Anti-CP110 antibody (ab99338)
Key features and details
- Rabbit polyclonal to CP110
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-CP110 antibody
See all CP110 primary antibodies -
Description
Rabbit polyclonal to CP110 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IPmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Rabbit, Horse, Guinea pig, Cow, Dog, Pig, Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Elephant -
Immunogen
Synthetic peptide corresponding to a region within amino acids 962-1012 of Human CP110 (CAH18111.1).
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Positive control
- HeLa and 293T whole cell lysates
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab99338 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/2000 - 1/10000. Predicted molecular weight: 113 kDa.
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IP |
Use at 2-5 µg/mg of lysate.
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Notes |
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WB
1/2000 - 1/10000. Predicted molecular weight: 113 kDa. |
IP
Use at 2-5 µg/mg of lysate. |
Target
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Function
Necessary for centrosome duplication at different stages of procentriole formation. Collaborates with CEP97, being involved in the suppression of a cilia assembly program. Required for correct spindle formation and has a role in regulating cytokinesis and genome stability via cooperation with CALM1 and CETN2. -
Tissue specificity
Highly expressed in testis. Detected at intermediate levels in spleen, thymus, prostate, small intestine, colon and peripheral blood leukocytes. -
Post-translational
modificationsPhosphorylated by CDKs.
Ubiquitinated by the SCF(Cyclin F) during G2 phase, leading to its degradation by the proteasome and preventing centrosome reduplication. -
Cellular localization
Cytoplasm > cytoskeleton > centrosome > centriole. Recruited early and then associates with the growing distal tips. - Information by UniProt
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Database links
- Entrez Gene: 9738 Human
- Omim: 609544 Human
- SwissProt: O43303 Human
- Unigene: 279912 Human
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Alternative names
- 110 kDa centrosomal protein antibody
- CCP110 antibody
- Centriolar coiled coil protein 110kDa antibody
see all
Images
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All lanes : Anti-CP110 antibody (ab99338) at 0.04 µg/ml
Lane 1 : Hela whole cell lysate at 50 µg
Lane 2 : Hela whole cell lysate at 15 µg
Lane 3 : Hela whole cell lysate at 5 µg
Lane 4 : 293T whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 113 kDa
Exposure time: 30 seconds -
Detection of Human CP110 by Immunoprecipitation (IP) from HeLa whole cell lysate (1 mg for IP, 20% of IP loaded).
1. IP with a CP110 antibody which recognizes an upstream epitope
2. IP with ab99338 at 3 µg/mg lysate
3. Control IgG IP
Subsequent WB detection was performed using ab99338 at 1 µg/ml.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (4)
ab99338 has been referenced in 4 publications.
- Chao YY et al. ATM- and ATR-induced primary ciliogenesis promotes cisplatin resistance in pancreatic ductal adenocarcinoma. J Cell Physiol 237:4487-4503 (2022). PubMed: 36251015
- Chen TY et al. Genotoxic stress-activated DNA-PK-p53 cascade and autophagy cooperatively induce ciliogenesis to maintain the DNA damage response. Cell Death Differ 28:1865-1879 (2021). PubMed: 33462409
- Chen TY et al. Septin 7 is a centrosomal protein that ensures S phase entry and microtubule nucleation by maintaining the abundance of p150glued. J Cell Physiol 236:2706-2724 (2021). PubMed: 32869310
- Lai Y et al. Inflammation-mediated upregulation of centrosomal protein 110, a negative modulator of ciliogenesis, in patients with chronic rhinosinusitis. J Allergy Clin Immunol 128:1207-1215.e1 (2011). PubMed: 21982113