Overview

  • Product name
    Anti-COX5A antibody [EPR14208(B)]
    See all COX5A primary antibodies
  • Description
    Rabbit monoclonal [EPR14208(B)] to COX5A
  • Host species
    Rabbit
  • Tested applications
    Suitable for: Flow Cyt, IHC-P, WB, ICC/IF, IPmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human COX5A aa 100 to the C-terminus. The exact sequence is proprietary.
    Database link: P20674

  • Positive control
    • HeLa, HepG2, A431 cells, Human fetal heart lysates; HeLa cells; Human kidney tissue.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 0.05% BSA, 40% Glycerol
  • Purity
    Tissue culture supernatant
  • Clonality
    Monoclonal
  • Clone number
    EPR14208(B)
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab180129 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt 1/200.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 

IHC-P 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/2000 - 1/10000. Detects a band of approximately 13 kDa (predicted molecular weight: 17 kDa).
ICC/IF 1/100.
IP 1/50.

Target

Images

  • All lanes : Anti-COX5A antibody [EPR14208(B)] (ab180129) at 1/5000 dilution

    Lane 1 : HeLa cell lysate
    Lane 2 : HepG2 cell lysate
    Lane 3 : A431 cell lysate
    Lane 4 : Human fetal heart lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 17 kDa



    The observed MW is lower than the predicted 17kDa, which may be due to the removing of 4KDa transit peptide.

  • Immunoprecipitation analysis of HeLa cells labeling COX5A using ab180129 at 1/50 dilution. A Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/2000 was used as secondary antibody.

  • Immunohistochemical analysis of paraffin-embedded human kidney tissue labeling COX5A using ab180129 at 1/500 dilution. A Goat anti rabbit IgG (HRP) prediluted was used as secondary. Counterstain: Hematoxylin.

  • Immunofluorescence analysis of HeLa cells labeling COX5A using ab180129 at 1/100 dilution. A Goat anti rabbit IgG (Dylight 555) at 1/250 dilution was used as secondary antibody. Cells were fixed with 4% paraformaldehyde. Couterstain: DAPI.

  • Flow cytometry analysis of HeLa cells labeling COX5A using ab180129 at 1/200 dilution. A Goat anti rabbit IgG (FITC) at 1/75 dilution was used as secondary antibody. Cells were fixed with 2% paraformaldehyde. Isotype control: Rabbit IgG.

References

This product has been referenced in:
  • Das S  et al. miR-181c Regulates the Mitochondrial Genome, Bioenergetics, and Propensity for Heart Failure In Vivo. PLoS One 9:e96820 (2014). WB ; Rat . Read more (PubMed: 24810628) »

See 1 Publication for this product

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab180129.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up