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Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) (C terminal)(Rat)
Our Abpromise guarantee covers the use of ab15191 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use at an assay dependent concentration. PubMed: 20660112|
|Sandwich ELISA||Use a concentration of 0.5 µg/ml. Can be paired for Sandwich ELISA with Mouse monoclonal [AS66] to COX2 / Cyclooxygenase 2 (ab90345). For sandwich ELISA, use this antibody as Detection at 0.5µg/ml with Ab90345 as Capture.|
|IP||Use at an assay dependent concentration.|
|IHC-P||1/200 - 1/1000.|
|WB||Use at an assay dependent concentration. Detects a band of approximately 69 kDa.|
ab15191 staining mouse brain sections by IHC-P. Sections were formaldehyde fixed and subjected to heat mediated antigen retrieval in citrate buffer prior to blocking with 1% BSA for 10 minutes at RT. The primary antibody was diluted 1/2000 and incubated with the sample for 2 hours. A biotinylated goat anti-rabbit IgG antibody, diluted 1/300, was used as the secondary. Sections were preblocked in an Avidin-Biotin kit to mask endogenous biotin
ab15191 staining COX2 in Human Colorectal Tumor tissue sections by Immunohistochemistry (IHC-Fr - frozen sections). Tissue was fixed with paraformaldehyde, permeabilized with 0.3% Triton and blocked with 5% serum for 1 hour at 24°C. Samples were incubated with primary antibody (1/500 in PBS + 0.3% Triton + 0.1% BSA) for 2 hours at 24°C. An Alexa Fluor® 488-conjugated Goat anti-rabbit polyclonal (1/1000) was used as the secondary antibody.
ab15191 staining adult rat hippocampus tissue sections by IHC-P. Sections were formaldehyde fixed and subjected to heat mediated antigen retrieval (in citric acid, pH6, 10mM) prior to blocking in 1% BSA for 10 minutes at RT. The primary anitbody was diluted 1/400 and incubated with the sample for 2 hours. A biotinylated goat anti-rabbit antibody, diluted 1/300 was used as the secondary.
Clear cytoplasmic staining in a subset of neurones in the dentate gyrus of a sagittal section of whole rat brain can be seen. In other areas of this section, there are, additionally, small cells and their processes that are positive: they may be inter-neurones or microglia: it would be neccessary to carry out double-immunostaining to confirm this.
ab15191 staining tissue sections of arteriosclerotic plaque in mouse aorta by IHC-Fr. Sections were acetone fixed and blocked in 1% serum for 10 minutes at 20°C prior to incubation with the primary antibody (diluted 1/500) for 1 hour at 20°C. A biotinylated donkey anti-rabbit antibody (diluted 1/500) was used as the secondary.
ab15191 staining COX2 / Cyclooxygenase in human breast carcinoma by Immunohistochemistry (FFPE-sections).
ab15191 staining COX2 / Cyclooxygenase 2 in Mouse liver tissue sections by IHC-Fr.
Cells were fixed with acetone and blocked with 10% Serum for 60 minutes at 22°C. Samples were incubated with primary antibody (1/200 in PBS + 0.1% Triton x-100) for 13 hour at 4°C. An Alexa Fluor®488-conjugated Goat anti-rabbit IgG polyclonal(1/1000) was used as the secondary antibody.