Anti-COX4 + COX4L2 antibody [10G8D12C12] (ab110261)
- Datasheet
- Specific References (30)
- Protocols
Overview
- Product nameAnti-COX4 + COX4L2 antibody [10G8D12C12]
See all COX4 + COX4L2 primary antibodies - DescriptionMouse monoclonal [10G8D12C12] to COX4 + COX4L2
- Tested applicationsSuitable for: IHC-Fr, WB, Flow Cyt, ICC/IF, IHC-Pmore details
- Species reactivityReacts with: Cow, Human, Zebrafish
- Immunogen
Full length native Bovine protein, COX4 + COX4L2.
- Positive control
- Isolated mitochondria from Human heart, Bovine heart and HepG2 cells. Human skeletal muscle (frozen sections). Cultured Human embryonic lung derived fibroblasts (strain MRC5; fixed and permeabilized). Human colon tissue sections. HeLa cells.
- General notes
Product was previously marketed under the MitoSciences sub-brand.
Properties
- FormLiquid
- Storage instructionsShipped at 4°C. Store at +4°C. Do Not Freeze.
- Storage bufferPreservative: 0.02% Sodium azide
Constituent: HBS -
Concentration information loading... - PurityIgG fraction
- Purification notesab110261 was produced in vitro using hybridomas grown in serum-free medium, and then purified by biochemical fractionation. ab110261 was judged as near homogeneity by SDS PAGE.
- ClonalityMonoclonal
- Clone number10G8D12C12
- IsotypeIgG2a
- Light chain typekappa
- Research areas
Associated products
- Alternative Versions
- Compatible Secondaries
Applications
Our Abpromise guarantee covers the use of ab110261 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-Fr | 1/800. | |
| WB | Use a concentration of 1 µg/ml. Predicted molecular weight: 20 kDa. | |
| Flow Cyt | Use a concentration of 1 µg/ml. ab170191-Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody. | |
| ICC/IF | Use a concentration of 5 µg/ml. (heat-induced antigen-retrieval improves signal) | |
| IHC-P | 1/200. |
Target
- RelevanceCytochrome C Oxidase is located in the inner mitochondrial membrane and is the terminal enzyme complex of the mitochondrial electron transport chain. It collects electrons that are transferred from reduced cytochrome C and donates them to molecular oxygen, which is then reduced to water. It is composed of cytochrome A and cytochrome B, two copper atoms, and 13 different protein subunits, three of which are encoded by the mitochondrial DNA and ten others by nuclear DNA (mammals).
- Cellular localizationMitochondrial inner membrane
-
Database links
- Entrez Gene: 1327 Human
- Entrez Gene: 84701 Human
- Omim: 123864 Human
- Omim: 607976 Human
- SwissProt: P13073 Human
- SwissProt: Q96KJ9 Human
-
Alternative names
- AL024441 antibody
- COX 4 antibody
- COX IV antibody
see all
Anti-COX4 + COX4L2 antibody [10G8D12C12] images
-
![Western blot - Anti-COX4 + COX4L2 antibody [10G8D12C12 ] (ab110261)](http://a.abcam.com/ps/datasheet/images/110/ab110261/COX4-COX4L2-Primary-antibodies-ab110261-13.gif)
Western blot - Anti-COX4 + COX4L2 antibody [10G8D12C12 ] (ab110261)All lanes : Anti-COX4 + COX4L2 antibody [10G8D12C12] (ab110261) at 1 µg/ml
Lane 1 : Isolated mitochondria from Human heart at 5 µg
Lane 2 : Isolated mitochondria from Bovine heart at 1 µg
Lane 3 : Isolated mitochondria from HepG2 at 20 µg
Predicted band size : 20 kDa -
![Flow Cytometry - COX4 + COX4L2 antibody [10G8D12C12 ] (ab110261)](http://a.abcam.com/ps/datasheet/images/110/ab110261/COX4-COX4L2-Primary-antibodies-ab110261-2.JPG)
Flow Cytometry - COX4 + COX4L2 antibody [10G8D12C12 ] (ab110261)ab110261, at 1 µg/mL, staining COX4 + COX4L2 in HeLa cells by Immunohistochemistry (blue) and an isotype control antibody, at 1 µg/mL, staining of HeLa cells (red). -
![Immunocytochemistry/ Immunofluorescence - Anti-COX4 + COX4L2 antibody [10G8D12C12 ] (ab110261)](http://a.abcam.com/ps/datasheet/images/110/ab110261/COX4-COX4L2-Primary-antibodies-ab110261-14.gif)
Immunocytochemistry/ Immunofluorescence - Anti-COX4 + COX4L2 antibody [10G8D12C12 ] (ab110261)ab110261, at 10 µg/ml, staining COX4 + COX4L2 in cultured Human embryonic lung derived fibroblasts by Immunofluorescence. The cells (strain MRC5) were fixed, permeabilized and then labeled with ab110261 followed by an AlexaFluor® 488 conjugated Goat anti-mouse IgG2a isotype specific secondary antibody at 1 µg/ml. -
![Immunohistochemistry (Frozen sections) - Anti-COX4 + COX4L2 antibody [10G8D12C12 ] (ab110261)](http://a.abcam.com/ps/datasheet/images/110/ab110261/COX4-COX4L2-Primary-antibodies-ab110261-15.gif)
Immunohistochemistry (Frozen sections) - Anti-COX4 + COX4L2 antibody [10G8D12C12 ] (ab110261)ab110261, at 1/800, staining COX4 + COX4L2 in skeletal muscle tissue sections from a patient with a single large deletion of the mtDNA by Immunohistochemistry. The tissue was snap frozen and fixed in 4% PFA. Image kindly provided by Dr. J. Murphy and D. Turnbull, Mitochondrial Research Group, Newcastle University. -
![Immunohistochemistry (Frozen sections) - Anti-COX4 + COX4L2 antibody [10G8D12C12 ] (ab110261)](http://a.abcam.com/ps/datasheet/images/110/ab110261/COX4-COX4L2-Primary-antibodies-ab110261-18.gif)
Immunohistochemistry (Frozen sections) - Anti-COX4 + COX4L2 antibody [10G8D12C12 ] (ab110261)ab110261, at 1/200, showing COX4 + COX4L2 negative crypts in colon tissue sections from a normal ageing patient by Immunohistochemistry. The tissue was snap frozen and fixed in 4% PFA. Image kindly provided by Dr. J. Murphy and D. Turnbull, Mitochondrial Research Group, Newcastle University.
References for Anti-COX4 + COX4L2 antibody [10G8D12C12] (ab110261)
This product has been referenced in:
- Yang T et al. A small molecule TrkB/TrkC neurotrophin receptor co-activator with distinctive effects on neuronal survival and process outgrowth. Neuropharmacology 110:343-61 (2016). Read more (PubMed: 27334657) »
- Qiu F et al. Arginine starvation impairs mitochondrial respiratory function in ASS1-deficient breast cancer cells. Sci Signal 7:ra31 (2014). Human . Read more (PubMed: 24692592) »
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"