Anti-COX1 / Cyclooxygenase 1 antibody [EPR5866] (ab109025)
- Product nameAnti-COX1 / Cyclooxygenase 1 antibody [EPR5866]See all COX1 / Cyclooxygenase 1 primary antibodies ...
- DescriptionRabbit monoclonal [EPR5866] to COX1 / Cyclooxygenase 1
- Tested applicationsWB, IP, IHC-P, ICC/IF, Flow Cyt more details
- Species reactivityReacts with: Mouse, Rat, Human
Synthetic peptide corresponding to residues in Human COX1 / Cyclooxygenase 1.
- Positive control
- NIH3T3, HACAT, Neuro -2a, C2C12, A431, and L6 cell lysates; Human skin tissue; HeLa cells.
- General notesProduced under U.S. Patent No. 5,675,063.
- Storage instructionsStore at -20°C. Stable for 12 months at -20°C
- Dissociation constant (KD) KD = 5.50 x 10 -12 M Learn more about KD
- Storage bufferPBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
- PurityTissue culture supernatant
- Clonality Monoclonal
- Clone numberEPR5866
- Pathways and Processes
- Metabolic signaling pathways
- Lipid and lipoprotein metabolism
- Lipoprotein metabolism
Our Abpromise guarantee covers the use of ab109025 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||WB: 1/1000 - 1/10000. Predicted molecular weight: 69 kDa.|
|IP||IP: 1/10 - 1/100.|
|IHC-P||IHC-P: 1/250 - 1/500. Heat up to 98 °C, below boiling, and then let cool for 10-20 min.|
|ICC/IF||ICC/IF: 1/100 - 1/250.|
|Flow Cyt||Flow Cyt: 1/10 - 1/100.|
- FunctionMay play an important role in regulating or promoting cell proliferation in some normal and neoplastically transformed cells.
- PathwayLipid metabolism; prostaglandin biosynthesis.
- Sequence similaritiesBelongs to the prostaglandin G/H synthase family.
Contains 1 EGF-like domain.
- Cellular localizationMicrosome membrane. Endoplasmic reticulum membrane.
- COX 1 antibody
- COX 3 antibody
- COX-1 antibody
- COX1 antibody
- Cox3 antibody
- Cyclooxygenase 1 antibody
- Cyclooxygenase 3, included antibody
- Cyclooxygenase-1 antibody
- EC 184.108.40.206 antibody
- Partial COX1 proteins, included antibody
- PCOX1 antibody
- PGG/HS antibody
- PGH synthase 1 antibody
- PGH1_HUMAN antibody
- PGHS-1 antibody
- PGHS1 antibody
- PHS 1 antibody
- PHS1 antibody
- Prostaglandin G/H synthase 1 antibody
- Prostaglandin H2 synthase 1 antibody
- Prostaglandin-endoperoxide synthase 1 (prostaglandin G/H synthase and cyclooxygenase) antibody
- Prostaglandin-endoperoxide synthase 1 antibody
- PTGHS antibody
- PTGS1 antibody
Anti-COX1 / Cyclooxygenase 1 antibody [EPR5866] images
All lanes : Anti-COX1 / Cyclooxygenase 1 antibody [EPR5866] (ab109025) at 1/1000 dilution
Lane 1 : NIH3T3 cell lysate
Lane 2 : HACAT cell lysate
Lane 3 : Neuro 2a cell lysate
Lane 4 : C2C12 cell lysate
Lane 5 : A431 cell lysate
Lane 6 : L6 cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size : 69 kDa
ab109025 at 1/250 dilution staining COX1 / Cyclooxygenase 1 in Human skin by Immunohistochemistry, Paraffin-embedded tissue.
ab109025 at 1/100 dilution staining COX1 / Cyclooxygenase 1 in HeLa cells by Immunofluorescence.
Overlay histogram showing NIH3T3 cells stained with ab109025 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab109025, 1/100 dilution) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (ab96899) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.
Equilibrium disassociation constant (KD)
Learn more about KD
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References for Anti-COX1 / Cyclooxygenase 1 antibody [EPR5866] (ab109025)
This product has been referenced in:
- Caligiuri SP et al. Dietary linoleic acid and a-linolenic acid differentially affect renal oxylipins and phospholipid fatty acids in diet-induced obese rats. J Nutr 143:1421-31 (2013). Read more (PubMed: 23902961) »
- McLarty JL et al. Estrogenic modulation of inflammation-related genes in male rats following volume overload. Physiol Genomics 44:362-73 (2012). WB ; Rat . Read more (PubMed: 22274565) »