011

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Application Immunoprecipitation
Sample Human Cell lysate - whole cell (huh-7)
Total protein in input 1e+006 cells
Specification huh-7
Treatment cells expressing recombitant myc-tagged COX1
Immuno-precipitation step Protein A
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Submitted Jun 18 2010

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Application Western blot
Sample Human Cell lysate - whole cell (huh-7 cell line)
Loading amount 15 µg
Specification huh-7 cell line
Gel Running Conditions Reduced Denaturing
Blocking step Milk as blocking agent for 16 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
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Submitted Nov 27 2009

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Application Immunoprecipitation
Sample Human Cell lysate - whole cell (293T)
Total protein in input 200 µg
Specification 293T
Immuno-precipitation step Protein G
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Submitted Dec 07 2007

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Application Western blot
Sample Human Cell lysate - whole cell (U2OS and 293T cell line)
Loading amount 30 µg
Specification U2OS and 293T cell line
Gel Running Conditions Reduced Denaturing
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
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Submitted Nov 07 2007

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"