Overview

  • Product nameAnti-CPSF1 antibody
    See all CPSF1 primary antibodies
  • Description
    Rabbit polyclonal to CPSF1
  • Tested applicationsSuitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    A region within synthetic peptide: GCYDMWTVIA PVRKEEEDNP KGEGTEQEPS TTPEADDDGR RHGFLILSRE, corresponding to amino acids 531-580 of Human CPSF1

  • Positive control
    • 293T cell lysate.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage bufferPreservative: None
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab62598 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.5 µg/ml. Detects a band of approximately 144 kDa (predicted molecular weight: 161 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA Use at an assay dependent concentration.

ELISA titre using peptide based assay: 1/62500.

Target

  • RelevanceCPSF1 (Cleavage and polyadenylation specificity factor subunit 1) is a component of the cleavage and polyadenylation specificity factor (CPSF) complex. It plays a key role in pre-mRNA 3'-end formation, recognizing the AAUAAA signal sequence and interacting with poly(A) polymerase and other factors to bring about cleavage and poly(A) addition. This subunit is involved in the RNA recognition step of the polyadenylation reaction.
  • Cellular localizationNucleus, nucleoplasm.
  • Database links
  • Alternative names
    • Cleavage and polyadenylation specificity factor 160 kDa subunit antibody
    • Cleavage and polyadenylation specificity factor subunit 1 antibody
    • CPSF 160 kDa subunit antibody
    • CPSF160 antibody
    • HSU37012 antibody
    • P/cl.18 antibody
    see all

Anti-CPSF1 antibody images

  • Anti-CPSF1 antibody (ab62598) at 0.5 µg/ml + 293T cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 161 kDa
    Observed band size : 144 kDa (why is the actual band size different from the predicted?)

References for Anti-CPSF1 antibody (ab62598)

ab62598 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"