Recombinant
RabMAb

Anti-Cpn10 antibody [EPR4475] (ab108611)

Overview

  • Product name
    Anti-Cpn10 antibody [EPR4475]
    See all Cpn10 primary antibodies
  • Description
    Rabbit monoclonal [EPR4475] to Cpn10
  • Tested applications
    Suitable for: WB, IP, Flow Cytmore details
    Unsuitable for: ICC or IHC-P
  • Species reactivity
    Reacts with: Rat, Human
  • Immunogen

    A synthetic peptide corresponding to residues in Human Cpn10.

  • Positive control
    • HeLa, HepG2, Raji, and Jurkat cell lysates. Permeabilized Jurkat cells.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Mouse: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

Properties

Applications

Our Abpromise guarantee covers the use of ab108611 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 11 kDa (predicted molecular weight: 11 kDa).
IP 1/10 - 1/100.
Flow Cyt 1/10 - 1/100. ab172730-Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
  • Application notes
    Is unsuitable for ICC or IHC-P.
  • Target

    • Function
      Eukaryotic CPN10 homolog which is essential for mitochondrial protein biogenesis, together with CPN60. Binds to CPN60 in the presence of Mg-ATP and suppresses the ATPase activity of the latter.
    • Sequence similarities
      Belongs to the GroES chaperonin family.
    • Cellular localization
      Mitochondrion matrix.
    • Information by UniProt
    • Database links
    • Alternative names
      • 10 kDa chaperonin antibody
      • 10 kDa heat shock protein mitochondrial antibody
      • 10 kDa heat shock protein, mitochondrial antibody
      • CH10_HUMAN antibody
      • Chaperonin 10 antibody
      • Chaperonin 10 homolog antibody
      • CPN10 antibody
      • cpn10 homolog antibody
      • Early pregnancy factor antibody
      • Early-pregnancy factor antibody
      • EPF antibody
      • GROES antibody
      • GroES homolog antibody
      • Heat shock 10kD protein 1 chaperonin 10 antibody
      • Heat shock 10kDa protein 1 antibody
      • Heat shock 10kDa protein 1 chaperonin 10 antibody
      • Heat shock protein family E (Hsp10) member antibody
      • Heat-shock 10-kD protein antibody
      • Hsp10 antibody
      • Hspe1 antibody
      see all

    Images

    • All lanes : Anti-Cpn10 antibody [EPR4475] (ab108611) at 1/1000 dilution

      Lane 1 : HeLa cell lysate
      Lane 2 : HepG2 cell lysate
      Lane 3 : Raji cell lysate
      Lane 4 : Jurkat cell lysate

      Lysates/proteins at 10 µg per lane.


      Predicted band size : 11 kDa
      Observed band size : 11 kDa
    • Flow cytometric analysis of permeabilized Jurkat cells using ab108611 at 1/10 (red) or a Rabbit IgG (negative) (green).

    References

    ab108611 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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