The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application notesICC/IF: 1/100.
IHC-P: 1/100 - 1/500. Epitope exposure is recommended. Epitope exposure with citrate will enhance staining.
Likely to work with frozen sections.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
RelevanceCPSF1 (Cleavage and polyadenylation specificity factor subunit 1) is a component of the cleavage and polyadenylation specificity factor (CPSF) complex. It plays a key role in pre-mRNA 3'-end formation, recognizing the AAUAAA signal sequence and interacting with poly(A) polymerase and other factors to bring about cleavage and poly(A) addition. This subunit is involved in the RNA recognition step of the polyadenylation reaction.
Detection of Human CPSF1 by IHC-IF. Sample: FFPE section of Human breast carcinoma.Antibody: ab84743 used at a dilution of 1/100. Detection: Red-fluorescent goat anti-rabbit IgG highly cross-adsorbed Antibody Hilyte PlusTM 555 used at a dilution of 1/100.