Overview

  • Product nameAnti-COX5B antibody
    See all COX5B primary antibodies
  • Description
    Mouse polyclonal to COX5B
  • Tested applicationsSuitable for: WB, ICC/IF, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Full length Human protein (NP_001853.2)

  • Positive control
    • Human pancreas tissue lysate, HepG2 cell lysate, transfected 293T cell lysate, HeLa cells. Human hippocampus FFPE tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab88440 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 14 kDa.
ICC/IF Use a concentration of 10 µg/ml.
IHC-P Use a concentration of 1 µg/ml.

Target

  • FunctionThis protein is one of the nuclear-coded polypeptide chains of cytochrome c oxidase, the terminal oxidase in mitochondrial electron transport.
  • Sequence similaritiesBelongs to the cytochrome c oxidase subunit 5B family.
  • Cellular localizationMitochondrion inner membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • COX 5B antibody
    • COX5B antibody
    • COX5B_HUMAN antibody
    • COXVB antibody
    • Cytochrome c oxidase polypeptide Vb antibody
    • Cytochrome c oxidase polypeptide VB mitochondrial antibody
    • Cytochrome c oxidase subunit 5B antibody
    • Cytochrome c oxidase subunit 5B mitochondrial antibody
    • Cytochrome c oxidase subunit Vb antibody
    • mitochondrial antibody
    see all

Anti-COX5B antibody images

  • Anti-COX5B antibody (ab88440) at 1 µg/ml + human pancreas tissue lysate at 50 µg
    Developed using the ECL technique

    Predicted band size : 14 kDa
  • Anti-COX5B antibody (ab88440) at 1 µg/ml + HepG2 cell lysate at 50 µg
    Developed using the ECL technique

    Predicted band size : 14 kDa
  • All lanes : Anti-COX5B antibody (ab88440) at 1 µg/ml

    Lane 1 : cell lysate from COX5B transfected 293T cells
    Lane 2 : cell lysate from non transfected 293T cells

    Lysates/proteins at 25 µg per lane.

    Developed using the ECL technique

    Predicted band size : 14 kDa
    Observed band size : 14 kDa
  • ab88440 at 10 µg/ml staining COX5B in paraformaldehyde fixed, permeabilised HeLa cells by immunofluorescence.
  • IHC image of COX5B staining in Human hippocampus formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab88440, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

References for Anti-COX5B antibody (ab88440)

ab88440 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"