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Abcam’s anti-Coxiella burnetii (Q-Fever) Phase 2 IgG Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate qualitative measurement of IgG class antibodies against Coxiella burnetii in Human serum.
A 96-well plate has been precoated with Coxiella burnetii antigens to bind cognate antibodies. Controls or test samples are added to the wells and incubated. Following washing, a horseradish peroxidase (HRP) labelled anti-Human IgG conjugate is added to the wells, which binds to the immobilized Coxiella burnetii antigens. TMB is then catalyzed by the HRP to produce a blue color product that changes to yellow after adding an acidic stop solution. The intensity of yellow coloration is directly proportional to the amount of Coxiella burnetii IgG sample captured in plate.
|Components||Identifier||1 x 96 tests|
|20X Washing Solution||White cap||1 x 50ml|
|Cover Foil||1 unit|
|Coxiella burnetii Anti-IgG HRP Conjugate||1 x 20ml|
|Coxiella burnetii IgG Cut-off Control||1 x 3ml|
|Coxiella burnetii IgG Negative Control||1 x 2ml|
|Coxiella burnetii IgG Positive Control||1 x 2ml|
|Coxiella burnetii Phase 2 (IgG) Coated Microplate (12 x 8 wells)||1 unit|
|IgG Sample Diluent||1 x 100ml|
|Stop Solution||1 x 15ml|
|Strip holder||1 unit|
|TMB Substrate Solution||Yellow cap||1 x 15ml|
Our Abpromise guarantee covers the use of ab178639 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Indirect ELISA||Use at an assay dependent concentration.|
ab178639 has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"