Overview

  • Product name
  • Description
    Rabbit polyclonal to cPLA2 beta
  • Tested applications
    Suitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant full length protein without the signal sequence and activation peptide, (Human) with an additional 16 residue histidine tag

Properties

Applications

Our Abpromise guarantee covers the use of ab53421 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 114 kDa.
ELISA Use at an assay dependent dilution.

Target

  • Relevance
    This gene encodes a member of the cytosolic phospholipase A2 protein family. Phospholipase A2 enzymes hydrolyze the sn-2 bond of phospholipids, releasing lysophospholipids and fatty acids. This enzyme may be associated with mitochondria and early endosomes. Most tissues also express read-through transcripts from the upstream gene into this gene, some of which may encode fusion proteins combining the N-terminus of the upstream gene including its JmjC domain with the almost complete coding region of this gene, including the C2 and cytoplasmic phospholipase A2 domains.
  • Cellular localization
    Cytoplasmic and Mitochondrial
  • Database links
  • Alternative names
    • CPLA2 beta antibody
    • Cytosolic phospholipase A2 beta antibody
    • DKFZp686N12131 antibody
    • FLJ17693 antibody
    • FLJ42498 antibody
    • FLJ53173 antibody
    • HsT16992 antibody
    • OTTHUMP00000201605 antibody
    • OTTHUMP00000210673 antibody
    • Phospholipase A2 group IVB antibody
    • Phospholipase A2, group IVB (cytosolic) antibody
    • PLA2G4B antibody
    see all

Anti-cPLA2 beta antibody images

  • Anti-cPLA2 beta antibody (ab53421) at 1/1000 dilution (in 1% Marvel + TBST for 1 hour at 22°C ) + Whole cell lysate of Human A549 cells at 10 µg

    Secondary
    An HRP-conjugated donkey anti rabbit polyclonal at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 114 kDa
    Observed band size : 60 kDa (why is the actual band size different from the predicted?)


    Exposure time : 8 minutes

    This image is courtesy of an anonymous Abreview

References for Anti-cPLA2 beta antibody (ab53421)

ab53421 has not yet been referenced specifically in any publications.

Product Wall

Application
Western blot
Sample
Human Tissue lysate - whole (Muscle)
Gel Running Conditions
Reduced Denaturing (12%)
Loading amount
20 µg
Specification
Muscle
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Jul 10 2015

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (A549 cells)
Loading amount
10 µg
Specification
A549 cells
Gel Running Conditions
Reduced Denaturing (10%)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Username

Abcam user community

Verified customer

Submitted Oct 18 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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