Anti-COX2 / Cyclooxygenase 2 antibody [EP1978Y] (ab62331)

Overview

  • Product nameAnti-COX2 / Cyclooxygenase 2 antibody [EP1978Y]
    See all COX2 / Cyclooxygenase 2 primary antibodies
  • Description
    Rabbit monoclonal [EP1978Y] to COX2 / Cyclooxygenase 2
  • Tested applicationsSuitable for: ICC/IF, Flow Cyt, WBmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to Human COX2/ Cyclooxygenase 2 aa 100-200.
    Database link: P35354

  • Positive control
    • Raw264.7 cell lysate + LPS. FC: HeLa cells
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

     

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated ‘PUR’ on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

    Alternative versions available:

    Anti-COX2 / Cyclooxygenase 2 antibody (Alexa Fluor® 594) [EP1978Y] (ab202266)

    Anti-COX2 / Cyclooxygenase 2 antibody (Alexa Fluor® 488) [EP1978Y] (ab200070)

Applications

Our Abpromise guarantee covers the use of ab62331 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/100 - 1/250.
Flow Cyt 1/80.
WB 1/500 - 1/1000. Detects a band of approximately 72 kDa (predicted molecular weight: 69 kDa).Can be blocked with COX2 / Cyclooxygenase 2 peptide (ab213704).

Target

  • FunctionMediates the formation of prostaglandins from arachidonate. May have a role as a major mediator of inflammation and/or a role for prostanoid signaling in activity-dependent plasticity.
  • PathwayLipid metabolism; prostaglandin biosynthesis.
  • Sequence similaritiesBelongs to the prostaglandin G/H synthase family.
    Contains 1 EGF-like domain.
  • Post-translational
    modifications
    S-nitrosylation by NOS2 (iNOS) activates enzme activity. S-nitrosylation may take place on different Cys residues in addition to Cys-561.
  • Cellular localizationMicrosome membrane. Endoplasmic reticulum membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • COX 2 antibody
    • COX-2 antibody
    • COX2 antibody
    • Cyclooxygenase 2 antibody
    • Cyclooxygenase 2b antibody
    • Cyclooxygenase antibody
    • Cyclooxygenase-2 antibody
    • Cyclooxygenase2 antibody
    • EC 1.14.99.1 antibody
    • fj02a10 antibody
    • Glucocorticoid-regulated inflammatory cyclooxygenase antibody
    • Glucocorticoid-regulated inflammatory Prostaglandin G/H synthase antibody
    • GRIPGHS antibody
    • hCox 2 antibody
    • Macrophage activation-associated marker protein P71/73 antibody
    • OTTHUMP00000033524 antibody
    • PES-2 antibody
    • PGG/HS antibody
    • PGH synthase 2 antibody
    • PGH2_HUMAN antibody
    • PGHS 2 antibody
    • PGHS-2 antibody
    • PGHS2 antibody
    • PHS 2 antibody
    • PHS II antibody
    • PHS2 antibody
    • Prostaglandin endoperoxide synthase 2 (prostaglandin G/H synthase and cyclooxygenase) antibody
    • Prostaglandin endoperoxide synthase 2 antibody
    • Prostaglandin G/H synthase 2 antibody
    • Prostaglandin G/H synthase 2 precursor antibody
    • Prostaglandin G/H synthase and cyclooxygenase antibody
    • Prostaglandin G/H synthase antibody
    • Prostaglandin H2 synthase 2 antibody
    • prostaglandin-endoperoxide synthase 2 (prostaglandin G/H synthase and cyclooxygenase) antibody
    • Prostaglandin-endoperoxide synthase 2 antibody
    • PTGS2 antibody
    • ptgs2a antibody
    • TIS10 antibody
    • TIS10 protein antibody
    • unp1239 antibody
    • wu:fj02a10 antibody
    see all

Anti-COX2 / Cyclooxygenase 2 antibody [EP1978Y] images

  • All lanes : Anti-COX2 / Cyclooxygenase 2 antibody [EP1978Y] (ab62331) at 1/1000 dilution (purified)

    Lane 1 : untreated Raw 264.7 cell lysate
    Lane 2 : RAW264.7 treated with LPS

    Lysates/proteins at 10 µg per lane.

    Secondary
    HRP goat anti-rabbit IgG (H+L) at 1/50000 dilution

    Predicted band size : 69 kDa
    Observed band size : 72 kDa (why is the actual band size different from the predicted?)

    Blocking buffer: 5% NFDM/TBST
    Dilution buffer: 5% NFDM/TBST

  • Immunofluorescence staining of RAW264.7 cells with purified ab62331 at a working dilution of 1/200, counter-stained with DAPI. The secondary antibody was Alexa Fluor® 488 goat anti-rabbit (ab150077), used at a dilution of 1/1000. ab7291, a mouse anti-tubulin antibody (1/1000), was used to stain tubulin along with ab150120 (Alexa Fluor® 594 goat anti-mouse, 1/1000), shown in the top right hand panel. The cells were fixed in 100% methanol and permeabilized using 0.1% Triton X 100. The negative controls are shown in bottom middle and right hand panels - for negative control 1, purified ab62331 was used at a dilution of 1/500 followed by an Alexa Fluor® 594 goat anti-mouse antibody (ab150120) at a dilution of 1/500. For negative control 2, ab7291 (mouse anti-tubulin) was used at a dilution of 1/500 followed by an Alexa Fluor® 488 goat anti-rabbit antibody (ab150077) at a dilution of 1/400.

  • Unpurified ab62331 staining COX2 / Cyclooxygenase 2 in Mouse 14.5 dpc tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde, permeabilized with TBS-T and blocked with 1% BSA + 1% FBS in TBS for 2 hours at room temperature; antigen retrieval was by heat mediation in Tris buffer, pH9. Samples were incubated with primary antibody (1/100 in blocking buffer) for 16 hours. An undiluted HRP-conjugated Goat anti-rabbit IgG polyclonal was used as the secondary antibody.

    See Abreview

  • Flow Cytometry analysis of HeLa (human cervix adenocarcinoma) cells labelling COX2 / Cyclooxygenase 2 with purified ab62331 at 1/80 (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. An Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/2000) was used as the secondary antibody. Black - Isotype control, rabbit monoclonal IgG. Blue - Unlabelled control, cells without incubation with primary and secondary antibodies.

  • Unpurified ab62331 staining HeLa cells by ICC/IF. The cells were fixed with paraformaldehyde and permeabilized with 0.5% Triton X100 in PBS. The cells were then stained with ab62331 at 1/200 in PBS for 1h at 22°C. An Alexa Fluro 488 goat anti-rabbit polyclonal antibody at 1/200 was used as the secondary antibody. Nuclei are stained in red with DAPI. ab62331 produces the expected cytoplasmic staining pattern.

    See Abreview

  • Anti-COX2 / Cyclooxygenase 2 antibody [EP1978Y] (ab62331) at 1/500 dilution (unpurified) + Raw264.7 cell lysate (10ug) + LPS

    Secondary
    Goat anti-Rabbit HRP conjugated at 2000

    Predicted band size : 69 kDa
    Observed band size : 72 kDa (why is the actual band size different from the predicted?)

References for Anti-COX2 / Cyclooxygenase 2 antibody [EP1978Y] (ab62331)

This product has been referenced in:
  • Khalesi M  et al. Comparison of PTCH1, COX-2, p53, and Ki-67 protein expression in basal cell carcinomas of nodular and superficial subtypes arising on the head and trunk. Int J Dermatol 55:1096-105 (2016). Read more (PubMed: 27126210) »
  • Hsu CK  et al. Sphingosine-1-phosphate mediates COX-2 expression and PGE2 /IL-6 secretion via c-Src-dependent AP-1 activation. J Cell Physiol 230:702-15 (2015). Read more (PubMed: 25201048) »

See all 7 Publications for this product

Product Wall

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step Serum as blocking agent for 3 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: RT°C
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Tris EDTA pH 9
Sample Dog Tissue sections (MDCK pellets in paraffin)
Specification MDCK pellets in paraffin
Permeabilization Yes - Tween-20
Fixative Paraformaldehyde
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Submitted Jan 05 2015

Application Immunocytochemistry/ Immunofluorescence
Blocking step BSA as blocking agent for 3 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: RT°C
Sample Mouse Cell (L cells)
Specification L cells
Permeabilization Yes - Triton 1%
Fixative Paraformaldehyde
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Submitted Dec 10 2014

Application Immunocytochemistry/ Immunofluorescence
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 1°C
Sample Human Cell (293T)
Specification 293T
Permeabilization Yes - Triton 1%
Fixative Paraformaldehyde
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Submitted Nov 20 2014

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step FBS / BSA as blocking agent for 3 hour(s) and 0 minute(s) · Concentration: 1%
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Tris pH 9
Sample Human Tissue sections (Infantile fibromatosis)
Specification Infantile fibromatosis
Permeabilization No
Fixative Formaldehyde
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Submitted Jul 04 2014

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step 1%BSA / 1% FBS in TBS as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: RT°C
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Tris buffer pH 9
Sample Mouse Tissue sections (Mouse 14.5 dpc)
Specification Mouse 14.5 dpc
Permeabilization Yes - TBS-T
Fixative Formaldehyde
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Submitted Sep 25 2013

Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (HeLa)
Specification HeLa
Permeabilization Yes - 0.5% Triton X100 in PBS
Fixative Paraformaldehyde
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Dr. Kirk McManus

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Submitted Aug 29 2013

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Mouse Tissue sections (pancreas)
Specification pancreas
Fixative Formaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Sodium Citrate
Permeabilization No
Blocking step GOAT BLOCK = 10mM Tris pH 7.4, 100mM MgCl2, 0.5% Tween, 1% BSA, 5% normal goat serum (IN WATER) as blocking agent for 20 minute(s) · Concentration: 100% · Temperature: 25°C
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Submitted Apr 11 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Mouse Cell lysate - whole cell (mMSC)
Loading amount 30 µg
Specification mMSC
Gel Running Conditions Reduced Denaturing (12)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: RT°C
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Submitted Jan 21 2013

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"