Anti-COX2 / Cyclooxygenase 2 antibody (ab81354)

Overview

  • Product nameAnti-COX2 / Cyclooxygenase 2 antibody
    See all COX2 / Cyclooxygenase 2 primary antibodies
  • Description
    Rabbit polyclonal to COX2 / Cyclooxygenase 2
  • Tested applicationsSuitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    A synthetic peptide corresponding to a sequence at the N-terminal of Human COX2/ Cyclooxygenase 2, different to the related rat sequence by two amino acids.

Properties

Applications

Our Abpromise guarantee covers the use of ab81354 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 69 kDa.
IHC-P Use a concentration of 1 - 2 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Target

  • FunctionMediates the formation of prostaglandins from arachidonate. May have a role as a major mediator of inflammation and/or a role for prostanoid signaling in activity-dependent plasticity.
  • PathwayLipid metabolism; prostaglandin biosynthesis.
  • Sequence similaritiesBelongs to the prostaglandin G/H synthase family.
    Contains 1 EGF-like domain.
  • Post-translational
    modifications
    S-nitrosylation by NOS2 (iNOS) activates enzme activity. S-nitrosylation may take place on different Cys residues in addition to Cys-561.
  • Cellular localizationMicrosome membrane. Endoplasmic reticulum membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • COX 2 antibody
    • COX-2 antibody
    • COX2 antibody
    • Cyclooxygenase 2 antibody
    • Cyclooxygenase 2b antibody
    • Cyclooxygenase antibody
    • Cyclooxygenase-2 antibody
    • Cyclooxygenase2 antibody
    • EC 1.14.99.1 antibody
    • fj02a10 antibody
    • Glucocorticoid-regulated inflammatory cyclooxygenase antibody
    • Glucocorticoid-regulated inflammatory Prostaglandin G/H synthase antibody
    • GRIPGHS antibody
    • hCox 2 antibody
    • Macrophage activation-associated marker protein P71/73 antibody
    • OTTHUMP00000033524 antibody
    • PES-2 antibody
    • PGG/HS antibody
    • PGH synthase 2 antibody
    • PGH2_HUMAN antibody
    • PGHS 2 antibody
    • PGHS-2 antibody
    • PGHS2 antibody
    • PHS 2 antibody
    • PHS II antibody
    • PHS2 antibody
    • Prostaglandin endoperoxide synthase 2 (prostaglandin G/H synthase and cyclooxygenase) antibody
    • Prostaglandin endoperoxide synthase 2 antibody
    • Prostaglandin G/H synthase 2 antibody
    • Prostaglandin G/H synthase 2 precursor antibody
    • Prostaglandin G/H synthase and cyclooxygenase antibody
    • Prostaglandin G/H synthase antibody
    • Prostaglandin H2 synthase 2 antibody
    • prostaglandin-endoperoxide synthase 2 (prostaglandin G/H synthase and cyclooxygenase) antibody
    • Prostaglandin-endoperoxide synthase 2 antibody
    • PTGS2 antibody
    • ptgs2a antibody
    • TIS10 antibody
    • TIS10 protein antibody
    • unp1239 antibody
    • wu:fj02a10 antibody
    see all

Anti-COX2 / Cyclooxygenase 2 antibody images

  • ab81354 at 1µg/ml staining cox2 / Cyclooxygenase 2 in Human mammary cancer tissue sections by Immunohistochemistry (Formalin/ PFA-fixed paraffin-embedded sections). A Biotin-conjugated Goat anti-rabbit IgG was used as secondary at 1/200 dilution.

  • All lanes : Anti-COX2 / Cyclooxygenase 2 antibody (ab81354) at 1 µg/ml

    Lane 1 : Whole cell lysates prepared from MCF7 cells.
    Lane 2 : Whole cell lysates prepared from Hela cells.
    Lane 3 : Whole cell lysates prepared from MM453 cells.
    Lane 4 : Whole cell lysates prepared from Jurkat cell.
    Lane 5 : Whole cell lysates prepared from colo320 cells.

    Lysates/proteins at 50 µg per lane.

    Secondary
    HRP-conjugated Goat anti-rabbit IgG at 1/3000 dilution

    Predicted band size : 69 kDa

References for Anti-COX2 / Cyclooxygenase 2 antibody (ab81354)

ab81354 has not yet been referenced specifically in any publications.

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