Anti-Coxsackie Adenovirus Receptor antibody (ab82726)

Overview

  • Product nameAnti-Coxsackie Adenovirus Receptor antibody
    See all Coxsackie Adenovirus Receptor primary antibodies
  • Description
    Rabbit polyclonal to Coxsackie Adenovirus Receptor
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Cow, Cat, Dog, Pig
  • Immunogen

    Synthetic peptide: ILYSGDKIYD DYYPDLKGRV HFTSNDLKSG DASINVTNLQ LSDIGTYQCK , corresponding to a region within N terminal amino acids 72 - 121 of Human Coxsackie Adenovirus Receptor (NP_001329)

  • Positive control
    • Fetal brain lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab82726 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 41 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

Target

  • FunctionComponent of the epithelial apical junction complex that is essential for the tight junction integrity. Proposed to function as a homophilic cell adhesion molecule. Recruits MPDZ to intercellular contact sites. Probably involved in transepithelial migration of polymorphonuclear leukocytes (PMN) through adhesive interactions with AMICA1/JAML located in the plasma membrane of PMN.
  • Tissue specificityExpressed in pancreas, brain, heart, small intestine, testis, prostate and at a lower level in liver and lung. Isoform 5 is ubiquitously expressed. Isoform 3 is expressed in heart, lung and pancreas. In skeletal muscle, isoform 1 is found at the neuromuscular junction and isoform 2 is found in blood vessels. In cardiac muscle, isoform 1 and isoform 2 are found at intercalated disks. In heart expressed in subendothelial layers of the vessel wall but not in the luminal endothelial surface. Expression is elevated in hearts with dilated cardiomyopathy.
  • Sequence similaritiesContains 2 Ig-like C2-type (immunoglobulin-like) domains.
  • DomainThe Ig-like C2-type 1 domain probably mediates homodimerization and interaction with JAML.
    The PDZ-binding motif mediates interaction with MPDZ and BAIAP1.
  • Post-translational
    modifications
    N-glycosylated.
    Palmitoylated on Cys-259 and/or Cys-260; required for proper localization to the plasma membrane.
  • Cellular localizationSecreted and Cell membrane. Cell junction > tight junction. Cell junction > adherens junction. Basolateral cell membrane. In epithelial cells localizes to the apical junction complex composced of tight and adherens junctions. In airway epithelial cells localized to basolateral membrane but not to apical surface.
  • Information by UniProt
  • Database links
  • Alternative names
    • 46 kD coxsackievirus and adenovirus receptor CAR protein antibody
    • 46 kD coxsackievirus and adenovirus receptor protein antibody
    • CAR antibody
    • CAR4/6 antibody
    • Coxsackie virus and adenovirus receptor antibody
    • Coxsackie virus B receptor antibody
    • Coxsackievirus and adenovirus receptor antibody
    • Coxsackievirus B adenovirus receptor antibody
    • Coxsackievirus B-adenovirus receptor antibody
    • CVB3 binding protein antibody
    • CVB3-binding protein antibody
    • Cxadr antibody
    • CXAR_HUMAN antibody
    • hCAR antibody
    • HCVADR antibody
    see all

Anti-Coxsackie Adenovirus Receptor antibody images

  • Anti-Coxsackie Adenovirus Receptor antibody (ab82726) at 1 µg/ml + Fetal brain lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 41 kDa
    Observed band size : 41 kDa

References for Anti-Coxsackie Adenovirus Receptor antibody (ab82726)

ab82726 has not yet been referenced specifically in any publications.

Product Wall

Application Western blot
Loading amount 30 µg
Gel Running Conditions Reduced Denaturing (4-12% gradient)
Sample Mouse Tissue lysate - whole (Testis)
Specification Testis
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Username

Dr. David Simmons

Verified customer

Submitted Aug 18 2014

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"