Anti-COX2 / Cyclooxygenase 2 antibody (ab102005)

Overview

  • Product name
    Anti-COX2 / Cyclooxygenase 2 antibody
    See all COX2 / Cyclooxygenase 2 primary antibodies
  • Description
    Rabbit polyclonal to COX2 / Cyclooxygenase 2
  • Tested applications
    Suitable for: IHC-P, WBmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat, Sheep, Cow, Dog, Pig, Macaque monkey
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 550 to the C-terminus of Human COX2/ Cyclooxygenase 2.

  • Positive control
    • This antibody gave a positive signal in Human Lung tissue lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab102005 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 5 µg/ml.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 69 kDa (predicted molecular weight: 69 kDa).

Target

  • Function
    Mediates the formation of prostaglandins from arachidonate. May have a role as a major mediator of inflammation and/or a role for prostanoid signaling in activity-dependent plasticity.
  • Pathway
    Lipid metabolism; prostaglandin biosynthesis.
  • Sequence similarities
    Belongs to the prostaglandin G/H synthase family.
    Contains 1 EGF-like domain.
  • Post-translational
    modifications
    S-nitrosylation by NOS2 (iNOS) activates enzme activity. S-nitrosylation may take place on different Cys residues in addition to Cys-561.
  • Cellular localization
    Microsome membrane. Endoplasmic reticulum membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • COX 2 antibody
    • COX-2 antibody
    • COX2 antibody
    • Cyclooxygenase 2 antibody
    • Cyclooxygenase 2b antibody
    • Cyclooxygenase antibody
    • Cyclooxygenase-2 antibody
    • Cyclooxygenase2 antibody
    • EC 1.14.99.1 antibody
    • fj02a10 antibody
    • Glucocorticoid-regulated inflammatory cyclooxygenase antibody
    • Glucocorticoid-regulated inflammatory Prostaglandin G/H synthase antibody
    • GRIPGHS antibody
    • hCox 2 antibody
    • Macrophage activation-associated marker protein P71/73 antibody
    • OTTHUMP00000033524 antibody
    • PES-2 antibody
    • PGG/HS antibody
    • PGH synthase 2 antibody
    • PGH2_HUMAN antibody
    • PGHS 2 antibody
    • PGHS-2 antibody
    • PGHS2 antibody
    • PHS 2 antibody
    • PHS II antibody
    • PHS2 antibody
    • Prostaglandin endoperoxide synthase 2 (prostaglandin G/H synthase and cyclooxygenase) antibody
    • Prostaglandin endoperoxide synthase 2 antibody
    • Prostaglandin G/H synthase 2 antibody
    • Prostaglandin G/H synthase 2 precursor antibody
    • Prostaglandin G/H synthase and cyclooxygenase antibody
    • Prostaglandin G/H synthase antibody
    • Prostaglandin H2 synthase 2 antibody
    • prostaglandin-endoperoxide synthase 2 (prostaglandin G/H synthase and cyclooxygenase) antibody
    • Prostaglandin-endoperoxide synthase 2 antibody
    • PTGS2 antibody
    • ptgs2a antibody
    • TIS10 antibody
    • TIS10 protein antibody
    • unp1239 antibody
    • wu:fj02a10 antibody
    see all

Anti-COX2 / Cyclooxygenase 2 antibody images

  • Anti-COX2 / Cyclooxygenase 2 antibody (ab102005) at 1 µg/ml + Lung (Human) Tissue Lysate at 10 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 69 kDa
    Observed band size : 69 kDa
    Additional bands at : 43 kDa,52 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 6 minutes
  • IHC image of COX2 / Cyclooxygenase 2 staining in Human breast cancer formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab102005, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

References for Anti-COX2 / Cyclooxygenase 2 antibody (ab102005)

This product has been referenced in:
  • Kitanaka T  et al. JNK activation is essential for activation of MEK/ERK signaling in IL-1ß-induced COX-2 expression in synovial fibroblasts. Sci Rep 7:39914 (2017). WB, RT-PCR ; Cat . Read more (PubMed: 28054591) »
  • Cui TZ  et al. Modulation of the respiratory supercomplexes in yeast: enhanced formation of cytochrome oxidase increases the stability and abundance of respiratory supercomplexes. J Biol Chem 289:6133-41 (2014). WB ; Saccharomyces cerevisiae . Read more (PubMed: 24421313) »

See all 3 Publications for this product

Product Wall

Application
Western blot
Sample
Cat Cell lysate - whole cell (Synovial Fibroblasts)
Gel Running Conditions
Reduced Denaturing (12%)
Loading amount
10 µg
Treatment
100pM IL-1β for 0, 12, 24, 48 hrs
Specification
Synovial Fibroblasts
Blocking step
BlockAce as blocking agent for 50 minute(s) · Concentration: 100% · Temperature: 25°C
Username

令 中野

Verified customer

Submitted Feb 28 2017

Application
Western blot
Sample
Dog Cell lysate - whole cell (canine dermal fibroblasts)
Gel Running Conditions
Reduced Denaturing (7.5% gel)
Loading amount
10 µg
Treatment
canine recombinant IL-1β
Specification
canine dermal fibroblasts
Blocking step
Block Ace as blocking agent for 50 minute(s) · Concentration: 100%
Username

令 中野

Verified customer

Submitted Nov 12 2015

Application
Western blot
Loading amount
20 µg
Gel Running Conditions
Reduced Denaturing (10%)
Sample
Dog Cell lysate - whole cell (Canine Chondrocytes)
Specification
Canine Chondrocytes
Treatment
10ng/ml IL1B, and 100uM TNFa antagonist, for 4 hours.
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Username

Abcam user community

Verified customer

Submitted Oct 27 2014

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (pancreas)
Specification
pancreas
Fixative
Formaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Sodium Citrate
Permeabilization
No
Blocking step
GOAT BLOCK = 10mM Tris pH 7.4, 100mM MgCl2, 0.5% Tween, 1% BSA, 5% normal goat serum (IN WATER) as blocking agent for 20 minute(s) · Concentration: 100% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Apr 11 2013

Thank you for your reply.

Indeed, the other antibodies mentioned are only tested for WB so far, however, they are the antibodies against these targets that have the highest probability to cross-react with hamster due to sequence similarity o...

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Thank you very much for contacting us.

I am happy to let you know that we have the following antibodies available:

COX-2: ab102005: Click here (or use the following: http://www.abcam.com/index.html?datasheet=102005).
COX-1: a...

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