RabMAb

Anti-COX1 / Cyclooxygenase 1 antibody [EPR5866] (ab109025)

Overview

  • Product nameAnti-COX1 / Cyclooxygenase 1 antibody [EPR5866]See all COX1 / Cyclooxygenase 1 primary antibodies ...
  • Description
    Rabbit monoclonal [EPR5866] to COX1 / Cyclooxygenase 1
  • Tested applicationsWB, IP, IHC-P, ICC/IF, Flow Cytmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide corresponding to residues in Human COX1/ Cyclooxygenase 1.

  • Positive control
    • NIH3T3, HACAT, Neuro -2a, C2C12, A431, and L6 cell lysates; Human skin tissue; HeLa cells.
  • General notesProduced under U.S. Patent No. 5,675,063.

Applications

Our Abpromise guarantee covers the use of ab109025 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 69 kDa.
IP 1/10 - 1/100.
IHC-P 1/250 - 1/500. Heat up to 98 °C, below boiling, and then let cool for 10-20 min.
ICC/IF 1/100 - 1/250.
Flow Cyt 1/10 - 1/100.

Target

  • FunctionMay play an important role in regulating or promoting cell proliferation in some normal and neoplastically transformed cells.
  • PathwayLipid metabolism; prostaglandin biosynthesis.
  • Sequence similaritiesBelongs to the prostaglandin G/H synthase family.
    Contains 1 EGF-like domain.
  • Cellular localizationMicrosome membrane. Endoplasmic reticulum membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • COX 1 antibody
    • COX 3 antibody
    • COX-1 antibody
    • COX1 antibody
    • Cox3 antibody
    • Cyclooxygenase 1 antibody
    • Cyclooxygenase 3, included antibody
    • Cyclooxygenase-1 antibody
    • EC 1.14.99.1 antibody
    • Partial COX1 proteins, included antibody
    • PCOX1 antibody
    • PGG/HS antibody
    • PGH synthase 1 antibody
    • PGH1_HUMAN antibody
    • PGHS-1 antibody
    • PGHS1 antibody
    • PHS 1 antibody
    • PHS1 antibody
    • Prostaglandin G/H synthase 1 antibody
    • Prostaglandin H2 synthase 1 antibody
    • Prostaglandin-endoperoxide synthase 1 (prostaglandin G/H synthase and cyclooxygenase) antibody
    • Prostaglandin-endoperoxide synthase 1 antibody
    • PTGHS antibody
    • PTGS1 antibody
    see all

Anti-COX1 / Cyclooxygenase 1 antibody [EPR5866] images

  • All lanes : Anti-COX1 / Cyclooxygenase 1 antibody [EPR5866] (ab109025) at 1/1000 dilution

    Lane 1 : NIH3T3 cell lysate
    Lane 2 : HACAT cell lysate
    Lane 3 : Neuro 2a cell lysate
    Lane 4 : C2C12 cell lysate
    Lane 5 : A431 cell lysate
    Lane 6 : L6 cell lysate

    Lysates/proteins at 10 µg per lane.


    Predicted band size : 69 kDa
  • ab109025 at 1/250 dilution staining COX1 / Cyclooxygenase 1 in Human skin by Immunohistochemistry, Paraffin-embedded tissue.
  • ab109025 at 1/100 dilution staining COX1 / Cyclooxygenase 1 in HeLa cells by Immunofluorescence.
  • Overlay histogram showing NIH3T3 cells stained with ab109025 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab109025, 1/100 dilution) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (ab96899) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

  • Equilibrium disassociation constant (KD)
    Learn more about KD

    Click here to learn more about KD

References for Anti-COX1 / Cyclooxygenase 1 antibody [EPR5866] (ab109025)

This product has been referenced in:
  • Caligiuri SP  et al. Dietary linoleic acid and a-linolenic acid differentially affect renal oxylipins and phospholipid fatty acids in diet-induced obese rats. J Nutr 143:1421-31 (2013). Read more (PubMed: 23902961) »
  • McLarty JL  et al. Estrogenic modulation of inflammation-related genes in male rats following volume overload. Physiol Genomics 44:362-73 (2012). WB ; Rat . Read more (PubMed: 22274565) »

See all 2 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Mouse Tissue lysate - whole (mouse heart)
Loading amount 30 µg
Specification mouse heart
Gel Running Conditions Reduced Denaturing
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: rt°C
Username

Abcam user community

Verified customer

Submitted Apr 08 2013

Thank you for your inquiry.

I am happy toconfirm that the image on the datasheet was produced using Citrate buffer pH6 for antigen retrieval. Therefore I suggest to also use this buffer in your experiments with high temperature.

The...

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x

Es freut mich zu hören, dass Sie unser Angebot unseren Antikörper ab109025 inIHC auf gefrorenen Schnitten (IHC-Fr) zu testen, annehmen möchten. Entschuldigen Sie bitte, dass ich es nicht mehr geschafft habe, Ihnen den Code ges...

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Thank you for contacting Abcam.



The antibody ab109025 would not recognize the protein that is missing the first 250 amino acids. The peptide maps between amino acids 120 and 145 of human Prostaglandin G/H synthase 1/COX1 (UniProt: ...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Mouse Cell (mef cells)
Specification mef cells
Fixative Paraformaldehyde
Permeabilization Yes - 1% triton x100
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: rt°C
Username

Abcam user community

Verified customer

Submitted May 20 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"