Recombinant
RabMAb

Anti-COX2 / Cyclooxygenase 2 antibody [EPR18376-119] - C-terminal (ab188184)

Overview

  • Product name
    Anti-COX2 / Cyclooxygenase 2 antibody [EPR18376-119] - C-terminal
    See all COX2 / Cyclooxygenase 2 primary antibodies
  • Description
    Rabbit monoclonal [EPR18376-119] to COX2 / Cyclooxygenase 2 - C-terminal
  • Tested applications
    Suitable for: Flow Cyt, ICC/IF, WB, IPmore details
  • Species reactivity
    Reacts with: Mouse
  • Immunogen

    Recombinant fragment within Mouse COX2/ Cyclooxygenase 2 aa 400 to the C-terminus (C terminal). The exact sequence is proprietary.
    Database link: Q05769

  • Positive control
    • WB: NIH/3T3 whole cell lysate; RAW 264.7 treated with 1 µg/ml LPS for 6 hours whole cell lysate. ICC/IF: RAW 264.7 cells treated with 1 µg/ml LPS for 6 hours. Flow Cyt: RAW 264.7 cells treated with 1 µg/ml LPS for 6 hours. IP: NIH/3T3 whole cell lysate.
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab188184 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt 1/600.
ICC/IF 1/150.
WB 1/1000. Detects a band of approximately 74 kDa (predicted molecular weight: 69 kDa).
IP 1/30.

Target

  • Function
    Mediates the formation of prostaglandins from arachidonate. May have a role as a major mediator of inflammation and/or a role for prostanoid signaling in activity-dependent plasticity.
  • Pathway
    Lipid metabolism; prostaglandin biosynthesis.
  • Sequence similarities
    Belongs to the prostaglandin G/H synthase family.
    Contains 1 EGF-like domain.
  • Post-translational
    modifications
    S-nitrosylation by NOS2 (iNOS) activates enzme activity. S-nitrosylation may take place on different Cys residues in addition to Cys-561.
  • Cellular localization
    Microsome membrane. Endoplasmic reticulum membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • COX 2 antibody
    • COX-2 antibody
    • COX2 antibody
    • Cyclooxygenase 2 antibody
    • Cyclooxygenase 2b antibody
    • Cyclooxygenase antibody
    • Cyclooxygenase-2 antibody
    • Cyclooxygenase2 antibody
    • EC 1.14.99.1 antibody
    • fj02a10 antibody
    • Glucocorticoid-regulated inflammatory cyclooxygenase antibody
    • Glucocorticoid-regulated inflammatory Prostaglandin G/H synthase antibody
    • GRIPGHS antibody
    • hCox 2 antibody
    • Macrophage activation-associated marker protein P71/73 antibody
    • OTTHUMP00000033524 antibody
    • PES-2 antibody
    • PGG/HS antibody
    • PGH synthase 2 antibody
    • PGH2_HUMAN antibody
    • PGHS 2 antibody
    • PGHS-2 antibody
    • PGHS2 antibody
    • PHS 2 antibody
    • PHS II antibody
    • PHS2 antibody
    • Prostaglandin endoperoxide synthase 2 (prostaglandin G/H synthase and cyclooxygenase) antibody
    • Prostaglandin endoperoxide synthase 2 antibody
    • Prostaglandin G/H synthase 2 antibody
    • Prostaglandin G/H synthase 2 precursor antibody
    • Prostaglandin G/H synthase and cyclooxygenase antibody
    • Prostaglandin G/H synthase antibody
    • Prostaglandin H2 synthase 2 antibody
    • prostaglandin-endoperoxide synthase 2 (prostaglandin G/H synthase and cyclooxygenase) antibody
    • Prostaglandin-endoperoxide synthase 2 antibody
    • PTGS2 antibody
    • ptgs2a antibody
    • TIS10 antibody
    • TIS10 protein antibody
    • unp1239 antibody
    • wu:fj02a10 antibody
    see all

Images

  • Anti-COX2 / Cyclooxygenase 2 antibody [EPR18376-119] - C-terminal (ab188184) at 1/1000 dilution + NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate at 10 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
    Developed using the ECL technique

    Predicted band size : 69 kDa
    Observed band size : 74 kDa (why is the actual band size different from the predicted?)


    Exposure time : 3 seconds

    Blocking/Dilution buffer: 5% NFDM/TBST.

  • All lanes : Anti-COX2 / Cyclooxygenase 2 antibody [EPR18376-119] - C-terminal (ab188184) at 1/1000 dilution

    Lane 1 : Untreated RAW 264.7 (mouse macrophage cell line transformed with Abelson murine leukemia virus) whole cell lysate
    Lane 2 : RAW 264.7 treated with 1 µg/ml LPS for 6 hours whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
    Developed using the ECL technique

    Predicted band size : 69 kDa
    Observed band size : 74 kDa (why is the actual band size different from the predicted?)


    Exposure time : 1 second

    Blocking/Dilution buffer: 5% NFDM/TBST.

    The expression profile and molecular weight observed is consistent with what has been described in the literature (PMID: 9737714). COX2 protein expression is induced by LPS in neutrophils.

  • Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized RAW 264.7 (mouse macrophage cell line transformed with Abelson murine leukemia virus) cells, untreated or treated with lipopolysaccharide (1 µg/ml) for 6 hours, labeling COX2 / Cyclooxygenase 2 with ab188184 at 1/150 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing increased cytoplasmic staining on RAW 264.7 cells treated with lipopolysaccharides (1 µg/ml) for 6 hours.

    The nuclear counter stain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) (ab195889) (red) at 1/200 dilution.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution.

  • Flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol permeabilized RAW 264.7 (mouse macrophage cell line transformed with Abelson murine leukemia virus) cell line, treated with 1μg/ml LPS for 6h (red)  and untreated control (green), labeling COX2 / Cyclooxygenase 2 with ab188184 at 1/600 dilution compared with a Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730) (black) and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) at 1/2000 dilution was used as the secondary antibody.

  • COX2 / Cyclooxygenase 2 was immunoprecipitated from 0.35 mg of NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate with ab188184 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab188184 at 1/1000 dilution. VeriBlot for IP secondary antibody (HRP) (ab131366), was used as secondary antibody at 1/10000 dilution.

    Lane 1: NIH/3T3 whole cell lysate 10μg (Input). 

    Lane 2: ab188184 IP in NIH/3T3 whole cell lysate. 

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab188184 in NIH/3T3 whole cell lysate.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure: 30 seconds.

References

ab188184 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab188184.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up