Overview

  • Product name
    Anti-COX6A1 antibody [14A3AD2BH4]
    See all COX6A1 primary antibodies
  • Description
    Mouse monoclonal [14A3AD2BH4] to COX6A1
  • Specificity
    ab110265 recognises the Liver isoform in human, rat, mouse.
  • Tested applications
    Suitable for: Flow Cyt, WBmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
    Predicted to work with: Rabbit, Cow, Dog
  • Immunogen

    Synthetic peptide:

    SSGAHGEEGSARMWKALTLF(C)

    , corresponding to amino acids 25-44 of Cow COX6A1

  • Positive control
    • HepG2, Human, Mouse and Rat heart lysate.
  • General notes

    This antibody clone is manufactured by Abcam.

    Product was previously marketed under the MitoSciences sub-brand.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C. Do Not Freeze.
  • Storage buffer
    Preservative: 0.02% Sodium azide
    Constituent: HBS
  • Concentration information loading...
  • Purification notes
    ab110265 was produced in vitro using hybridomas grown in serum-free medium, and then purified by biochemical fractionation.
  • Clonality
    Monoclonal
  • Clone number
    14A3AD2BH4
  • Isotype
    IgG1
  • Light chain type
    kappa
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab110265 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use 1µg for 106 cells. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
WB Use a concentration of 5 µg/ml. Predicted molecular weight: 12 kDa.

Target

  • Function
    This protein is one of the nuclear-coded polypeptide chains of cytochrome c oxidase, the terminal oxidase in mitochondrial electron transport.
  • Sequence similarities
    Belongs to the cytochrome c oxidase subunit 6A family.
  • Cellular localization
    Mitochondrion inner membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • COX VIa L antibody
    • COX VIa-L antibody
    • COX6A antibody
    • COX6A1 antibody
    • COX6AL antibody
    • CX6A1_HUMAN antibody
    • Cytochrome c oxidase polypeptide VIa liver antibody
    • Cytochrome c oxidase polypeptide VIa-liver antibody
    • Cytochrome c oxidase subunit 6A1 antibody
    • Cytochrome c oxidase subunit 6A1 mitochondrial antibody
    • Cytochrome C oxidase subunit VIa homolog antibody
    • Cytochrome c oxidase subunit VIA liver antibody
    • Cytochrome c oxidase subunit VIa polypeptide 1 antibody
    • Cytochrome c oxidase subunit VIA-liver antibody
    • MGC104500 antibody
    • mitochondrial antibody
    see all

Anti-COX6A1 antibody [14A3AD2BH4] images

  • Overlay histogram showing HepG2 cells stained with ab110265 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab110265, 1μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H&L) (ab150113) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in HepG2 cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.
  • All lanes : Anti-COX6A1 antibody [14A3AD2BH4] (ab110265) at 5 µg/ml

    Lane 1 : Human Heart Lysate
    Lane 2 : Rat Heart Lysate
    Lane 3 : Mouse Heart Lysate
    Lane 4 : HepG2 cell lysate


    Predicted band size : 12 kDa
    Extra bands in the mouse sample (lane 3) are due to the reaction of the IgG-specific goat anti-mouse secondary antibody with residual mouse blood in the heart tissue, as it is very difficult to entirely remove the blood from these small organs.

References for Anti-COX6A1 antibody [14A3AD2BH4] (ab110265)

This product has been referenced in:
  • Sohal RS  et al. Age-related decrease in expression of mitochondrial DNA encoded subunits of cytochrome c oxidase in Drosophila melanogaster. Mech Ageing Dev 129:558-61 (2008). Read more (PubMed: 18538373) »

See 1 Publication for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Mouse Cell lysate - other (brain mito)
Gel Running Conditions
Non-reduced Denaturing (NuPAGE 4-12%)
Loading amount
20 µg
Specification
brain mito
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
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Submitted Jul 14 2017

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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