Anti-COX2 / Cyclooxygenase 2 antibody [SP21] (ab16701)

Overview

  • Product name
    Anti-COX2 / Cyclooxygenase 2 antibody [SP21]
    See all COX2 / Cyclooxygenase 2 primary antibodies
  • Description
    Rabbit monoclonal [SP21] to COX2 / Cyclooxygenase 2
  • Tested applications
    Suitable for: IHC-Pmore details
    Unsuitable for: WB
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic peptide (Rat) (C terminal).

  • Positive control
    • Lung carcinoma, colon carcinoma and breast carcinoma; HT 29 lysate.

Applications

Our Abpromise guarantee covers the use of ab16701 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
  • Application notes
    Is unsuitable for WB.
  • Target

    • Function
      Mediates the formation of prostaglandins from arachidonate. May have a role as a major mediator of inflammation and/or a role for prostanoid signaling in activity-dependent plasticity.
    • Pathway
      Lipid metabolism; prostaglandin biosynthesis.
    • Sequence similarities
      Belongs to the prostaglandin G/H synthase family.
      Contains 1 EGF-like domain.
    • Post-translational
      modifications
      S-nitrosylation by NOS2 (iNOS) activates enzme activity. S-nitrosylation may take place on different Cys residues in addition to Cys-561.
    • Cellular localization
      Microsome membrane. Endoplasmic reticulum membrane.
    • Information by UniProt
    • Database links
    • Alternative names
      • COX 2 antibody
      • COX-2 antibody
      • COX2 antibody
      • Cyclooxygenase 2 antibody
      • Cyclooxygenase 2b antibody
      • Cyclooxygenase antibody
      • Cyclooxygenase-2 antibody
      • Cyclooxygenase2 antibody
      • EC 1.14.99.1 antibody
      • fj02a10 antibody
      • Glucocorticoid-regulated inflammatory cyclooxygenase antibody
      • Glucocorticoid-regulated inflammatory Prostaglandin G/H synthase antibody
      • GRIPGHS antibody
      • hCox 2 antibody
      • Macrophage activation-associated marker protein P71/73 antibody
      • OTTHUMP00000033524 antibody
      • PES-2 antibody
      • PGG/HS antibody
      • PGH synthase 2 antibody
      • PGH2_HUMAN antibody
      • PGHS 2 antibody
      • PGHS-2 antibody
      • PGHS2 antibody
      • PHS 2 antibody
      • PHS II antibody
      • PHS2 antibody
      • Prostaglandin endoperoxide synthase 2 (prostaglandin G/H synthase and cyclooxygenase) antibody
      • Prostaglandin endoperoxide synthase 2 antibody
      • Prostaglandin G/H synthase 2 antibody
      • Prostaglandin G/H synthase 2 precursor antibody
      • Prostaglandin G/H synthase and cyclooxygenase antibody
      • Prostaglandin G/H synthase antibody
      • Prostaglandin H2 synthase 2 antibody
      • prostaglandin-endoperoxide synthase 2 (prostaglandin G/H synthase and cyclooxygenase) antibody
      • Prostaglandin-endoperoxide synthase 2 antibody
      • PTGS2 antibody
      • ptgs2a antibody
      • TIS10 antibody
      • TIS10 protein antibody
      • unp1239 antibody
      • wu:fj02a10 antibody
      see all

    Images

    • ab16701 at 1/100 dilution staining COX2 / Cyclooxygenase 2 in human breast carcinoma by Immunohistochemistry (FFPE-sections).

    References

    This product has been referenced in:
    • Szylberg L  et al. Expression of COX-2, IL-1ß, TNF-a and IL-4 in epithelium of serrated adenoma, adenoma and hyperplastic polyp. Arch Med Sci 12:172-8 (2016). Read more (PubMed: 26925134) »
    • Fujita M  et al. COX-2 blockade suppresses gliomagenesis by inhibiting myeloid-derived suppressor cells. Cancer Res 71:2664-74 (2011). Read more (PubMed: 21324923) »

    See all 3 Publications for this product

    Customer reviews and Q&As

    Unfortunately it is not possible to determine the concentration since tissue culture supernatant will contain many other host proteins besides the antibody of interest as it is unpurified. As a rough guide tissue culture supernatant contains 1 mg/ml to...

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    I'm very sorry to hear you are experiencing problems with ab16701. The protocol you have used is very good and should work well; in light of your problems it is clear that the antibody did not work well in western blotting. I would like to offer ...

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    Thank you for your enquiry. I am not sure that I follow your question, please can you elaborate. ab16701, anti-COX2 recognizes both the rat COX2 (69164KDa) and the human COX2 (68996KDa). I hope this information helps. Please do not hesitate...

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    Our source for this antibody has recommended HT 29 lysate as a positive control for Western blotting. This type of lysate can be obtained from Abcam; it is catalog # ab3952. If you have any additional questions, please contact us again.

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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