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Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human COX5A aa 100 to the C-terminus. The exact sequence is proprietary.
Database link: P20674
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents
This product is a recombinant rabbit monoclonal antibody.
Our Abpromise guarantee covers the use of ab180129 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
|IHC-P||1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|WB||1/2000 - 1/10000. Detects a band of approximately 13 kDa (predicted molecular weight: 17 kDa).|
The observed MW is lower than the predicted 17kDa, which may be due to the removing of 4KDa transit peptide.
Immunoprecipitation analysis of HeLa cells labeling COX5A using ab180129 at 1/50 dilution. A Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/2000 was used as secondary antibody.
Immunohistochemical analysis of paraffin-embedded human kidney tissue labeling COX5A using ab180129 at 1/500 dilution. A Goat anti rabbit IgG (HRP) prediluted was used as secondary. Counterstain: Hematoxylin.
Immunofluorescence analysis of HeLa cells labeling COX5A using ab180129 at 1/100 dilution. A Goat anti rabbit IgG (Dylight 555) at 1/250 dilution was used as secondary antibody. Cells were fixed with 4% paraformaldehyde. Couterstain: DAPI.
Flow cytometry analysis of HeLa cells labeling COX5A using ab180129 at 1/200 dilution. A Goat anti rabbit IgG (FITC) at 1/75 dilution was used as secondary antibody. Cells were fixed with 2% paraformaldehyde. Isotype control: Rabbit IgG.