Overview

  • Product nameAnti-CPEB1 antibody
    See all CPEB1 primary antibodies
  • Description
    Rabbit polyclonal to CPEB1
  • SpecificityDetects recombinant mouse CPEB1.
  • Tested applicationsSuitable for: IP, WBmore details
  • Species reactivity
    Reacts with: Mouse
    Predicted to work with: Rat, Human, Xenopus laevis
  • Immunogen

    Synthetic peptide corresponding to Human CPEB1 aa 545-562.
    Sequence:

    HSMEGLRHHSPLMRNQKN


    (Peptide available as ab4988)

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab3465 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP Use at an assay dependent concentration.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 65 kDa (predicted molecular weight: 65 kDa).Can be blocked with Human CPEB1 peptide (ab4988).

Target

  • FunctionSequence-specific RNA-binding protein that regulates mRNA cytoplasmic polyadenylation and translation initiation during oocyte maturation, early development and at postsynapse sites of neurons. Binds to the cytoplasmic polyadenylation element (CPE), an uridine-rich sequence element (consensus sequence 5'-UUUUUAU-3') within the mRNA 3'-UTR. In absence of phosphorylation and in association with TACC3 is also involved as a repressor of translation of CPE-containing mRNA; a repression that is relieved by phosphorylation or degradation (By similarity). Involved in the transport of CPE-containing mRNA to dendrites; those mRNAs may be transported to dendrites in a translationally dormant form and translationally activated at synapses (By similarity). Its interaction with APLP1 promotes local CPE-containing mRNA polyadenylation and translation activation (By similarity). Induces the assembly of stress granules in the absence of stress.
  • Tissue specificityIsoform 1 is expressed in immature oocytes, ovary, brain and heart. Isoform 2 is expressed in brain and heart. Isoform 3 and isoform 4 are expressed in brain. Expressed in breast tumors and several tumor cell lines.
  • Sequence similaritiesBelongs to the RRM CPEB family.
    Contains 2 RRM (RNA recognition motif) domains.
  • DomainThe 2 RRM domains and the C-terminal region mediate interaction with CPE-containing RNA.
  • Post-translational
    modifications
    Phosphorylated on serine/threonine residues by AURKA/STK6 within positions 166 and 197. Phosphorylation and dephosphorylation on Thr-172 regulates cytoplasmic polyadenylation and translation of CPE-containing mRNAs. Phosphorylation on Thr-172 by AURKA/STK6 and CAMK2A activates CPEB1. Phosphorylation on Thr-172 may be promoted by APLP1. Phosphorylation increases binding to RNA.
  • Cellular localizationCytoplasm > P-body. Cytoplasmic granule. Cell junction > synapse. Membrane. Cell junction > synapse > postsynaptic cell membrane > postsynaptic density. Cell projection > dendrite. Also found in stress granules. Recruited to stress granules (SGs) upon arsenite treatment. In dendrites (By similarity). Localizes in synaptosomes at dendritic synapses of neurons (By similarity). Strongly enriched in postsynaptic density (PSD) fractions (By similarity). Transported into dendrites in a microtubule-dependent fashion and colocalizes in mRNA-containing particles with TACC3, dynein and kinesin (By similarity). Membrane-associated (By similarity). Colocalizes at excitatory synapses with members of the polyadenylation and translation complex factors (CPSF, APLP1, TACC3, AURKA/STK6, SYP, etc.) including CPE-containing RNAs.
  • Information by UniProt
  • Database links
  • Alternative names
    • CEBP antibody
    • CPE binding protein 1 antibody
    • CPE BP1 antibody
    • CPE-binding protein 1 antibody
    • CPE-BP1 antibody
    • CPEB 1 antibody
    • CPEB antibody
    • CPEB-1 antibody
    • CPEB1 antibody
    • CPEB1 protein antibody
    • CPEB1_HUMAN antibody
    • Cytoplasmic polyadenylation binding protein 1 antibody
    • Cytoplasmic polyadenylation element binding protein antibody
    • Cytoplasmic polyadenylation element binding protein 1 antibody
    • Cytoplasmic polyadenylation element-binding protein 1 antibody
    • FLJ13203 antibody
    • h CEBP antibody
    • h-CEBP antibody
    • hCPEB 1 antibody
    • hCPEB-1 antibody
    • MGC34136 antibody
    • MGC60106 antibody
    • mKIAA0940 antibody
    • MKIAA0940 protein antibody
    see all

Anti-CPEB1 antibody images

  • All lanes : Anti-CPEB1 antibody (ab3465) at 1/500 dilution

    Lane 1 : HeLa lysate
    Lane 2 : Human brain tissue lysate
    Lane 3 : Mouse brain tissue lysate

    Lysates/proteins at 25 µg per lane.

    Secondary
    HRP-conjugated anti-rabbit
    developed using the ECL technique

    Predicted band size : 65 kDa
    Observed band size : 62 kDa (why is the actual band size different from the predicted?)


    Exposure time : 1 minute


  • Predicted band size : 65 kDa

    Western blot detection of 1.0 ng of recombinant mouse CPEB1 using ab3465.

  • Immunohistochemistry analysis of CPEB showing staining in the cytoplasm of paraffin-embedded human hearat tissue (right) compared to a negative control without primary antibody (left). To expose target proteins, antigen retrieval was performed using 10 mM sodium citrate (pH 6.0), microwaved for 8-15 min. Following antigen retrieval, tissues were blocked in 3% H2O2-methanol for 15 min at room temperature, washed with ddH2O and PBS, and then probed with ab3465 diluted in 3% BSA-PBS at a dilution of 1/50 overnight at 4°C in a humidified chamber. Tissues were washed extensively in PBST and detection was performed using an HRP-conjugated secondary antibody followed by colorimetric detection using a DAB kit. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting.

  • Immunohistochemistry analysis of CPEB showing staining in the cytoplasm of paraffin-embedded mouse brain tissue (right) compared to a negative control without primary antibody (left). To expose target proteins, antigen retrieval was performed using 10 mM sodium citrate (pH 6.0), microwaved for 8-15 min. Following antigen retrieval, tissues were blocked in 3% H2O2-methanol for 15 min at room temperature, washed with ddH2O and PBS, and then probed with ab3465 diluted in 3% BSA-PBS at a dilution of 1/200 overnight at 4°C in a humidified chamber. Tissues were washed extensively in PBST and detection was performed using an HRP-conjugated secondary antibody followed by colorimetric detection using a DAB kit. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting.

  • Immunohistochemistry analysis of CPEB showing staining in the cytoplasm of paraffin-embedded human brain tissue (right) compared to a negative control without primary antibody (left). To expose target proteins, antigen retrieval was performed using 10 mM sodium citrate (pH 6.0), microwaved for 8-15 min. Following antigen retrieval, tissues were blocked in 3% H2O2-methanol for 15 min at room temperature, washed with ddH2O and PBS, and then probed with ab3465 diluted in 3% BSA-PBS at a dilution of 1/100 overnight at 4°C in a humidified chamber. Tissues were washed extensively in PBST and detection was performed using an HRP-conjugated secondary antibody followed by colorimetric detection using a DAB kit. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting.

  • Immunofluorescent analysis of CPEB (green) showing staining in the cytoplasm of U251 cells (right) compared to a negative control without primary antibody (left). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with ab3465 in 3% BSA-PBS at a dilution of 1/100 and incubated overnight at 4°C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight®-conjugated secondary antibody in PBS at room temperature in the dark. Actin was stained using Alexa Fluor® 554 (red) and nuclei were stained with Hoechst or DAPI (blue). Images were taken at a magnification of 60x.

  • Immunofluorescent analysis of CPEB (green) showing staining in the cytoplasm of C6 cells (right) compared to a negative control without primary antibody (left). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with ab3465 in 3% BSA-PBS at a dilution of 1/100 and incubated overnight at 4°C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight®-conjugated secondary antibody in PBS at room temperature in the dark. Nuclei were stained with Hoechst or DAPI (blue). Images were taken at a magnification of 60x.

  • Immunofluorescent analysis of CPEB (green) showing staining in the cytoplasm of HeLa cells (right) compared to a negative control without primary antibody (left). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with ab3465 in 3% BSA-PBS at a dilution of 1/100 and incubated overnight at 4°C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight®-conjugated secondary antibody in PBS at room temperature in the dark. Actin was stained using Alexa Fluor® 554 (red) and nuclei were stained with Hoechst or DAPI (blue). Images were taken at a magnification of 60x.

References for Anti-CPEB1 antibody (ab3465)

This product has been referenced in:
  • Chen J  et al. Genome-wide analysis of translation reveals a critical role for deleted in azoospermia-like (Dazl) at the oocyte-to-zygote transition. Genes Dev 25:755-66 (2011). WB ; Mouse . Read more (PubMed: 21460039) »

See 1 Publication for this product

Product Wall

Thank you for your enquiry and I'm sorry to hear that you are experiencing dificulty with this antibody. This is the first complaint that we have received regarding ab3465. At this point I would suggest increasing the concentration of ab3465 that you a...

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Thank you for your email. Unfortunately, we are not aware of any publications that feature the use of this antibody. It seems that ab3465 may cross-react with Xenopus CPEB, but I really can't say for sure. We do not routinely offer free or trial si...

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Thanks for your email. I'll try and answer your questionsa as fully as I can. 1) I have made enquiries about whether we can sell the MBD3 peptide. I hope this should be possible! I will let you know. 2) We would be interested in making phospho-sp...

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