Anti-COX4 + COX4L2 antibody [10G8D12C12] (ab110261)

Abreviews (2)Q&A (4)Specific References (35)

Overview

  • Product name
    Anti-COX4 + COX4L2 antibody [10G8D12C12]
    See all COX4 + COX4L2 primary antibodies
  • Description
    Mouse monoclonal [10G8D12C12] to COX4 + COX4L2
  • Host species
    Mouse
  • Tested applications
    Suitable for: IHC-Fr, WB, Flow Cyt, ICC/IF, IHC-Pmore details
  • Species reactivity
    Reacts with: Cow, Human, Zebrafish
  • Immunogen

    Full length native Bovine protein, COX4 + COX4L2.

  • Positive control
    • Isolated mitochondria from Human heart, Bovine heart and HepG2 cells. Human skeletal muscle (frozen sections). Cultured Human embryonic lung derived fibroblasts (strain MRC5; fixed and permeabilized). Human colon tissue sections. HeLa cells.
  • General notes

    This antibody clone is manufactured by Abcam.

    Product was previously marketed under the MitoSciences sub-brand.

    If you require this antibody in a particular buffer formulation or a particular conjugate for your experiments, please contact orders@abcam.com or you can find further information here.

Properties

Applications

Our Abpromise guarantee covers the use of ab110261 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr 1/800.
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 20 kDa.
Flow Cyt Use a concentration of 1 µg/ml.

ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.
ICC/IF Use a concentration of 5 µg/ml. (heat-induced antigen-retrieval improves signal)
IHC-P 1/200.

Target

  • Relevance
    Cytochrome C Oxidase is located in the inner mitochondrial membrane and is the terminal enzyme complex of the mitochondrial electron transport chain. It collects electrons that are transferred from reduced cytochrome C and donates them to molecular oxygen, which is then reduced to water. It is composed of cytochrome A and cytochrome B, two copper atoms, and 13 different protein subunits, three of which are encoded by the mitochondrial DNA and ten others by nuclear DNA (mammals).
  • Cellular localization
    Mitochondrial inner membrane
  • Database links
    • Alternative names
      • AL024441 antibody
      • COX 4 antibody
      • COX IV antibody
      • Cox4 antibody
      • Cox4a antibody
      • COX4B antibody
      • COX4I1 antibody
      • COX4I2 antibody
      • COX4L2 antibody
      • COXIV antibody
      • Cytochrome c oxidase polypeptide IV antibody
      • Cytochrome c oxidase subunit 4 isoform 1 mitochondrial antibody
      • Cytochrome C Oxidase subunit IV antibody
      • Cytochrome c oxidase subunit IV isoform 1 antibody
      • Cytochrome c oxidase subunit IV isoform 2 (lung) antibody
      • Cytochrome c oxydase subunit 4 antibody
      • dJ857M17.2 antibody
      • MGC105470 antibody
      • MGC72016 antibody
      see all

    Images

    • Western blot - Anti-COX4 + COX4L2 antibody [10G8D12C12] (ab110261)
      Western blot - Anti-COX4 + COX4L2 antibody [10G8D12C12] (ab110261)
      All lanes : Anti-COX4 + COX4L2 antibody [10G8D12C12] (ab110261) at 1 µg/ml

      Lane 1 : Isolated mitochondria from Human heart at 5 µg
      Lane 2 : Isolated mitochondria from Bovine heart at 1 µg
      Lane 3 : Isolated mitochondria from HepG2 at 20 µg

      Predicted band size: 20 kDa

    • Flow Cytometry - Anti-COX4 + COX4L2 antibody [10G8D12C12] (ab110261)
      Flow Cytometry - Anti-COX4 + COX4L2 antibody [10G8D12C12] (ab110261)
      ab110261, at 1 µg/mL, staining COX4 + COX4L2 in HeLa cells by Immunohistochemistry (blue) and an isotype control antibody, at 1 µg/mL, staining of HeLa cells (red).
    • Immunocytochemistry/ Immunofluorescence - Anti-COX4 + COX4L2 antibody [10G8D12C12] (ab110261)
      Immunocytochemistry/ Immunofluorescence - Anti-COX4 + COX4L2 antibody [10G8D12C12] (ab110261)
      ab110261, at 10 µg/ml, staining COX4 + COX4L2 in cultured Human embryonic lung derived fibroblasts by Immunofluorescence. The cells (strain MRC5) were fixed, permeabilized and then labeled with ab110261 followed by an AlexaFluor® 488 conjugated Goat anti-mouse IgG2a isotype specific secondary antibody at 1 µg/ml.
    • Immunohistochemistry (Frozen sections) - Anti-COX4 + COX4L2 antibody [10G8D12C12] (ab110261)
      Immunohistochemistry (Frozen sections) - Anti-COX4 + COX4L2 antibody [10G8D12C12] (ab110261)
      ab110261, at 1/800, staining COX4 + COX4L2 in skeletal muscle tissue sections from a patient with a single large deletion of the mtDNA by Immunohistochemistry. The tissue was snap frozen and fixed in 4% PFA. Image kindly provided by Dr. J. Murphy and D. Turnbull, Mitochondrial Research Group, Newcastle University.
    • Immunohistochemistry (Frozen sections) - Anti-COX4 + COX4L2 antibody [10G8D12C12] (ab110261)
      Immunohistochemistry (Frozen sections) - Anti-COX4 + COX4L2 antibody [10G8D12C12] (ab110261)
      ab110261, at 1/200, showing COX4 + COX4L2 negative crypts in colon tissue sections from a normal ageing patient by Immunohistochemistry. The tissue was snap frozen and fixed in 4% PFA. Image kindly provided by Dr. J. Murphy and D. Turnbull, Mitochondrial Research Group, Newcastle University.

    References

    This product has been referenced in:
    • Baertling F  et al. NDUFA9 point mutations cause a variable mitochondrial complex I assembly defect. Clin Genet 93:111-118 (2018). WB ; Human . Read more (PubMed: 28671271) »
    • Russell OM  et al. Preferential amplification of a human mitochondrial DNA deletion in vitro and in vivo. Sci Rep 8:1799 (2018). Read more (PubMed: 29379065) »

    See all 35 Publications for this product

    Publishing research using ab110261? Please let us know so that we can cite the reference in this datasheet.

    Customer reviews and Q&As

    Submit a review Submit a question

    6 reviews or Q&A

    10
    Votes: Highest First

    thank you very much for your prompt reply.
    I am referring to yhe antibody ab 110261.
    Best regards,

    Thank you for confirming the product code number.

    This antibody has been tested for immunohistochemistry by one of our collaborators. The optimal dilution varies depending on tissue types but 5ug/mL is recommended as a good starting point. Read More

    I need the MSDS for the following products ordered today with order number:

    Anti-GRIM19 antibody [6E1BH7] (ab110240)
    Anti-ATPB antibody [3D5] - Mitochondrial Marker (ab14730)
    Anti-COX4 + COX4L2 antibody [10G8D12C12 ] (ab110261)
    A...

    Read More

    Thank you for contacting us.

    The MSDS for these products have been added to each product datasheet. They should appear on the website within the next few hours (time for the cache to be regenerated).

    I hope this information is helpful...

    Read More

    Klone heißen anders/kürzer:
    ab14744 : 20E8
    ab14705 : 1D6E
    Kundin hat diese AKs am 10.1.2011 bestellt, kamen als Lyophilisat und wurden eingefroren bei -20C
    Ist das okay?
    Sind die Klone dieselben?

    Vielen Dank für Ihren Anruf.

    Ich freue mich Ihnen bestätigen zu können, dass die Klone dieselben sind und früher bei MitoScience nur abgekürzt wurden.

    Ich kann Ihnen auch bestätigen, dass eine Lagerung ...

    Read More

    Western blot abreview for Anti-COX4 + COX4L2 antibody [10G8D12C12 ]

     Poor
    Abreviews
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell
    Loading amount
    50 µg
    Gel Running Conditions
    Reduced Denaturing (Reduced Denaturing (Reduced Denaturing (4-15%))
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
    Read More
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    Username

    Abcam user community

    Verified customer

    Submitted Oct 09 2012

    Kindly advise if ab110261, ab 63947 will cross react with mouse samples (for WB).

    Thank you for contacting us.
    Unfortunately, ab110261 has been tested and found to be unsuitable for use on mouse samples, and ab63947 was raised against a very short immunogen that shows only 69% homology with mouse. We would not expect either of ...

    Read More

    Western blot abreview for Anti-COX4 + COX4L2 antibody [10G8D12C12 ]

     Good
    Abreviews
    Application
    Western blot
    Sample
    Mouse Cell lysate - other (Isolated Mouse Liver Mitochondria)
    Loading amount
    20 µg
    Specification
    Isolated Mouse Liver Mitochondria
    Gel Running Conditions
    Reduced Denaturing (10% Bis Tris)
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Read More
    abreview image
    Username

    Abcam user community

    Verified customer

    Submitted Dec 05 2011

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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