Overview

  • Product name
    Anti-COXIV Isoform 2 antibody
  • Description
    Rabbit polyclonal to COXIV Isoform 2
  • Specificity
    ab70112 detects endogenous levels of total COXIV Isoform 2 protein.
  • Tested applications
    Suitable for: ELISA, WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide corresponding to Human COXIV Isoform 2 aa 31-80 (internal sequence).
    Database link: Q96KJ9

  • Positive control
    • COS7 cells and K562 cell extracts treated with insulin (0.01U/ml, 15mins).

Properties

Applications

Our Abpromise guarantee covers the use of ab70112 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/10000.
WB 1/500 - 1/1000. Detects a band of approximately 20 kDa (predicted molecular weight: 20 kDa).
ICC/IF 1/500 - 1/1000.

Target

  • Function
    This protein is one of the nuclear-coded polypeptide chains of cytochrome c oxidase, the terminal oxidase in mitochondrial electron transport.
  • Tissue specificity
    Highly expressed in lung.
  • Involvement in disease
    Defects in COX4I2 are the cause of exocrine pancreatic insufficiency dyserythropoietic anemia and calvarial hyperostosis (EPIDACH) [MIM:612714]. Patients present with pancreatic insufficiency, intestinal malabsorption, failure to thrive, and anemia soon after birth.
  • Sequence similarities
    Belongs to the cytochrome c oxidase IV family.
  • Cellular localization
    Mitochondrion inner membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • COX IV-2 antibody
    • COX4 2 antibody
    • COX4 antibody
    • COX42_HUMAN antibody
    • COX4B antibody
    • Cox4i2 antibody
    • COX4L2 antibody
    • COXIV 2 antibody
    • Cytochrome c oxidase subunit 4 isoform 2 antibody
    • Cytochrome c oxidase subunit 4 isoform 2 mitochondrial [Precursor] antibody
    • cytochrome c oxidase subunit IV isoform 2 (lung) antibody
    • Cytochrome c oxidase subunit IV isoform 2 antibody
    • dJ857M17.2 antibody
    • mitochondrial antibody
    • RP23 106A3.3 antibody
    see all

Images

  • ab70112 at 1/500 dilution staining COXIV Isoform 2 in COS7 cells by Immunofluorescence, in the absence or presence of the immunising peptide.
  • All lanes : Anti-COXIV Isoform 2 antibody (ab70112) at 1/500 dilution

    Lane 1 : K562 cell extracts treated with insulin (0.01U/ml, 15mins)
    Lane 2 : K562 cell extracts treated with insulin (0.01U/ml, 15mins) with immunising peptide at 10 µg

    Lysates/proteins at 30 µg per lane.


    Predicted band size : 20 kDa
    Observed band size : 20 kDa

References

This product has been referenced in:
  • Lou PH  et al. Loss of Intralipid®- but not sevoflurane-mediated cardioprotection in early type-2 diabetic hearts of fructose-fed rats: importance of ROS signaling. PLoS One 9:e104971 (2014). WB ; Rat . Read more (PubMed: 25127027) »
  • Head B  et al. Inducible proteolytic inactivation of OPA1 mediated by the OMA1 protease in mammalian cells. J Cell Biol 187:959-66 (2009). Read more (PubMed: 20038677) »

See all 3 Publications for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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