Overview

  • Product nameAnti-CP110 antibody
    See all CP110 primary antibodies
  • Description
    Rabbit polyclonal to CP110
  • Tested applicationsSuitable for: WB, IPmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Rabbit, Horse, Guinea pig, Cow, Dog, Pig, Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Elephant
  • Immunogen

    Synthetic peptide corresponding to a region within amino acids 962-1012 of Human CP110 (CAH18111.1).

  • Positive control
    • HeLa and 293T whole cell lysates

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab99338 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000. Predicted molecular weight: 113 kDa.
IP Use at 2-5 µg/mg of lysate.

Target

  • FunctionNecessary for centrosome duplication at different stages of procentriole formation. Collaborates with CEP97, being involved in the suppression of a cilia assembly program. Required for correct spindle formation and has a role in regulating cytokinesis and genome stability via cooperation with CALM1 and CETN2.
  • Tissue specificityHighly expressed in testis. Detected at intermediate levels in spleen, thymus, prostate, small intestine, colon and peripheral blood leukocytes.
  • Post-translational
    modifications
    Phosphorylated by CDKs.
    Ubiquitinated by the SCF(Cyclin F) during G2 phase, leading to its degradation by the proteasome and preventing centrosome reduplication.
  • Cellular localizationCytoplasm > cytoskeleton > centrosome > centriole. Recruited early and then associates with the growing distal tips.
  • Information by UniProt
  • Database links
  • Alternative names
    • 110 kDa centrosomal protein antibody
    • CCP110 antibody
    • Centriolar coiled coil protein 110kDa antibody
    • Centrosomal protein 110 kDa antibody
    • Centrosomal protein CP110 antibody
    • Centrosomal protein of 110 kDa antibody
    • Cep110 antibody
    • CP110 antibody
    • CP110_HUMAN antibody
    • DKFZp781G1416 antibody
    • FAN antibody
    • KIAA0419 antibody
    see all

Anti-CP110 antibody images

  • All lanes : Anti-CP110 antibody (ab99338) at 0.04 µg/ml

    Lane 1 : Hela whole cell lysate at 50 µg
    Lane 2 : Hela whole cell lysate at 15 µg
    Lane 3 : Hela whole cell lysate at 5 µg
    Lane 4 : 293T whole cell lysate at 50 µg

    Developed using the ECL technique

    Predicted band size : 113 kDa


    Exposure time : 30 seconds
  • Detection of Human CP110 by Immunoprecipitation (IP) from HeLa whole cell lysate (1 mg for IP, 20% of IP loaded).
    1. IP with a CP110 antibody which recognizes an upstream epitope
    2. IP with ab99338 at 3 µg/mg lysate
    3. Control IgG IP
    Subsequent WB detection was performed using ab99338 at 1 µg/ml.

References for Anti-CP110 antibody (ab99338)

ab99338 has not yet been referenced specifically in any publications.

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