Overview

  • Product name
    Anti-COX2 antibody [EP8588]
    See all COX2 primary antibodies
  • Description
    Rabbit monoclonal [EP8588] to COX2
  • Tested applications
    Suitable for: WB, IHC-Pmore details
    Unsuitable for: Flow Cyt,ICC/IF or IP
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Sheep, Rabbit, Horse, Cow, Mink
  • Immunogen

    Recombinant fragment within Human COX2 aa 500-600. The exact sequence is proprietary.

  • Positive control
    • A549 cell lysate; Lysate from THP1 cells treated with LPS; Human breast carcinoma and tonsil tissues.
  • General notes

    A trial size is available to purchase for this antibody.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

Properties

Applications

Our Abpromise guarantee covers the use of ab169782 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/5000. Predicted molecular weight: 69 kDa.
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
  • Application notes
    Is unsuitable for Flow Cyt,ICC/IF or IP.
  • Target

    • Function
      Mediates the formation of prostaglandins from arachidonate. May have a role as a major mediator of inflammation and/or a role for prostanoid signaling in activity-dependent plasticity.
    • Pathway
      Lipid metabolism; prostaglandin biosynthesis.
    • Sequence similarities
      Belongs to the prostaglandin G/H synthase family.
      Contains 1 EGF-like domain.
    • Post-translational
      modifications
      S-nitrosylation by NOS2 (iNOS) activates enzyme activity. S-nitrosylation may take place on different Cys residues in addition to Cys-561.
    • Cellular localization
      Microsome membrane. Endoplasmic reticulum membrane.
    • Information by UniProt
    • Database links
    • Alternative names
      • COX 2 antibody
      • COX-2 antibody
      • Cyclooxygenase 2 antibody
      • Cyclooxygenase 2b antibody
      • Cyclooxygenase-2 antibody
      • Glucocorticoid-regulated inframmatory prostagandin G/H synthase antibody
      • GRIPGHS antibody
      • hCox 2 antibody
      • PGG/HS antibody
      • PGH synthase 2 antibody
      • PGH2_HUMAN antibody
      • PGHS 2 antibody
      • PGHS-2 antibody
      • PGHS2 antibody
      • PHS 2 antibody
      • PHS II antibody
      • Prostaglandin endoperoxide synthase 2 (prostaglandin G/H synthase and cyclooxygenase) antibody
      • Prostaglandin endoperoxide synthase 2 antibody
      • Prostaglandin G/H synthase 2 antibody
      • Prostaglandin G/H synthase and cyclooxygenase antibody
      • Prostaglandin H2 synthase 2 antibody
      • Prostaglandin-endoperoxide synthase 2 antibody
      • PTGS2 antibody
      see all

    Images

    • All lanes : Anti-COX2 antibody [EP8588] (ab169782) at 1/1000 dilution

      Lane 1 : A549 cell lysate
      Lane 2 : Lysate from THP1 cells treated with LPS

      Lysates/proteins at 10 µg per lane.

      Secondary
      Goat anti-rabbit HRP at 1/2000 dilution

      Predicted band size : 69 kDa
    • Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human breast carcinoma tissue, labeling COX2 using ab169782 at a 1/50 dilution.
    • Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human tonsil tissue, labeling COX2 using ab169782 at a 1/50 dilution.

    References

    ab169782 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab169782.
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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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