Overview

  • Product nameAnti-Cpn10 antibody [EPR4476]
    See all Cpn10 primary antibodies
  • Description
    Rabbit monoclonal [EPR4476] to Cpn10
  • Tested applicationsSuitable for: WB, IP, IHC-P, ICCmore details
    Unsuitable for: Flow Cyt
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide corresponding to residues in Human Cpn10.

  • Positive control
    • WB: A431, Neuro-2a, A549, and MCF7 cell lysates IHC-P: Human colon and kidney tissues ICC/IF: HeLa cells
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

Properties

Applications

Our Abpromise guarantee covers the use of ab108600 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/10000 - 1/50000. Detects a band of approximately 11 kDa (predicted molecular weight: 11 kDa).
IP 1/10 - 1/100.
IHC-P 1/250 - 1/500.
ICC 1/100 - 1/250.
  • Application notesIs unsuitable for Flow Cyt.
  • Target

    • FunctionEukaryotic CPN10 homolog which is essential for mitochondrial protein biogenesis, together with CPN60. Binds to CPN60 in the presence of Mg-ATP and suppresses the ATPase activity of the latter.
    • Sequence similaritiesBelongs to the GroES chaperonin family.
    • Cellular localizationMitochondrion matrix.
    • Information by UniProt
    • Database links
    • Alternative names
      • 10 kDa chaperonin antibody
      • 10 kDa heat shock protein mitochondrial antibody
      • 10 kDa heat shock protein, mitochondrial antibody
      • CH10_HUMAN antibody
      • Chaperonin 10 antibody
      • Chaperonin 10 homolog antibody
      • CPN10 antibody
      • cpn10 homolog antibody
      • Early pregnancy factor antibody
      • Early-pregnancy factor antibody
      • EPF antibody
      • GROES antibody
      • GroES homolog antibody
      • Heat shock 10kD protein 1 chaperonin 10 antibody
      • Heat shock 10kDa protein 1 antibody
      • Heat shock 10kDa protein 1 chaperonin 10 antibody
      • Heat-shock 10-kD protein antibody
      • Hsp10 antibody
      • Hspe1 antibody
      see all

    Anti-Cpn10 antibody [EPR4476] images

    • All lanes : Anti-Cpn10 antibody [EPR4476] (ab108600) at 1/10000 dilution

      Lane 1 : A431 cell lysate
      Lane 2 : Neuro-2a cell lysate
      Lane 3 : A549 cell lysate
      Lane 4 : MCF7 cell lysate

      Lysates/proteins at 10 µg per lane.


      Predicted band size : 11 kDa
      Observed band size : 11 kDa
    • Immunohistochemical staining of Cpn10 in paraffin embedded Human colon tissue, using ab108600 at a dilution of 1/250.
    • Immunohistochemical staining of Cpn10 in paraffin embedded Human kidney tissue, using ab108600 at a dilution of 1/250.
    • Immunofluorescent staining of Cpn10 in HeLa cells, using ab108600 at a dilution of 1/100.
    • ab108600 showing positive staining in Normal uterus tissue.

    • ab108600 showing positive staining in Ovarian carcinoma tissue.

    • ab108600 showing positive staining in Normal breast tissue.

    • ab108600 showing positive staining in Colonic adenocarcinoma tissue.

    • ab108600 showing positive staining in Normal stomach tissue.

    References for Anti-Cpn10 antibody [EPR4476] (ab108600)

    This product has been referenced in:
    • Sakellariou GK  et al. Neuron-specific expression of CuZnSOD prevents the loss of muscle mass and function that occurs in homozygous CuZnSOD-knockout mice. FASEB J 28:1666-81 (2014). WB ; Mouse . Read more (PubMed: 24378874) »
    • Gispert S  et al. Loss of mitochondrial peptidase Clpp leads to infertility, hearing loss plus growth retardation via accumulation of CLPX, mtDNA and inflammatory factors. Hum Mol Genet N/A:N/A (2013). Read more (PubMed: 23851121) »

    See all 2 Publications for this product

    Product Wall

    Application Western blot
    Sample Rat Tissue lysate - whole (Skeletal muscle)
    Gel Running Conditions Reduced Denaturing (15%)
    Loading amount 20 µg
    Specification Skeletal muscle
    Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
    Username

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    Verified customer

    Submitted Feb 04 2016

    Application Immunoprecipitation
    Immuno-precipitation step Protein A
    Sample Human Cell lysate - whole cell (HEK293 cells)
    Specification HEK293 cells
    Total protein in input 250 µg
    Username

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    Verified customer

    Submitted Sep 29 2014

    Application Western blot
    Loading amount 50 µg
    Gel Running Conditions Reduced Denaturing (15% gel)
    Sample Mouse Tissue lysate - whole (Skeletal muscle)
    Specification Skeletal muscle
    Blocking step Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
    Username

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    Verified customer

    Submitted Jan 09 2014

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Western blot
    Sample Human Cell lysate - whole cell (Jurkat)
    Loading amount 10 µg
    Specification Jurkat
    Gel Running Conditions Reduced Denaturing
    Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 4% · Temperature: 25°C
    Username

    Abcam user community

    Verified customer

    Submitted May 02 2012

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"