Overview

  • Product name
    Anti-CPEB4 antibody - N-terminal
    See all CPEB4 primary antibodies
  • Description
    Rabbit polyclonal to CPEB4 - N-terminal
  • Host species
    Rabbit
  • Tested applications
    Suitable for: ICC/IF, WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Recombinant fragment, corresponding to a region within N terminal amino acids 1-172 of Human CPEB4 (UniProt ID: Q17RY0).

  • Positive control
    • NT2D1, PC3, U87 MG, SK-N-SH whole cell lysate; U87 xenograft This antibody gave a positive result when used in the following methanol fixed cell lines: HeLa
  • General notes
    Keep as a concentrated solution.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    pH: 7.00
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 78% PBS, 20% Glycerol, 1% BSA
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab155204 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 5 µg/ml.
WB 1/5000 - 1/20000. Predicted molecular weight: 80 kDa.
IHC-P 1/100 - 1/1000.

Target

Images

  • ICC/IF image of ab155204 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab155204 at 5µg/ml overnight at +4°C. The secondary antibody (pseudo-colored green) was Dylight® 488 goat anti- rabbit (ab96899) IgG (H+L) preadsorbed, used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (pseudo-colored red) at a 1/200 dilution for 1h at room temperature. DAPI was used to stain the cell nuclei (pseudo-colored blue) at a concentration of 1.43µM for 1hour at room temperature.

  • All lanes : Anti-CPEB4 antibody - N-terminal (ab155204) at 1/10000 dilution

    Lane 1 : NT2D1 whole cell lysate
    Lane 2 : PC3 whole cell lysate
    Lane 3 : U87-MG whole cell lysate
    Lane 4 : Sk-N-SH whole cell lysate

    Lysates/proteins at 30 µg per lane.

    Predicted band size: 80 kDa



    7.5% SDS PAGE
  • Immunohistochemical analysis of paraffin embedded U87 xenograft tissue labeling CPEB4 with ab155204 at 1/500 dilution.

References

ab155204 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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