Cytoplasmic polyadenylation element binding protein 3 antibody
Cytoplasmic polyadenylation element-binding protein 3 antibody
Immunohistochemistry (PFA perfusion fixed frozen sections) - CPEB3 antibody (ab10883)This image is courtesy of an Abreview submitted by Dr Sophie Pezet
ab10883 staining CPEB3 in mouse brain tissue section by Immunohistochemistry (PFA perfusion fixed frozen sections). Tissue from 4% PFA perfused animals underwent overnight fixation in 4% paraformaldehyde, cryoprotected in 30% sucrose and cut using cryostat.The primary antibody was diluted, 1/100 (PBS + 0.3% Triton X100) and incubated with sample for 18 hours at 20°C. An Alexa Fluor®488 conjugated goat polyclonal to rabbit IgG at 1/1000 dilution, was used as secondary.
ICC/IF image of ab10883 stained SKNSH cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab10883 at 5µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Western blot - CPEB3 antibody (ab10883)
Lane 1 : Anti-CPEB3 antibody (ab10883) at 1/500 dilution Lane 2 : Anti-CPEB3 antibody (ab10883) at 1/1000 dilution
Lane 1 : Mouse Brain lysate Lane 2 : Mouse Brain lysate
We think that the top band here is the correct band, as it is the same size as the most intense band on the previous blot. The banding pattern is the same on both blots and the lower bands may be clevage products as there is no evidence of CPEB3 isoforms in the literature.