Overview

  • Product name
    Anti-COX6A1 antibody [14A3AD2BH4]
    See all COX6A1 primary antibodies
  • Description
    Mouse monoclonal [14A3AD2BH4] to COX6A1
  • Specificity
    ab110265 recognises the Liver isoform in human, rat, mouse.
  • Tested applications
    Suitable for: Flow Cyt, WBmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
    Predicted to work with: Rabbit, Cow, Dog
  • Immunogen

    Synthetic peptide:

    SSGAHGEEGSARMWKALTLF(C)

    , corresponding to amino acids 25-44 of Cow COX6A1

  • Positive control
    • HepG2, Human, Mouse and Rat heart lysate.
  • General notes

    This antibody clone is manufactured by Abcam.

    Product was previously marketed under the MitoSciences sub-brand.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C. Do Not Freeze.
  • Storage buffer
    Preservative: 0.02% Sodium azide
    Constituent: HBS
  • Concentration information loading...
  • Purification notes
    ab110265 was produced in vitro using hybridomas grown in serum-free medium, and then purified by biochemical fractionation.
  • Clonality
    Monoclonal
  • Clone number
    14A3AD2BH4
  • Isotype
    IgG1
  • Light chain type
    kappa
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab110265 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use 1µg for 106 cells. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
WB Use a concentration of 5 µg/ml. Predicted molecular weight: 12 kDa.

Target

  • Function
    This protein is one of the nuclear-coded polypeptide chains of cytochrome c oxidase, the terminal oxidase in mitochondrial electron transport.
  • Sequence similarities
    Belongs to the cytochrome c oxidase subunit 6A family.
  • Cellular localization
    Mitochondrion inner membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • COX VIa L antibody
    • COX VIa-L antibody
    • COX6A antibody
    • COX6A1 antibody
    • COX6AL antibody
    • CX6A1_HUMAN antibody
    • Cytochrome c oxidase polypeptide VIa liver antibody
    • Cytochrome c oxidase polypeptide VIa-liver antibody
    • Cytochrome c oxidase subunit 6A1 antibody
    • Cytochrome c oxidase subunit 6A1 mitochondrial antibody
    • Cytochrome C oxidase subunit VIa homolog antibody
    • Cytochrome c oxidase subunit VIA liver antibody
    • Cytochrome c oxidase subunit VIa polypeptide 1 antibody
    • Cytochrome c oxidase subunit VIA-liver antibody
    • MGC104500 antibody
    • mitochondrial antibody
    see all

Images

  • Overlay histogram showing HepG2 cells stained with ab110265 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab110265, 1μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H&L) (ab150113) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in HepG2 cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.
  • All lanes : Anti-COX6A1 antibody [14A3AD2BH4] (ab110265) at 5 µg/ml

    Lane 1 : Human Heart Lysate
    Lane 2 : Rat Heart Lysate
    Lane 3 : Mouse Heart Lysate
    Lane 4 : HepG2 cell lysate


    Predicted band size : 12 kDa
    Extra bands in the mouse sample (lane 3) are due to the reaction of the IgG-specific goat anti-mouse secondary antibody with residual mouse blood in the heart tissue, as it is very difficult to entirely remove the blood from these small organs.

References

This product has been referenced in:
  • Sohal RS  et al. Age-related decrease in expression of mitochondrial DNA encoded subunits of cytochrome c oxidase in Drosophila melanogaster. Mech Ageing Dev 129:558-61 (2008). Read more (PubMed: 18538373) »

See 1 Publication for this product

Customer reviews and Q&As

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Mouse Cell lysate - other (brain mito)
Gel Running Conditions
Non-reduced Denaturing (NuPAGE 4-12%)
Loading amount
20 µg
Specification
brain mito
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Username

Abcam user community

Verified customer

Submitted Jul 14 2017

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up