Anti-Coxiella burnetii Phase 2 IgG ELISA Kit (Q-Fever) (ab178639)

Overview

  • Product name
    Anti-Coxiella burnetii Phase 2 IgG ELISA Kit (Q-Fever)
    See all Coxiella burnetii IgG kits
  • Detection method
    Colorimetric
  • Precision
    Intra-assay
    Sample n Mean SD CV%
    Greyzone 15 3.4%
    Pos serum 16 2.6%
    Inter-assay
    Sample n Mean SD CV%
    Greyzone 5 10.1NTU/ml 10.8%
    Pos serum 16 19.5NTU/ml 1.7%
  • Sample type
    Serum
  • Assay type
    Indirect
  • Sensitivity
    > 90 %
  • Assay duration
    Multiple steps standard assay
  • Species reactivity
    Reacts with: Human
  • Product overview

    Abcam’s anti-Coxiella burnetii (Q-Fever) Phase 2 IgG Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate qualitative measurement of IgG class antibodies against Coxiella burnetii in Human serum. 


    A 96-well plate has been precoated with Coxiella burnetii antigens to bind cognate antibodies. Controls or test samples are added to the wells and incubated. Following washing, a horseradish peroxidase (HRP) labelled anti-Human IgG conjugate is added to the wells, which binds to the immobilized Coxiella burnetii antigens. TMB is then catalyzed by the HRP to produce a blue color product that changes to yellow after adding an acidic stop solution. The intensity of yellow coloration is directly proportional to the amount of Coxiella burnetii IgG sample captured in plate.

  • Tested applications
    Suitable for: Indirect ELISAmore details
  • Platform
    Microplate

Properties

  • Storage instructions
    Store at +4°C. Please refer to protocols.
  • Components Identifier 1 x 96 tests
    20X Washing Solution White cap 1 x 50ml
    Cover Foil 1 unit
    Coxiella burnetii Anti-IgG HRP Conjugate 1 x 20ml
    Coxiella burnetii IgG Cut-off Control 1 x 3ml
    Coxiella burnetii IgG Negative Control 1 x 2ml
    Coxiella burnetii IgG Positive Control 1 x 2ml
    Coxiella burnetii Phase 2 (IgG) Coated Microplate (12 x 8 wells) 1 unit
    IgG Sample Diluent 1 x 100ml
    Stop Solution 1 x 15ml
    Strip holder 1 unit
    TMB Substrate Solution Yellow cap 1 x 15ml
  • Research areas
  • Relevance
    Coxiella burnetii is an obligate intracellular bacterial pathogen, and is the causative agent of Q fever. The genus Coxiella is morphologically similar to Rickettsia, but with a variety of genetic and physiological differences. C. burnetii is a small Gram-negative bacterium that is highly resistant to environmental stresses such as high temperature, osmotic pressure, and ultraviolet light. These characteristics are attributed to a small cell variant (SCV) form of the organism that is part of a biphasic developmental cycle, including a more metabolically and replicatively active large cell variant (LCV) form. It can survive standard disinfectants, and is resistant to many other environmental changes like those presented in the phagolysosome.

Applications

Our Abpromise guarantee covers the use of ab178639 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Indirect ELISA Use at an assay dependent concentration.

Protocols

References

ab178639 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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