Anti-Cpn10 antibody (ab90494)

Overview

  • Product name
  • Description
    Rabbit polyclonal to Cpn10
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
    Predicted to work with: Sheep, Rabbit, Guinea pig, Cow, Dog, Pig, Xenopus laevis
  • Immunogen

    Synthetic peptide derived from sequence near the C terminal of Saccharomyces cerevisiae Cpn10, conjugated to KLH.

  • Positive control
    • HeLa cell lysate (heat shocked)

Properties

Applications

Our Abpromise guarantee covers the use of ab90494 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000. Predicted molecular weight: 11 kDa.

Target

  • Function
    Eukaryotic CPN10 homolog which is essential for mitochondrial protein biogenesis, together with CPN60. Binds to CPN60 in the presence of Mg-ATP and suppresses the ATPase activity of the latter.
  • Sequence similarities
    Belongs to the GroES chaperonin family.
  • Cellular localization
    Mitochondrion matrix.
  • Information by UniProt
  • Database links
  • Alternative names
    • 10 kDa chaperonin antibody
    • 10 kDa heat shock protein mitochondrial antibody
    • 10 kDa heat shock protein, mitochondrial antibody
    • CH10_HUMAN antibody
    • Chaperonin 10 antibody
    • Chaperonin 10 homolog antibody
    • CPN10 antibody
    • cpn10 homolog antibody
    • Early pregnancy factor antibody
    • Early-pregnancy factor antibody
    • EPF antibody
    • GROES antibody
    • GroES homolog antibody
    • Heat shock 10kD protein 1 chaperonin 10 antibody
    • Heat shock 10kDa protein 1 antibody
    • Heat shock 10kDa protein 1 chaperonin 10 antibody
    • Heat shock protein family E (Hsp10) member antibody
    • Heat-shock 10-kD protein antibody
    • Hsp10 antibody
    • Hspe1 antibody
    see all

Anti-Cpn10 antibody images

  • Anti-Cpn10 antibody (ab90494) at 1/2000 dilution + HeLa cell lysate (heat shocked)

    Predicted band size : 11 kDa

References for Anti-Cpn10 antibody (ab90494)

ab90494 has not yet been referenced specifically in any publications.

Product Wall

Thank you for your reply and I apologize for the delay as I was awaiting more information from the lab. They were unable to BLAST the sequence agains the rrequested species, but they did offer this information below:

Please see thelink below f...

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Thank you for contacting us.

We do not make this antibody in-house and unfortunately the supplying lab considers the exact immunogen sequence to be proprietary. The only information we know is thatthe immunogen that was used to raise the ant...

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Thank you for your inquiry.

This antibody is a polyclonal so it will have multiple binding epitopes. However, the immunogen that was used to raise the antibody corresponds to a sequence near the C terminal of Saccharomyces cerevisiae Cpn10. <...

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Abreviews
Abcam has not validated the combination of species/application used in this Abreview.
Application
Western blot
Sample
Pig Tissue lysate - whole (uterus)
Loading amount
50 µg
Specification
uterus
Gel Running Conditions
Reduced Denaturing
Blocking step
Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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Verified customer

Submitted Sep 02 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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