Overview

  • Product nameAnti-CPI17 alpha antibody [E305]
    See all CPI17 alpha primary antibodies
  • Description
    Rabbit monoclonal [E305] to CPI17 alpha
  • Specificityab32213 recognises CPI17 alpha and may detect isoform CPI17 beta.
  • Tested applicationsSuitable for: WB, IPmore details
    Unsuitable for: ICC or IHC-P
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to Human CPI17 alpha aa 1-100 (N terminal).

  • Epitopeab32213 reacts with an epitope located in the N terminal region of CPI17 alpha
  • Positive control
    • Hela, Jurkat, MCF-7 and A431 cell lysate
  • General notes

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated ‘PUR’ on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage bufferpH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
  • Concentration information loading...
  • PurityProtein A purified
  • ClonalityMonoclonal
  • Clone numberE305
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab32213 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/5000. Detects a band of approximately 17 kDa (predicted molecular weight: 17 kDa).
IP 1/80.
  • Application notesIs unsuitable for ICC or IHC-P.
  • Target

    • FunctionInhibitor of PPP1CA. Has over 1000-fold higher inhibitory activity when phosphorylated, creating a molecular switch for regulating the phosphorylation status of PPP1CA substrates and smooth muscle contraction.
    • Tissue specificityIsoform 1 is detected in aorta and testis. Isoform 2 is detected in aorta.
    • Sequence similaritiesBelongs to the PP1 inhibitor family.
    • Cellular localizationCytoplasm.
    • Information by UniProt
    • Database links
    • Alternative names
      • 17 kDa PKC-potentiated inhibitory protein of PP1 antibody
      • 17-KDa protein antibody
      • 17kDa PKC potentiated inhibitory protein of PP1 antibody
      • CPI 17 alpha antibody
      • CPI 17 antibody
      • CPI 17alpha antibody
      • CPI-17 antibody
      • CPI17 antibody
      • PKC potentiated inhibitory protein of PP1 antibody
      • PKC potentiated inhibitory protein of PP1, 17-KD antibody
      • PP14A_HUMAN antibody
      • PPP1INL antibody
      • Ppp1r14a antibody
      • Protein kinase C-potentiated inhibitor of protein phosphatase 1, 17-KD antibody
      • Protein kinase C-potentiated inhibitor protein of 17 kDa antibody
      • Protein phosphatase 1 antibody
      • Protein phosphatase 1 regulatory (inhibitor) subunit 14A antibody
      • Protein phosphatase 1 regulatory subunit 14A antibody
      • Regulatory subunit 14A antibody
      see all

    Anti-CPI17 alpha antibody [E305] images

    • Lanes 1, 2 & 4 : purified at 1/1000 dilution
      Lane 3 : RAW264.7 purified at 1/1000 dilution

      Lane 1 : C6 cell lysate
      Lane 2 : PC-12 cell lysate
      Lane 3 : cell lysate
      Lane 4 : NIH/3T3 cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      HRP goat anti-rabbit (H+L) at 1/1000 dilution

      Predicted band size : 17 kDa
      Observed band size : 17 kDa

      Blocking buffer: 5% NFDM/TBST

      Dilution buffer: 5% NFDM/TBST

    • Anti-CPI17 alpha antibody [E305] (ab32213) at 1/1000 dilution (purified) + Jurkat cell lysate at 10 µg

      Secondary
      HRP goat anti-rabbit (H+L) at 1/1000 dilution

      Predicted band size : 17 kDa
      Observed band size : 17 kDa
    • ab32213 (purified) at 1/50 immunoprecipitating CPI17 in MCF7 cell lysate (Lane 1). Lane 2 - PBS. For western blotting, a HRP-conjugated anti-rabbit IgG, specific to the non-reduced form of IgG was used as the secondary antibody (1/1500).

      Blocking buffer and concentration: 5% NFDM/TBST.

      Diluting buffer and concentration: 5% NFDM /TBST.

    • Anti-CPI17 alpha antibody [E305] (ab32213) at 1/1000 dilution (unpurified) + Jurkat cell lysate at 10 µg

      Secondary
      HRP goat anti-rabbit (H+L) at 1/1000 dilution

      Predicted band size : 17 kDa
      Observed band size : 17 kDa

      Blocking buffer: 5% NFDM/TBST

      Dilution buffer: 5% NFDM/TBST

    • All lanes : Anti-CPI17 alpha antibody [E305] (ab32213) at 1/5000 dilution (unpurified)

      Lane 1 : HeLa cell lysate
      Lane 2 : Jurkat cell lysate
      Lane 3 : MCF-7 cell lysate
      Lane 4 : A431 cell lysate


      Predicted band size : 17 kDa
      Observed band size : 17 kDa
    • ab32213 (unpurified) at 1/50 immunoprecipitating CPI17 in MCF7 cell lysate (Lane 1). Lane 2 - PBS. For western blotting, a HRP-conjugated anti-rabbit IgG, specific to the non-reduced form of IgG was used as the secondary antibody (1/1500).

      Blocking buffer and concentration: 5% NFDM/TBST.

      Diluting buffer and concentration: 5% NFDM /TBST.

    References for Anti-CPI17 alpha antibody [E305] (ab32213)

    This product has been referenced in:
    • Kang S  et al. Dysfunction of vascular smooth muscle and vascular remodeling by simvastatin. Toxicol Sci 138:446-556 (2014). Rat . Read more (PubMed: 24449418) »
    • Lima VV  et al. Vascular O-GlcNAcylation augments reactivity to constrictor stimuli by prolonging phosphorylated levels of the myosin light chain. Braz J Med Biol Res 47:826-33 (2014). WB ; Rat . Read more (PubMed: 25140811) »

    See all 2 Publications for this product

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    There are currently no Abreviews or Questions for ab32213.
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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"