Overview

  • Product nameAnti-CPEB1 antibody
    See all CPEB1 primary antibodies
  • Description
    Rabbit polyclonal to CPEB1
  • Tested applicationsSuitable for: WB, ICC/IF, ELISA, RIPmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    A 14 amino acid peptide from near the amino terminus of human CPEB1.

  • Positive control
    • Rat brain tissue lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab73287 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 63 kDa (predicted molecular weight: 63 kDa).
ICC/IF Use a concentration of 5 µg/ml.
ELISA Use at an assay dependent dilution.
RIP Use at an assay dependent dilution. PubMed: 20926679

Target

  • FunctionSequence-specific RNA-binding protein that regulates mRNA cytoplasmic polyadenylation and translation initiation during oocyte maturation, early development and at postsynapse sites of neurons. Binds to the cytoplasmic polyadenylation element (CPE), an uridine-rich sequence element (consensus sequence 5'-UUUUUAU-3') within the mRNA 3'-UTR. In absence of phosphorylation and in association with TACC3 is also involved as a repressor of translation of CPE-containing mRNA; a repression that is relieved by phosphorylation or degradation (By similarity). Involved in the transport of CPE-containing mRNA to dendrites; those mRNAs may be transported to dendrites in a translationally dormant form and translationally activated at synapses (By similarity). Its interaction with APLP1 promotes local CPE-containing mRNA polyadenylation and translation activation (By similarity). Induces the assembly of stress granules in the absence of stress.
  • Tissue specificityIsoform 1 is expressed in immature oocytes, ovary, brain and heart. Isoform 2 is expressed in brain and heart. Isoform 3 and isoform 4 are expressed in brain. Expressed in breast tumors and several tumor cell lines.
  • Sequence similaritiesBelongs to the RRM CPEB family.
    Contains 2 RRM (RNA recognition motif) domains.
  • DomainThe 2 RRM domains and the C-terminal region mediate interaction with CPE-containing RNA.
  • Post-translational
    modifications
    Phosphorylated on serine/threonine residues by AURKA/STK6 within positions 166 and 197. Phosphorylation and dephosphorylation on Thr-172 regulates cytoplasmic polyadenylation and translation of CPE-containing mRNAs. Phosphorylation on Thr-172 by AURKA/STK6 and CAMK2A activates CPEB1. Phosphorylation on Thr-172 may be promoted by APLP1. Phosphorylation increases binding to RNA.
  • Cellular localizationCytoplasm > P-body. Cytoplasmic granule. Cell junction > synapse. Membrane. Cell junction > synapse > postsynaptic cell membrane > postsynaptic density. Cell projection > dendrite. Also found in stress granules. Recruited to stress granules (SGs) upon arsenite treatment. In dendrites (By similarity). Localizes in synaptosomes at dendritic synapses of neurons (By similarity). Strongly enriched in postsynaptic density (PSD) fractions (By similarity). Transported into dendrites in a microtubule-dependent fashion and colocalizes in mRNA-containing particles with TACC3, dynein and kinesin (By similarity). Membrane-associated (By similarity). Colocalizes at excitatory synapses with members of the polyadenylation and translation complex factors (CPSF, APLP1, TACC3, AURKA/STK6, SYP, etc.) including CPE-containing RNAs.
  • Information by UniProt
  • Database links
  • Alternative names
    • CEBP antibody
    • CPE binding protein 1 antibody
    • CPE BP1 antibody
    • CPE-binding protein 1 antibody
    • CPE-BP1 antibody
    • CPEB 1 antibody
    • CPEB antibody
    • CPEB-1 antibody
    • CPEB1 antibody
    • CPEB1 protein antibody
    • CPEB1_HUMAN antibody
    • Cytoplasmic polyadenylation binding protein 1 antibody
    • Cytoplasmic polyadenylation element binding protein antibody
    • Cytoplasmic polyadenylation element binding protein 1 antibody
    • Cytoplasmic polyadenylation element-binding protein 1 antibody
    • FLJ13203 antibody
    • h CEBP antibody
    • h-CEBP antibody
    • hCPEB 1 antibody
    • hCPEB-1 antibody
    • MGC34136 antibody
    • MGC60106 antibody
    • mKIAA0940 antibody
    • MKIAA0940 protein antibody
    see all

Anti-CPEB1 antibody images

  • Lane 1 : Anti-CPEB1 antibody (ab73287) at 1 µg/ml
    Lane 2 : Anti-CPEB1 antibody (ab73287) at 2 µg/ml

    Lane 1 : rat brain tissue lysate
    Lane 2 : rat brain tissue lysate

    Lysates/proteins at 15 µg per lane.


    Predicted band size : 63 kDa
    Observed band size : 63 kDa
  • ICC/IF image of ab73287 stained PC12 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab73287, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

References for Anti-CPEB1 antibody (ab73287)

This product has been referenced in:
  • Alves-Sampaio A  et al. NMDA-mediated regulation of DSCAM dendritic local translation is lost in a mouse model of Down's syndrome. J Neurosci 30:13537-48 (2010). RIP ; Mouse . Read more (PubMed: 20926679) »

See 1 Publication for this product

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application Immunohistochemistry (Frozen sections)
Sample Mouse Tissue sections (Brain sections)
Specification Brain sections
Fixative Paraformaldehyde
Permeabilization Yes - Triton X100
Blocking step Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 22°C
Username

Mr. Amir Djourabchi

Verified customer

Submitted May 09 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"