Recombinant Anti-CPEB1 antibody [EPR11775(2)] (ab181051)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR11775(2)] to CPEB1
- Suitable for: ICC/IF, WB, IP
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-CPEB1 antibody [EPR11775(2)]
See all CPEB1 primary antibodies -
Description
Rabbit monoclonal [EPR11775(2)] to CPEB1 -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, WB, IPmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
(Peptide available asab197860) -
Positive control
- Human fetal brain, fetal heart, HeLa and A549 cell lysates. ICC/IF: HeLa cells
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 0.05% BSA, 59% PBS -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR11775(2) -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Immunizing Peptide (Blocking)
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Isotype control
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Positive Controls
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab181051 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ICC/IF |
1/100.
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WB |
1/1000 - 1/2000. Detects a band of approximately 70 kDa (predicted molecular weight: 63 kDa).Can be blocked with CPEB1 peptide (ab197860).
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IP |
1/60.
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Notes |
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ICC/IF
1/100. |
WB
1/1000 - 1/2000. Detects a band of approximately 70 kDa (predicted molecular weight: 63 kDa).Can be blocked with CPEB1 peptide (ab197860). |
IP
1/60. |
Target
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Function
Sequence-specific RNA-binding protein that regulates mRNA cytoplasmic polyadenylation and translation initiation during oocyte maturation, early development and at postsynapse sites of neurons. Binds to the cytoplasmic polyadenylation element (CPE), an uridine-rich sequence element (consensus sequence 5'-UUUUUAU-3') within the mRNA 3'-UTR. In absence of phosphorylation and in association with TACC3 is also involved as a repressor of translation of CPE-containing mRNA; a repression that is relieved by phosphorylation or degradation (By similarity). Involved in the transport of CPE-containing mRNA to dendrites; those mRNAs may be transported to dendrites in a translationally dormant form and translationally activated at synapses (By similarity). Its interaction with APLP1 promotes local CPE-containing mRNA polyadenylation and translation activation (By similarity). Induces the assembly of stress granules in the absence of stress. -
Tissue specificity
Isoform 1 is expressed in immature oocytes, ovary, brain and heart. Isoform 2 is expressed in brain and heart. Isoform 3 and isoform 4 are expressed in brain. Expressed in breast tumors and several tumor cell lines. -
Sequence similarities
Belongs to the RRM CPEB family.
Contains 2 RRM (RNA recognition motif) domains. -
Domain
The 2 RRM domains and the C-terminal region mediate interaction with CPE-containing RNA. -
Post-translational
modificationsPhosphorylated on serine/threonine residues by AURKA/STK6 within positions 166 and 197. Phosphorylation and dephosphorylation on Thr-172 regulates cytoplasmic polyadenylation and translation of CPE-containing mRNAs. Phosphorylation on Thr-172 by AURKA/STK6 and CAMK2A activates CPEB1. Phosphorylation on Thr-172 may be promoted by APLP1. Phosphorylation increases binding to RNA. -
Cellular localization
Cytoplasm > P-body. Cytoplasmic granule. Cell junction > synapse. Membrane. Cell junction > synapse > postsynaptic cell membrane > postsynaptic density. Cell projection > dendrite. Also found in stress granules. Recruited to stress granules (SGs) upon arsenite treatment. In dendrites (By similarity). Localizes in synaptosomes at dendritic synapses of neurons (By similarity). Strongly enriched in postsynaptic density (PSD) fractions (By similarity). Transported into dendrites in a microtubule-dependent fashion and colocalizes in mRNA-containing particles with TACC3, dynein and kinesin (By similarity). Membrane-associated (By similarity). Colocalizes at excitatory synapses with members of the polyadenylation and translation complex factors (CPSF, APLP1, TACC3, AURKA/STK6, SYP, etc.) including CPE-containing RNAs. - Information by UniProt
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Database links
- Entrez Gene: 64506 Human
- Omim: 607342 Human
- SwissProt: Q9BZB8 Human
- Unigene: 547988 Human
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Alternative names
- CEBP antibody
- CPE binding protein 1 antibody
- CPE BP1 antibody
see all
Images
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Immunocytochemistry analysis of 100% methanol-fixed 0.1% TritonX-100 permeabilized HeLa cells staining CPEB1 with ab181051 at 1/100 dilution (5.44 μg/mL) and secondary: ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 (2 μg/mL), and counterstained with ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) at 1/200 (2.5 μg/mL) dilution. Nuclear counterstain is DAPI (blue).
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All lanes : Anti-CPEB1 antibody [EPR11775(2)] (ab181051) at 1/1000 dilution
Lane 1 : Human fetal brain tissue lysate
Lane 2 : Human fetal heart tissue lysate
Lane 3 : HeLa cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab136636) at 1/500 dilution
Predicted band size: 63 kDa -
Anti-CPEB1 antibody [EPR11775(2)] (ab181051) at 1/1000 dilution + A549 cell lysate at 10 µg
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 63 kDa -
Western blot analysis of immunoprecipitation pellet from Human fetal brain lysate immunoprecipitated using ab181051 at 1/60 dilution.
Secondary: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (2)
ab181051 has been referenced in 2 publications.
- Jia Y et al. miR-301 regulates the SIRT1/SOX2 pathway via CPEB1 in the breast cancer progression. Mol Ther Oncolytics 22:13-26 (2021). PubMed: 34377766
- Luong XG et al. Genome-wide analysis reveals a switch in the translational program upon oocyte meiotic resumption. Nucleic Acids Res 48:3257-3276 (2020). PubMed: 31970406