Overview

  • Product nameAnti-COX2 / Cyclooxygenase 2 antibody
    See all COX2 / Cyclooxygenase 2 primary antibodies
  • Description
    Rabbit polyclonal to COX2 / Cyclooxygenase 2
  • Tested applicationsSuitable for: ELISA, WB, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide corresponding to Human COX2/ Cyclooxygenase 2.

  • Positive control
    • Extracts from A549 cells. This antibody gave a positive result in IHC in the following FFPE tissue: Human lung adenocarcinoma.

Applications

Our Abpromise guarantee covers the use of ab52237 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/20000.
WB 1/300 - 1/1000. Detects a band of approximately 69 kDa (predicted molecular weight: 69 kDa).
IHC-P Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF 1/100.

Target

  • FunctionMediates the formation of prostaglandins from arachidonate. May have a role as a major mediator of inflammation and/or a role for prostanoid signaling in activity-dependent plasticity.
  • PathwayLipid metabolism; prostaglandin biosynthesis.
  • Sequence similaritiesBelongs to the prostaglandin G/H synthase family.
    Contains 1 EGF-like domain.
  • Post-translational
    modifications
    S-nitrosylation by NOS2 (iNOS) activates enzme activity. S-nitrosylation may take place on different Cys residues in addition to Cys-561.
  • Cellular localizationMicrosome membrane. Endoplasmic reticulum membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • COX 2 antibody
    • COX-2 antibody
    • COX2 antibody
    • Cyclooxygenase 2 antibody
    • Cyclooxygenase 2b antibody
    • Cyclooxygenase antibody
    • Cyclooxygenase-2 antibody
    • Cyclooxygenase2 antibody
    • EC 1.14.99.1 antibody
    • fj02a10 antibody
    • Glucocorticoid-regulated inflammatory cyclooxygenase antibody
    • Glucocorticoid-regulated inflammatory Prostaglandin G/H synthase antibody
    • GRIPGHS antibody
    • hCox 2 antibody
    • Macrophage activation-associated marker protein P71/73 antibody
    • OTTHUMP00000033524 antibody
    • PES-2 antibody
    • PGG/HS antibody
    • PGH synthase 2 antibody
    • PGH2_HUMAN antibody
    • PGHS 2 antibody
    • PGHS-2 antibody
    • PGHS2 antibody
    • PHS 2 antibody
    • PHS II antibody
    • PHS2 antibody
    • Prostaglandin endoperoxide synthase 2 (prostaglandin G/H synthase and cyclooxygenase) antibody
    • Prostaglandin endoperoxide synthase 2 antibody
    • Prostaglandin G/H synthase 2 antibody
    • Prostaglandin G/H synthase 2 precursor antibody
    • Prostaglandin G/H synthase and cyclooxygenase antibody
    • Prostaglandin G/H synthase antibody
    • Prostaglandin H2 synthase 2 antibody
    • prostaglandin-endoperoxide synthase 2 (prostaglandin G/H synthase and cyclooxygenase) antibody
    • Prostaglandin-endoperoxide synthase 2 antibody
    • PTGS2 antibody
    • ptgs2a antibody
    • TIS10 antibody
    • TIS10 protein antibody
    • unp1239 antibody
    • wu:fj02a10 antibody
    see all

Anti-COX2 / Cyclooxygenase 2 antibody images

  • All lanes : Anti-COX2 / Cyclooxygenase 2 antibody (ab52237) at 1/500 dilution

    Lane 1 : A549 (Human lung adenocarcinoma epithelial)
    Lane 2 : A549 (Human lung adenocarcinoma epithelial) with Synthesized Peptide


    Predicted band size : 69 kDa
  • IHC image of COX2 / Cyclooxygenase 2 staining in Human lung adenocarcinoma formalin fixed paraffin embedded tissue section, performed on a Leica Bond™ system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab52237, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

  • Immunocytochemistry/immunofluorescence analysis of A549 (Human lung adenocarcinoma epithelial) cells labelling COX2 / Cyclooxygenase 2 with ab52237 at dilution of 1/100. No staining was abserved when blocked with synthesized peptide as shown in the inset.

  • Anti-COX2 / Cyclooxygenase 2 antibody (ab52237) (Human mesothelioma whole cell lysate.)

    Secondary
    Goat anti-rabbit HRP

    Predicted band size : 69 kDa

    This image is courtesy of an anonymous Abreview

    Please see accompanying abreview for additional information

    See Abreview

References for Anti-COX2 / Cyclooxygenase 2 antibody (ab52237)

This product has been referenced in:
  • Riemann A  et al. Acidic environment activates inflammatory programs in fibroblasts via a cAMP-MAPK pathway. Biochim Biophys Acta 1853:299-307 (2015). Read more (PubMed: 25461841) »
  • Endo Y  et al. Differences in PGE2 production between primary human monocytes and differentiated macrophages: role of IL-1ß and TRIF/IRF3. PLoS One 9:e98517 (2014). WB ; Human . Read more (PubMed: 24870145) »

See all 9 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (primary myometrial cells)
Loading amount 20 µg
Specification primary myometrial cells
Treatment not serum starved
Gel Running Conditions Reduced Denaturing (8% Bis-Tris)
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Username

Abcam user community

Verified customer

Submitted Oct 09 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Rat Tissue lysate - whole (Lung)
Loading amount 30 µg
Specification Lung
Gel Running Conditions Reduced Denaturing (12%)
Blocking step BSA as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: 20°C
Username

Mr. Weibin Fang

Verified customer

Submitted Feb 10 2010

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (epithelial)
Loading amount 25 µg
Specification epithelial
Gel Running Conditions Non-reduced Denaturing (10%)
Blocking step Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Aug 05 2009

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (Mesothelioma)
Loading amount 20 µg
Specification Mesothelioma
Gel Running Conditions Non-reduced Denaturing (12% Tris-HEPES-SDS)
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Username

Abcam user community

Verified customer

Submitted Mar 06 2009

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"