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Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) (C terminal)(Rat)
Our Abpromise guarantee covers the use of ab15191 in the following tested applications.
|ICC/IF||Use at an assay dependent concentration. PubMed: 20660112|
|Sandwich ELISA||Use a concentration of 0.5 µg/ml. Can be paired for Sandwich ELISA with Mouse monoclonal [AS66] to COX2 / Cyclooxygenase 2 (ab90345). For sandwich ELISA, use this antibody as Detection at 0.5µg/ml with Ab90345 as Capture.|
|IP||Use at an assay dependent concentration.|
|IHC-P||1/200 - 1/1000.|
|WB||Use at an assay dependent concentration. Detects a band of approximately 69 kDa.|
ab15191 staining mouse brain sections by IHC-P. Sections were formaldehyde fixed and subjected to heat mediated antigen retrieval in citrate buffer prior to blocking with 1% BSA for 10 minutes at RT. The primary antibody was diluted 1/2000 and incubated with the sample for 2 hours. A biotinylated goat anti-rabbit IgG antibody, diluted 1/300, was used as the secondary. Sections were preblocked in an Avidin-Biotin kit to mask endogenous biotin
ab15191 staining COX2 in Human Colorectal Tumor tissue sections by Immunohistochemistry (IHC-Fr - frozen sections). Tissue was fixed with paraformaldehyde, permeabilized with 0.3% Triton and blocked with 5% serum for 1 hour at 24°C. Samples were incubated with primary antibody (1/500 in PBS + 0.3% Triton + 0.1% BSA) for 2 hours at 24°C. An Alexa Fluor® 488-conjugated Goat anti-rabbit polyclonal (1/1000) was used as the secondary antibody.
ab15191 staining adult rat hippocampus tissue sections by IHC-P. Sections were formaldehyde fixed and subjected to heat mediated antigen retrieval (in citric acid, pH6, 10mM) prior to blocking in 1% BSA for 10 minutes at RT. The primary anitbody was diluted 1/400 and incubated with the sample for 2 hours. A biotinylated goat anti-rabbit antibody, diluted 1/300 was used as the secondary.
Clear cytoplasmic staining in a subset of neurones in the dentate gyrus of a sagittal section of whole rat brain can be seen. In other areas of this section, there are, additionally, small cells and their processes that are positive: they may be inter-neurones or microglia: it would be neccessary to carry out double-immunostaining to confirm this.
ab15191 staining tissue sections of arteriosclerotic plaque in mouse aorta by IHC-Fr. Sections were acetone fixed and blocked in 1% serum for 10 minutes at 20°C prior to incubation with the primary antibody (diluted 1/500) for 1 hour at 20°C. A biotinylated donkey anti-rabbit antibody (diluted 1/500) was used as the secondary.
ab15191 staining COX2 / Cyclooxygenase in human breast carcinoma by Immunohistochemistry (FFPE-sections).
ab15191 staining COX2 / Cyclooxygenase 2 in Mouse liver tissue sections by IHC-Fr.
Cells were fixed with acetone and blocked with 10% Serum for 60 minutes at 22°C. Samples were incubated with primary antibody (1/200 in PBS + 0.1% Triton x-100) for 13 hour at 4°C. An Alexa Fluor®488-conjugated Goat anti-rabbit IgG polyclonal(1/1000) was used as the secondary antibody.