Anti-CPEB4 antibody (ab83009)

Overview

  • Product nameAnti-CPEB4 antibody
    See all CPEB4 primary antibodies
  • Description
    Rabbit polyclonal to CPEB4
  • Tested applicationsSuitable for: IHC-Fr, WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Guinea pig, Cow, Cat, Dog
  • Immunogen

    Synthetic peptide corresponding to N terminal amino acids 72-121 (DEILGSEKAK SQQQEQQDPL EKQQLSPSPG QEAGILPETE KAKSEENQGD) of human CPEB4 (NP_085130).

  • Positive control
    • Fetal lung lysate

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferPreservative: None
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab83009 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr Use at an assay dependent concentration.
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 80 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

Target

Anti-CPEB4 antibody images

  • Anti-CPEB4 antibody (ab83009) at 1 µg/ml + fetal lung lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 80 kDa
    Observed band size : 85 kDa (why is the actual band size different from the predicted?)

References for Anti-CPEB4 antibody (ab83009)

ab83009 has not yet been referenced specifically in any publications.

Product Wall

Thank you for your email. I am sorry for all the inconvenience. I am still expecting an answer from the laboratory. As requested, I have issued a free of charge replacement with one vial of ab83009 lot x with the order number x. The vial should arrive ...

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Thank you for calling us and for alerting usof the problem you are experiencing withthe new batch of this antibody. We take product complaints very seriously, and investigate every product and batch that we feel may not be performing correctly.
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Muchas gracias por haberte tomado la molestia de enviarnos el cuestionario y las imágenes adjuntas.

Efectivamente, comparando las imágenes de los dos lotes, el último no está dando la señal esperada. He veri...

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Gracias por tu respuesta y por confirmar los detalles del pedido.

Ya he realizado el nuevo pedido completamente gratuito del anticuerpo ab83009.

Recibiréis una confirmación automática del mismo con más de...

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Muchas gracias por contactarnos y alertarnos del exceso de volumen recibido.

He contactado con mis compañeros encargados de alicuotar los anticuerpos, y me han comentado que normalmente suele haber un exceso de volumen de anticuerpo pa...

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Application Immunohistochemistry (Frozen sections)
Sample Mouse Tissue sections (Brain sections)
Specification Brain sections
Fixative Paraformaldehyde
Permeabilization Yes - Triton X100
Blocking step Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 22°C
Username

Mr. Amir Djourabchi

Verified customer

Submitted Apr 13 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"