Anti-COX2 / Cyclooxygenase 2 antibody (ab15191)

Sandwich ELISA - COX2 / Cyclooxygenase 2 antibody (ab15191)

Standard Curve for COX2 / Cyclooxygenase 2 (Analyte: ab58868) dilution range 1pg/ml to 1ug/ml using Capture Antibody Mouse monoclonal [AS66] to COX2 / Cyclooxygenase 2 (ab90345) at 1ug/ml and Detector Antibody Rabbit polyclonal to COX2 / Cyclooxygenase 2 (ab15191) at 0.5ug/ml

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - COX2 / Cyclooxygenase 2 antibody (ab15191)

ab15191 staining COX2 / Cyclooxygenase in human breast carcinoma by Immunohistochemistry (FFPE-sections).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - COX2 / Cyclooxygenase 2 antibody (ab15191)

ab15191 staining mouse brain sections by IHC-P.  Sections were formaldehyde fixed and subjected to heat mediated antigen retrieval in citrate buffer prior to blocking with 1% BSA for 10 minutes at RT.  The primary antibody was diluted 1/2000 and incubated with the sample for 2 hours.  A biotinylated goat anti-rabbit IgG antibody, diluted 1/300, was used as the secondary. Sections were preblocked in an Avidin-Biotin kit to mask endogenous biotin

This image is courtesy of an Abreview submitted by Mr Carl Hobbs

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - COX2 / Cyclooxygenase 2 antibody (ab15191)

ab15191 staining adult rat hippocampus tissue sections by IHC-P.  Sections were formaldehyde fixed and subjected to heat mediated antigen retrieval (in citric acid, pH6, 10mM) prior to blocking in 1% BSA for 10 minutes at RT.  The primary anitbody was diluted 1/400 and incubated with the sample for 2 hours.  A biotinylated goat anti-rabbit antibody, diluted 1/300 was used as the secondary.

Clear cytoplasmic staining in a subset of neurones in the dentate gyrus of a sagittal section of whole rat brain can be seen.  In other areas of this section, there are, additionally, small cells and their processes that are positive: they may be inter-neurones or microglia: it would be neccessary to carry out double-immunostaining to confirm this.

This image is courtesy of an Abreview submitted by Mr Carl Hobbs

Immunohistochemistry (Frozen sections) - COX2 / Cyclooxygenase 2 antibody (ab15191)

ab15191 staining tissue sections of arteriosclerotic plaque in mouse aorta by IHC-Fr.  Sections were acetone fixed and blocked in 1% serum for 10 minutes at 20°C prior to incubation with the primary antibody (diluted 1/500) for 1 hour at 20°C.  A biotinylated donkey anti-rabbit antibody (diluted 1/500) was used as the secondary.

This image is courtesy of an Abreview submitted by Miss Silke Vorwald

Western blot

All lanes : Anti-COX2 / Cyclooxygenase 2 antibody (ab15191) at 1 µg/ml

Lane 1 : COX2 / Cyclooxygenase 2 protein (His tag) (ab58868) at 0.1 µg
Lane 2 : COX2 / Cyclooxygenase 2 protein (His tag) (ab58868) at 0.01 µg

Secondary
Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP), pre-adsorbed (ab97080) at 1/5000 dilution
developed using the ECL technique

Performed under reducing conditions.

Exposure time : 10 seconds