Anti-CPI17 alpha (phospho T38) antibody (ab52174)

Overview

  • Product name
    Anti-CPI17 alpha (phospho T38) antibody
    See all CPI17 alpha primary antibodies
  • Description
    Rabbit polyclonal to CPI17 alpha (phospho T38)
  • Specificity
    ab52174 detects endogenous levels of CPI17a only when phosphorylated at threonine 38.
  • Tested applications
    Suitable for: IHC-P, ELISA, WBmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic phosphopeptide derived from human CPI17a around the phosphorylation site of threonine 38 (R-V-TP-V-K).

  • Positive control
    • Human breast carcinoma tissue.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Storage buffer
    Preservative: 0.02% Sodium Azide
    Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    ab52174 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab52174 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent concentration.
ELISA 1/20000.
WB 1/1000. Detects a band of approximately 17 kDa (predicted molecular weight: 17 kDa).

Target

  • Function
    Inhibitor of PPP1CA. Has over 1000-fold higher inhibitory activity when phosphorylated, creating a molecular switch for regulating the phosphorylation status of PPP1CA substrates and smooth muscle contraction.
  • Tissue specificity
    Isoform 1 is detected in aorta and testis. Isoform 2 is detected in aorta.
  • Sequence similarities
    Belongs to the PP1 inhibitor family.
  • Cellular localization
    Cytoplasm.
  • Information by UniProt
  • Database links
  • Alternative names
    • 17 kDa PKC-potentiated inhibitory protein of PP1 antibody
    • 17-KDa protein antibody
    • 17kDa PKC potentiated inhibitory protein of PP1 antibody
    • CPI 17 alpha antibody
    • CPI 17 antibody
    • CPI 17alpha antibody
    • CPI-17 antibody
    • CPI17 antibody
    • PKC potentiated inhibitory protein of PP1 antibody
    • PKC potentiated inhibitory protein of PP1, 17-KD antibody
    • PP14A_HUMAN antibody
    • PPP1INL antibody
    • Ppp1r14a antibody
    • Protein kinase C-potentiated inhibitor of protein phosphatase 1, 17-KD antibody
    • Protein kinase C-potentiated inhibitor protein of 17 kDa antibody
    • Protein phosphatase 1 antibody
    • Protein phosphatase 1 regulatory (inhibitor) subunit 14A antibody
    • Protein phosphatase 1 regulatory subunit 14A antibody
    • Regulatory subunit 14A antibody
    see all

Anti-CPI17 alpha (phospho T38) antibody images

  • ab52174 at 1/50 dilution staining CPI17a in human breast carcinoma by Immunohistochemistry, paraffin embedded tissue, in the presence (right image) and absence (left image) of the immunising peptide.


  • Predicted band size : 17 kDa

References for Anti-CPI17 alpha (phospho T38) antibody (ab52174)

This product has been referenced in:
  • Kang S  et al. Dysfunction of vascular smooth muscle and vascular remodeling by simvastatin. Toxicol Sci 138:446-556 (2014). Rat . Read more (PubMed: 24449418) »

See 1 Publication for this product

Product Wall

Thank you for contacting us.

We have not specifically tested ab52147 on C2C12 cells. However, we do have customer feedback regarding the use of this antibody to detect the phosphorylated protein in treated myometrial smooth muscle cells. Read More

Thank you for contacting us.
This antibody has been validated for Western blot using RAW264.7 cell lysate. It has not been tested against other mouse cell lines, such as C2C12.


I hope this information is helpful to you. Please do ...

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (Myometrial smooth muscle cells)
Loading amount
15 µg
Specification
Myometrial smooth muscle cells
Treatment
1 or 10 nM oxytocin for 1 minute, and 1µM phorbol 12-myristate 13-acetate for 10 minutes
Gel Running Conditions
Reduced Denaturing (13% tris-glycine SDS-PAGE gel)
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Username

Abcam user community

Verified customer

Submitted Apr 10 2009

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up