Anti-COX2 / Cyclooxygenase 2 antibody [5E10/D10] (ab701)

Overview

  • Product nameAnti-COX2 / Cyclooxygenase 2 antibody [5E10/D10]
    See all COX2 / Cyclooxygenase 2 primary antibodies
  • Description
    Mouse monoclonal [5E10/D10] to COX2 / Cyclooxygenase 2
  • SpecificityCOX-2. No cross-reactivity with Cox-1.
  • Tested applicationsWB, ELISAmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    A preparation of intracellular proteins of the human cell line HL60.

  • General notesPlease note that we have received a report from a customer working with this antibody that they obtain two stong bands in WB (one between 40 - 50kDa and other other between 62 - 83kDa). A single band at around 72kDa is expected.

Applications

Our Abpromise guarantee covers the use of ab701 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.5 - 1 µg/ml.
ELISA Use a concentration of 0.1 - 0.5 µg/ml.

Target

  • FunctionMediates the formation of prostaglandins from arachidonate. May have a role as a major mediator of inflammation and/or a role for prostanoid signaling in activity-dependent plasticity.
  • PathwayLipid metabolism; prostaglandin biosynthesis.
  • Sequence similaritiesBelongs to the prostaglandin G/H synthase family.
    Contains 1 EGF-like domain.
  • Post-translational
    modifications
    S-nitrosylation by NOS2 (iNOS) activates enzme activity. S-nitrosylation may take place on different Cys residues in addition to Cys-561.
  • Cellular localizationMicrosome membrane. Endoplasmic reticulum membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • COX 2 antibody
    • COX-2 antibody
    • COX2 antibody
    • Cyclooxygenase 2 antibody
    • Cyclooxygenase 2b antibody
    • Cyclooxygenase antibody
    • Cyclooxygenase-2 antibody
    • Cyclooxygenase2 antibody
    • EC 1.14.99.1 antibody
    • fj02a10 antibody
    • Glucocorticoid-regulated inflammatory cyclooxygenase antibody
    • Glucocorticoid-regulated inflammatory Prostaglandin G/H synthase antibody
    • GRIPGHS antibody
    • hCox 2 antibody
    • Macrophage activation-associated marker protein P71/73 antibody
    • OTTHUMP00000033524 antibody
    • PES-2 antibody
    • PGG/HS antibody
    • PGH synthase 2 antibody
    • PGH2_HUMAN antibody
    • PGHS 2 antibody
    • PGHS-2 antibody
    • PGHS2 antibody
    • PHS 2 antibody
    • PHS II antibody
    • PHS2 antibody
    • Prostaglandin endoperoxide synthase 2 (prostaglandin G/H synthase and cyclooxygenase) antibody
    • Prostaglandin endoperoxide synthase 2 antibody
    • Prostaglandin G/H synthase 2 antibody
    • Prostaglandin G/H synthase 2 precursor antibody
    • Prostaglandin G/H synthase and cyclooxygenase antibody
    • Prostaglandin G/H synthase antibody
    • Prostaglandin H2 synthase 2 antibody
    • prostaglandin-endoperoxide synthase 2 (prostaglandin G/H synthase and cyclooxygenase) antibody
    • Prostaglandin-endoperoxide synthase 2 antibody
    • PTGS2 antibody
    • ptgs2a antibody
    • TIS10 antibody
    • TIS10 protein antibody
    • unp1239 antibody
    • wu:fj02a10 antibody
    see all

Anti-COX2 / Cyclooxygenase 2 antibody [5E10/D10] images

  • Lane 1: Cox-2 (protein standard prepared from ovine placenta, supplied by Alpha Diagnostic International).
    Lane 2: MM standard
    Antibody 5E10/D10: 1 µg/ml

References for Anti-COX2 / Cyclooxygenase 2 antibody [5E10/D10] (ab701)

ab701 has not yet been referenced specifically in any publications.

Product Wall

Thank you for your enquiry. We are very sorry for the trouble and for not choosing the right antibody. Please do not despair! The antibody may work in your system; it has just not been characterized for cross-reaction with rat. But it does not nece...

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We currently don't have an integrin beta 1 antibody that has been tested for application in both ELISA and Western blotting. I have contacted our accounting department and instructed them to issue you a refund for ab701. If I can be of further assistan...

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Thank you very much for the details that you have provided and for your patience. I have contacted the originator of this antibody and am waiting to hear if there have been any other complaints regarding the lot that you have received. As we have had n...

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There has been no further information for the antibody's cross reactivity with rat. We do not have another mouse COX 2 in our range. If you cannot find what you are looking for within the Abcam catalog, I suggest that you click on the link to The world...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"