Anti-COX2 / Cyclooxygenase 2 antibody (ab23672)
Key features and details
- Goat polyclonal to COX2 / Cyclooxygenase 2
- Suitable for: WB, IHC-P
- Reacts with: Sheep, Human
- Isotype: IgG
Get better batch-to-batch reproducibility with a recombinant antibody
- Research with confidence – consistent and reproducible results with every batch
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- Success from the first experiment – confirmed specificity through extensive validation
- Ethical standards compliant – production is animal-free
Overview
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Product name
Anti-COX2 / Cyclooxygenase 2 antibody
See all COX2 / Cyclooxygenase 2 primary antibodies -
Description
Goat polyclonal to COX2 / Cyclooxygenase 2 -
Host species
Goat -
Specificity
ab23672 recognises COX2. It does not cross react with COX1. -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Sheep, Human
Predicted to work with: Horse, Cow, Dog, Pig, Mink -
Immunogen
Synthetic peptide. This information is considered to be commercially sensitive.
(Peptide available asab49841) -
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol, PBS -
Concentration information loading...
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Purity
Protein G purified -
Purification notes
ab23672 was purified by affinity chromatography. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab23672 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB | (3) |
Use a concentration of 2 µg/ml. Predicted molecular weight: 69 kDa.
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IHC-P | (1) |
Use a concentration of 1 - 2 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Notes |
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WB
Use a concentration of 2 µg/ml. Predicted molecular weight: 69 kDa. |
IHC-P
Use a concentration of 1 - 2 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
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Function
Mediates the formation of prostaglandins from arachidonate. May have a role as a major mediator of inflammation and/or a role for prostanoid signaling in activity-dependent plasticity. -
Pathway
Lipid metabolism; prostaglandin biosynthesis. -
Sequence similarities
Belongs to the prostaglandin G/H synthase family.
Contains 1 EGF-like domain. -
Post-translational
modificationsS-nitrosylation by NOS2 (iNOS) activates enzme activity. S-nitrosylation may take place on different Cys residues in addition to Cys-561. -
Cellular localization
Microsome membrane. Endoplasmic reticulum membrane. - Information by UniProt
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Database links
- Entrez Gene: 282023 Cow
- Entrez Gene: 5743 Human
- Entrez Gene: 443460 Sheep
- Omim: 600262 Human
- SwissProt: O62698 Cow
- SwissProt: O19183 Horse
- SwissProt: P35354 Human
- SwissProt: P79208 Sheep
see all -
Alternative names
- COX 2 antibody
- COX-2 antibody
- COX2 antibody
see all
Images
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All lanes : Anti-COX2 / Cyclooxygenase 2 antibody (ab23672) at 1/500 dilution
Lane 1 : Human COX-1
Lane 2 : Recombinant ovine COX-2 protein
Lane 3 : Recombinant human COX-2 protein
Predicted band size: 69 kDa -
Immunohistochemistry analysis of formalin fixed, paraffin-embedded human kidney sections labeling COX2 / Cyclooxygenase 2 with ab23672 at 1/80 dilution. Slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase streptavidin and chromogen (DAB). Antigen retrieval was heat mediated using citrate buffer pH 6.0.
Datasheets and documents
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SDS download
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Datasheet download
References (20)
ab23672 has been referenced in 20 publications.
- Yin X et al. Celecoxib alleviates zinc deficiency-promoted colon tumorigenesis through suppressing inflammation. Aging (Albany NY) 13:8320-8334 (2021). PubMed: 33686969
- Ouyang B et al. miR-146b-5p inhibits tumorigenesis and metastasis of gallbladder cancer by targeting Toll-like receptor 4 via the nuclear factor-?B pathway. Oncol Rep 45:N/A (2021). PubMed: 33649824
- Zhao Z et al. Fecal microbiota transplantation protects rotenone-induced Parkinson's disease mice via suppressing inflammation mediated by the lipopolysaccharide-TLR4 signaling pathway through the microbiota-gut-brain axis. Microbiome 9:226 (2021). PubMed: 34784980
- Litvin DG et al. Peripheral-to-central immune communication at the area postrema glial-barrier following bleomycin-induced sterile lung injury in adult rats. Brain Behav Immun 87:610-633 (2020). PubMed: 32097765
- Pan Y et al. Estrogen improves the development of yak (Bos grunniens) oocytes by targeting cumulus expansion and levels of oocyte-secreted factors during in vitro maturation. PLoS One 15:e0239151 (2020). PubMed: 32941516