Anti-COX1 / Cyclooxygenase 1 antibody [5F6/F4] (ab695)

Overview

  • Product name
    Anti-COX1 / Cyclooxygenase 1 antibody [5F6/F4]
    See all COX1 / Cyclooxygenase 1 primary antibodies
  • Description
    Mouse monoclonal [5F6/F4] to COX1 / Cyclooxygenase 1
  • Specificity
    COX-1. No cross-reactivity with Cox-2.
  • Tested applications
    Suitable for: ELISA, WB, IHC-Fr, IHC-P, Flow Cyt, IP, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    A preparation of intracellular proteins of the human cell line HL60.

  • General notes
    Please note that we have received reports from customers working with this antibody that they obtain two stong bands in Western blot. A single band at around 72kDa is expected. One customer reported that when tested with three different human cell lines two strong bands are obtained, one between 40 - 50kDa and other other between 62 - 83kDa. It is possible that a degraded form of the enzyme is detected or the bands may correspond to different glycosylation states.

Properties

Applications

Our Abpromise guarantee covers the use of ab695 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use a concentration of 0.001 - 0.01 µg/ml.
WB Use a concentration of 1 - 2 µg/ml.
IHC-Fr Use a concentration of 10 µg/ml.
IHC-P Use at an assay dependent concentration.
Flow Cyt Use 1-2µg for 106 cells.
IP Use at an assay dependent concentration.
ICC/IF Use at an assay dependent concentration.

Target

  • Function
    May play an important role in regulating or promoting cell proliferation in some normal and neoplastically transformed cells.
  • Pathway
    Lipid metabolism; prostaglandin biosynthesis.
  • Sequence similarities
    Belongs to the prostaglandin G/H synthase family.
    Contains 1 EGF-like domain.
  • Cellular localization
    Microsome membrane. Endoplasmic reticulum membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • COX 1 antibody
    • COX 3 antibody
    • COX-1 antibody
    • COX1 antibody
    • Cox3 antibody
    • Cyclooxygenase 1 antibody
    • Cyclooxygenase 3, included antibody
    • Cyclooxygenase-1 antibody
    • EC 1.14.99.1 antibody
    • Partial COX1 proteins, included antibody
    • PCOX1 antibody
    • PGG/HS antibody
    • PGH synthase 1 antibody
    • PGH1_HUMAN antibody
    • PGHS-1 antibody
    • PGHS1 antibody
    • PHS 1 antibody
    • PHS1 antibody
    • Prostaglandin G/H synthase 1 antibody
    • Prostaglandin H2 synthase 1 antibody
    • Prostaglandin-endoperoxide synthase 1 (prostaglandin G/H synthase and cyclooxygenase) antibody
    • Prostaglandin-endoperoxide synthase 1 antibody
    • PTGHS antibody
    • PTGS1 antibody
    see all

Anti-COX1 / Cyclooxygenase 1 antibody [5F6/F4] images

  • Lane 1: MM standard
    Lane 2: Cox-1 (protein standard prepared from ram seminal vesicles, supplied by Alpha Diagnostic International)
    Antibody 5F6/F4: 1 µg/ml

    Lane 1: MM standard
    Lane 2: Cox-1 (protein standard prepared from ram seminal vesicles, supplied by Alpha Diagnostic International)
    Antibody 5F6/F4: 1 µg/ml

  • ab695 at 1/400 staining human anal cancer tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed before the tissue was incubated with the antibody for 45 minutes. An HRP conjugated goat anti-mouse antibody was used as the secondary.

    See Abreview

  • Anti-COX1 / Cyclooxygenase 1 antibody [5F6/F4] (ab695) at 1 µg/ml + whole cell lysate prepared from human huh-7 cell line at 15 µg

    Secondary
    Sheep anti-mouse IgG conjugated to HRP at 1/1000 dilution

    Performed under reducing conditions.

    Observed band size : 68 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 38 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 4 minutes

    This image is courtesy of an anonymous abreview.

    Primary antibody incubated for 1 hour at 20°C in 5% milk in TBST.
    Gel running conditions: Denaturing.
    Blocked using 5% milk for 16 hours at 4°C.
    Detection method: Western lightning chemiluminescent reagent.

    See Abreview

  • Overlay histogram showing NIH3T3 cells stained with ab695 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab695, 2µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

References for Anti-COX1 / Cyclooxygenase 1 antibody [5F6/F4] (ab695)

This product has been referenced in:
  • Calvello R  et al. Highly Selective Cyclooxygenase-1 Inhibitors P6 and Mofezolac Counteract Inflammatory State both In Vitro and In Vivo Models of Neuroinflammation. Front Neurol 8:251 (2017). WB ; Mouse . Read more (PubMed: 28649222) »
  • Basu S  et al. Cellular Expression of Cyclooxygenase, Aromatase, Adipokines, Inflammation and Cell Proliferation Markers in Breast Cancer Specimen. PLoS One 10:e0138443 (2015). IHC-Fr ; Human . Read more (PubMed: 26431176) »

See all 6 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: CITRATE BUFFER
Sample
Mouse Tissue sections (MOUSE HEART)
Specification
MOUSE HEART
Permeabilization
Yes - 1% TRITON X-100
Fixative
Paraformaldehyde
Username

Abcam user community

Verified customer

Submitted Jul 02 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunoprecipitation
Sample
Human Cell lysate - whole cell (huh-7)
Total protein in input
1e+006 cells
Specification
huh-7
Treatment
cells expressing recombitant myc-tagged COX1
Immuno-precipitation step
Protein A
Username

Abcam user community

Verified customer

Submitted Jun 18 2010

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (huh-7 cell line)
Loading amount
15 µg
Specification
huh-7 cell line
Gel Running Conditions
Reduced Denaturing
Blocking step
Milk as blocking agent for 16 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Username

Abcam user community

Verified customer

Submitted Nov 27 2009

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunoprecipitation
Sample
Human Cell lysate - whole cell (293T)
Total protein in input
200 µg
Specification
293T
Immuno-precipitation step
Protein G
Username

Abcam user community

Verified customer

Submitted Dec 07 2007

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (U2OS and 293T cell line)
Loading amount
30 µg
Specification
U2OS and 293T cell line
Gel Running Conditions
Reduced Denaturing
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Username

Abcam user community

Verified customer

Submitted Nov 07 2007

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (anal cancer)
Specification
anal cancer
Fixative
Formaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrate Buffer pH 6.0
Permeabilization
No
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 22°C
Username

Abcam user community

Verified customer

Submitted Nov 02 2007

Thank you for your enquiry. I am happy to forward on the reply from the originator of the antibody, which I hope you will find helpful. "Normally IgG antibodies are purified using elution buffers at lower pH of 2-3. During this purification the...

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